- 著者
- Takashi Onozaki Masahiko Yamada Masafumi Yagi Koji Tanase Michio Shibata
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-080, (Released:2017-04-05)
- 被引用文献数
- 7
Flower vase life of cut ornamental flowers, including carnations, is important in determining their quality and consumer preference for cut flowers. Therefore, we repeatedly crossed and selected promising offspring with long vase life for seven generations, from 1992 to 2008, in order to improve the vase life of carnation (Dianthus caryophyllus L.) flowers using conventional cross-breeding techniques. We investigated flower vase life, ethylene production at natural senescence, ethylene production after ethylene treatment (autocatalytic ethylene biosynthesis), response time to ethylene treatment (ethylene sensitivity), and flower diameter in six carnation cultivars used for initial breeding materials and a total of 123 selected lines from the first to seventh generations. Our results indicate that there was a large genetic variability in the five traits. Significant negative correlations were observed between flower vase life and ethylene production at natural senescence (r = −0.88**), between flower vase life and ethylene production after ethylene treatment (r = −0.90**), and between flower vase life and flower diameter (r = −0.92**). However, there was no correlation between flower vase life and ethylene sensitivity. Thus, the long vase life of selected carnation lines was strongly associated with a decrease in their ethylene production. Our results indicate that variation in flower vase life is not due to differences in ethylene sensitivity, but due to differences in ethylene production. Although flower size is an important floricultural trait for commercial production, crossing and selection for flower vase life resulted in a considerable reduction in flower diameter. The course of mean flower vase life over generations showed that selection was effectively made in the first to sixth generations. Repeatability for the flower vase life was roughly constant (0.3 to 0.4) in the first to sixth generations, but decreased to 0.12 in the seventh generation, suggesting a decrease in genetic variation in the seventh generation.
- 著者
- Michio Shibata
- 出版者
- Japanese Society for Plant Biotechnology
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- vol.25, no.1, pp.3-8, 2008-03-01 (Released:2008-03-15)
- 参考文献数
- 19
- 被引用文献数
- 22 36
Most of the economically important ornamental plants are cut flowers, which are produced by vegetative propagation. For many years, new varieties of ornamental plants have been produced by cross-hybridization and mutation breeding techniques, separately or in combination. Similar to mutation breeding, genetic transformation would also be a useful way of making a one-point improvement of a trait in original cultivars bred by cross-hybridization. Mutation breeding can change a dominant trait to a recessive one mostly. In other words, genetic transformation produces an “additive” one-point improvement, whereas mutation breeding produces a “subtractive” one-point improvement. Furthermore, genetic transformation can modify target traits by direct incorporation of related genes. Genetic transformation methods will be used in the near future as standard breeding tools in combination with traditional breeding methods.
- 著者
- Tawanda Elias Maguvu Yohei Higuchi Michio Shibata
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-078, (Released:2017-05-25)
- 被引用文献数
- 6
Portulaca umbraticola is an ephemeral flower that opens early in the morning and wilts in the late afternoon. Although light and temperature act as major external cues to limit the velocity of flower opening, endogenous factors regulating its timing are largely unknown. In this study, we used time lapse photography to study the effect of different photoperiods and light qualities on the flower opening rhythm of Portulaca umbraticola. When illumination was provided, flower opening was rapid and most of the flowers reached the full opening stage. In contrast, in continuous darkness (DD), progression of flower opening was similar to other treatments only during the earlier stages of flower opening; thereafter, progression was significantly slower and most flowers did not progress up to the full opening stage. A robust flower opening rhythm with a period of approximately 24 h was observed in DD for at least three days and flower opening was strongly synchronous. In contrast, continuous white (LL) and continuous red (RR) lights showed a less robust rhythm with periods of approximately 21 and 22 h, respectively, for the first two days and from the second to the third day arrhythmia developed. Continuous blue light (BB) mirrored DD, with a period of approximately 25 h. Under the different photoperiods used (20L/4D, 18L/6D, 16L/8D, 12L/12D, 8L/16D, and 4L/20D), flower opening occurred earlier at longer photoperiods in comparison with shorter photoperiods, relative to the reference point (17:00). However, when the dark period was less than 6 h, loss of synchronicity of flower opening was observed. Synchronicity of flower opening was only set when the dark period was greater than or equal to 6 h.