- 著者
- Katsuhiko Sumitomo Kenta Shirasawa Sachiko N. Isobe Hideki Hirakawa Akiho Harata Masato Kawabe Masafumi Yagi Masaaki Osaka Miyuki Kunihisa Fumiya Taniguchi
- 出版者
- Japanese Society of Breeding
- 雑誌
- Breeding Science (ISSN:13447610)
- 巻号頁・発行日
- pp.20063, (Released:2021-03-25)
- 被引用文献数
- 6
White rust caused by Puccinia horiana Henn. adversely affects chrysanthemum (Chrysanthemum morifolium Ramat.) production. The breeding of resistant varieties is effective in controlling the disease. Here we aimed to develop DNA markers for the strong resistance to P. horiana. We conducted a linkage analysis based on the genome-wide association study (GWAS) method. We employed a biparental population for the GWAS, wherein the single nucleotide polymorphism (SNP) allele frequency could be predicted. The population was derived from crosses between a strong resistant “Southern Pegasus” and a susceptible line. The GWAS used simplex and double-simplex SNP markers selected out of SNP candidates mined from ddRAD-Seq data of an F1 biparental population. These F1 individuals segregated in a 1:1 ratio of resistant to susceptible. Twenty-one simplex SNPs were significantly associated with P. horiana resistance in “Southern Pegasus” and generated one linkage group. These results show the presence of a single resistance gene in “Southern Pegasus”. We identified the nearest SNP marker located 2.2 cM from P. horiana resistance locus and demonstrated this SNP marker-resistance link using an independent population. This is the first report of an effective DNA marker linked to a gene for P. horiana resistance in chrysanthemum.
- 著者
- Takamitsu Waki Masaharu Kodama Midori Akutsu Kiyoshi Namai Masayuki Iigo Takeshi Kurokura Toshiya Yamamoto Kenji Nashima Masayoshi Nakayama Masafumi Yagi
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-096, (Released:2017-09-29)
- 被引用文献数
- 1 13
Double flower and hortensia (mophead) hydrangea (Hydrangea macrophylla (Thunb.) Ser.) traits are recessively inherited. Cross breeding of these traits in hydrangea is difficult because it takes about two years from crossing to flowering. In this study, we aimed to obtain DNA linkage markers that would allow accelerated selection of these traits. We used next-generation sequencing to comprehensively collect DNA sequences from the ‘Kirakiraboshi’ with a double flower and lacecap inflorescence and the ‘Frau Yoshimi’ with a single flower and hortensia inflorescence, and designed simple sequence repeat (SSR) primer pairs for map construction. We screened 768 SSR primer pairs in 93 F2 progeny derived from ‘Kirakiraboshi’ and ‘Frau Yoshimi’. We identified 147 loci, which were expanded to 18 linkage groups with a total map length of 980 cM. Linkage analysis identified that both the double flower trait from ‘Kirakiraboshi’ (dKira) and the hortensia trait from ‘Frau Yoshimi’ (hFrau) were located on linkage group KF_4. Detailed linkage analysis using 351 F2 progeny revealed a 34.8 cM map length between the two loci and identified two tightly linked SSR markers, STAB045 for dKira and HS071 for hFrau. Genetic analysis suggested that double flower and hortensia traits are each controlled by a single recessive gene. Together, the linkage map, SSR markers, and genetic information obtained in this study will be useful for future hydrangea breeding.
- 著者
- Takashi Onozaki Masahiko Yamada Masafumi Yagi Koji Tanase Michio Shibata
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-080, (Released:2017-04-05)
- 被引用文献数
- 7
Flower vase life of cut ornamental flowers, including carnations, is important in determining their quality and consumer preference for cut flowers. Therefore, we repeatedly crossed and selected promising offspring with long vase life for seven generations, from 1992 to 2008, in order to improve the vase life of carnation (Dianthus caryophyllus L.) flowers using conventional cross-breeding techniques. We investigated flower vase life, ethylene production at natural senescence, ethylene production after ethylene treatment (autocatalytic ethylene biosynthesis), response time to ethylene treatment (ethylene sensitivity), and flower diameter in six carnation cultivars used for initial breeding materials and a total of 123 selected lines from the first to seventh generations. Our results indicate that there was a large genetic variability in the five traits. Significant negative correlations were observed between flower vase life and ethylene production at natural senescence (r = −0.88**), between flower vase life and ethylene production after ethylene treatment (r = −0.90**), and between flower vase life and flower diameter (r = −0.92**). However, there was no correlation between flower vase life and ethylene sensitivity. Thus, the long vase life of selected carnation lines was strongly associated with a decrease in their ethylene production. Our results indicate that variation in flower vase life is not due to differences in ethylene sensitivity, but due to differences in ethylene production. Although flower size is an important floricultural trait for commercial production, crossing and selection for flower vase life resulted in a considerable reduction in flower diameter. The course of mean flower vase life over generations showed that selection was effectively made in the first to sixth generations. Repeatability for the flower vase life was roughly constant (0.3 to 0.4) in the first to sixth generations, but decreased to 0.12 in the seventh generation, suggesting a decrease in genetic variation in the seventh generation.