著者

Kenji Nashima Makoto Takeuchi Chie Moromizato Yuta Omine Moriyuki Shoda Naoya Urasaki Kazuhiko Tarora Ayaka Irei Kenta Shirasawa Masahiko Yamada Miyuki Kunihisa Chikako Nishitani Toshiya Yamamoto

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.QH-063, (Released:2023-05-31)

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The pineapple (Ananas comosus (L.) Merr.) is an economically important tropical fruit crop. In this study, we performed quantitative trait locus (QTL) analysis using 168 individuals of the F1 population of ‘Yugafu’ × ‘Yonekura’ for 15 traits: leaf color (L*, a*, b*), harvest day, crown number, slip number, stem shoot number, sucker number, fruit weight, fruit height, fruit diameter, fruit shell color, soluble solid content, acidity, and ascorbic acid content. The constructed single-nucleotide polymorphism (SNP)-based genetic linkage map consisted of a total genetic distance of 2,595 cM with 3,123 loci, including 22,330 SNPs across 25 chromosomes. QTL analysis detected 13 QTLs for 9 traits: leaf color a*, harvest day, fruit weight, fruit height, fruit diameter, fruit shell color, soluble solid content, acidity, and ascorbic acid content. The causative gene for each QTL was predicted with two genes identified as candidate genes. The AcCCD4 gene on Aco3.3C08 was the predicted causative gene for the shell color QTL, which negatively controls shell color by carotenoid degradation. The Myb domain protein-encoding gene on Aco3.3C02 was the predicted causative gene for shell color and leaf color a* QTL, which positively regulates anthocyanin accumulation. The QTL and gene information provided here contributes to future marker-assisted selection for fruit quality.

著者

Takashi Onozaki Masahiko Yamada Masafumi Yagi Koji Tanase Michio Shibata

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-080, (Released:2017-04-05)

被引用文献数

7

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Flower vase life of cut ornamental flowers, including carnations, is important in determining their quality and consumer preference for cut flowers. Therefore, we repeatedly crossed and selected promising offspring with long vase life for seven generations, from 1992 to 2008, in order to improve the vase life of carnation (Dianthus caryophyllus L.) flowers using conventional cross-breeding techniques. We investigated flower vase life, ethylene production at natural senescence, ethylene production after ethylene treatment (autocatalytic ethylene biosynthesis), response time to ethylene treatment (ethylene sensitivity), and flower diameter in six carnation cultivars used for initial breeding materials and a total of 123 selected lines from the first to seventh generations. Our results indicate that there was a large genetic variability in the five traits. Significant negative correlations were observed between flower vase life and ethylene production at natural senescence (r = −0.88**), between flower vase life and ethylene production after ethylene treatment (r = −0.90**), and between flower vase life and flower diameter (r = −0.92**). However, there was no correlation between flower vase life and ethylene sensitivity. Thus, the long vase life of selected carnation lines was strongly associated with a decrease in their ethylene production. Our results indicate that variation in flower vase life is not due to differences in ethylene sensitivity, but due to differences in ethylene production. Although flower size is an important floricultural trait for commercial production, crossing and selection for flower vase life resulted in a considerable reduction in flower diameter. The course of mean flower vase life over generations showed that selection was effectively made in the first to sixth generations. Repeatability for the flower vase life was roughly constant (0.3 to 0.4) in the first to sixth generations, but decreased to 0.12 in the seventh generation, suggesting a decrease in genetic variation in the seventh generation.

著者

Norio Takada Masahiko Yamada Sogo Nishio Yutaka Sawamura Akihiko Sato Noriyuki Onoue Toshihiro Saito

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-030, (Released:2017-02-28)

被引用文献数

3

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The pellicle of the Japanese chestnut (Castanea crenata Sieb. et Zucc.) is difficult to peel, with the exception of the recently developed ‘Porotan’, whose pellicle peelability (PP) is high and controlled by a single major gene. The objective of the present study was to identify any genetic differences in PP in Japanese chestnuts with difficult-peeling pellicles. We detected a significant genetic difference in PP (evaluated as the nut surface area that peeled away without scraping with a hand knife after deep frying in cooking oil) among five Japanese chestnut cultivars grown in Tsukuba, Japan, using two trees in 2 years, and among three cultivars grown in five locations using a single tree in 1 year. We evaluated the PP of 32 Japanese chestnut cultivars/selections and one wild clone (Shibaguri-37) using a single tree in 3 years to quantify the variation. The broad-sense heritability of mean values over the 3 years was estimated as 0.67. Shibaguri-37 had the highest PP. The suggested new genes controlling the variation in PP have high potential in terms of breeding strategy for easy-peeling pellicles as an alternative to the major gene of ‘Porotan’, the use of which is likely to result in inbreeding.