著者

María del Carmen Marín Alexander L. Jaffe Patrick T. West Masae Konno Jillian F. Banfield Keiichi Inoue

出版者

The Biophysical Society of Japan

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

pp.e201023, (Released:2023-03-08)

被引用文献数

1

---

Microbial rhodopsins are photoreceptive transmembrane proteins that transport ions or regulate other intracellular biological processes. Recent genomic and metagenomic analyses found many microbial rhodopsins with unique sequences distinct from known ones. Functional characterization of these new types of microbial rhodopsins is expected to expand our understanding of their physiological roles. Here, we found microbial rhodopsins having a DSE motif in the third transmembrane helix from members of the Actinobacteria. Although the expressed proteins exhibited blue–green light absorption, either no or extremely small outward H+ pump activity was observed. The turnover rate of the photocycle reaction of the purified proteins was extremely slow compared to typical H+ pumps, suggesting these rhodopsins would work as photosensors or H+ pumps whose activities are enhanced by an unknown regulatory system in the hosts. The discovery of this rhodopsin group with the unique motif and functionality expands our understanding of the biological role of microbial rhodopsins.

著者

井上 圭一 今野 雅恵 川﨑 佑真 María del Carmen MARÍN

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.63, no.5, pp.257-260, 2023 (Released:2023-11-25)

参考文献数

16

---

シゾロドプシンは真核生物の最終共通祖先に最も近縁な現生種のアスガルドアーキアから見付かった光駆動型内向きH+ポンプロドプシンであり,アスガルドアーキアが光を使って生きている可能性を示唆するものである.H+輸送機構など多くの点でシゾロドプシンは他のロドプシンと異なる.その最新の研究を解説する.