- 著者
- Takashi Onozaki Masahiko Yamada Masafumi Yagi Koji Tanase Michio Shibata
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-080, (Released:2017-04-05)
- 被引用文献数
- 7
Flower vase life of cut ornamental flowers, including carnations, is important in determining their quality and consumer preference for cut flowers. Therefore, we repeatedly crossed and selected promising offspring with long vase life for seven generations, from 1992 to 2008, in order to improve the vase life of carnation (Dianthus caryophyllus L.) flowers using conventional cross-breeding techniques. We investigated flower vase life, ethylene production at natural senescence, ethylene production after ethylene treatment (autocatalytic ethylene biosynthesis), response time to ethylene treatment (ethylene sensitivity), and flower diameter in six carnation cultivars used for initial breeding materials and a total of 123 selected lines from the first to seventh generations. Our results indicate that there was a large genetic variability in the five traits. Significant negative correlations were observed between flower vase life and ethylene production at natural senescence (r = −0.88**), between flower vase life and ethylene production after ethylene treatment (r = −0.90**), and between flower vase life and flower diameter (r = −0.92**). However, there was no correlation between flower vase life and ethylene sensitivity. Thus, the long vase life of selected carnation lines was strongly associated with a decrease in their ethylene production. Our results indicate that variation in flower vase life is not due to differences in ethylene sensitivity, but due to differences in ethylene production. Although flower size is an important floricultural trait for commercial production, crossing and selection for flower vase life resulted in a considerable reduction in flower diameter. The course of mean flower vase life over generations showed that selection was effectively made in the first to sixth generations. Repeatability for the flower vase life was roughly constant (0.3 to 0.4) in the first to sixth generations, but decreased to 0.12 in the seventh generation, suggesting a decrease in genetic variation in the seventh generation.
- 著者
- Koji Tanase Takashi Onozaki
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.MI-093, (Released:2015-12-11)
- 被引用文献数
- 6
We investigated differences in flower longevity, ethylene production, and ethylene sensitivity among pot carnation cultivars by quantitative PCR analysis. The flower life of ‘Polaris’ was significantly longer than that of ‘Ariel’ (control cultivar), ‘Orange Duo’, and ‘Lemon Soft’. The flowers of ‘Polaris’ produced little ethylene, and had low expression of the ethylene biosynthesis genes DcACS1 and DcACO1. The transcript levels of senescence-related (SR) genes DcCP1, DcbGal, and DcGST1 increased in petals of ‘Ariel’, ‘Orange Duo’, and ‘Lemon Soft’ at senescence, but were very low in ‘Polaris’. These results suggest that the low ethylene production in ‘Polaris’ is caused by low expression of DcACS1 and DcACO1, as in long-life flowers of the cut carnation cultivars ‘Miracle Rouge’ and ‘Miracle Symphony’. The ethylene sensitivity of ‘Orange Duo’ and ‘Lemon Soft’ was lower than that of both ‘Ariel’ and ‘Polaris’. Petals of ‘Orange Duo’ and ‘Lemon Soft’ wilted, and inrolled more slowly than those of ‘Ariel’ and ‘Polaris’, despite the upregulation of ethylene biosynthesis genes (DcACS1 and DcACO1), DcCP1, DcbGal, DcGST1, and DcEIL3 in petals of all cultivars upon application of exogenous ethylene. These results imply that only the components related to the inrolling of petals are altered in these ethylene-insensitive cultivars.