著者

Darunmas Sankhuan Meiqiao Ji Sota Takanashi Yuto Imamura Shoichi Sato Kanyaratt Supaibulwatana Masahiro Otani Masaru Nakano

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.39, no.2, pp.205-208, 2022-06-25 (Released:2022-06-25)

参考文献数

14

被引用文献数

2

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LEAFY (LFY), which encodes a plant-specific transcription factor, plays an important role in the transition from vegetative to reproductive development. Ectopic expression of LFY has been reported to induce dwarfism and early flowering in some model plants. In order to examine the possibility of using LFY for molecular breeding of ornamental plants, we produced and characterized transgenic plants ectopically expressing LFY from Arabidopsis thaliana (AtLFY) in the liliaceous ornamental plant Tricyrtis sp. Nine independent transgenic plants have been obtained, and all of them exhibited dwarf phenotypes compared with the vector control. These transgenic plants could be classified into three types according to the degree of dwarfism: one showed an extreamly dwarf phenotype with smaller leaves (Type I); two showed moderately dwarf phenotypes (Type II); and six showed slightly dwarf phenotypes (Type III). All of Type I, Type II and Type III transgenic plants produced flower buds 1–3 weeks earlier than the vector control. Vector control and Type III transgenic plants produced 1–4 apical flower buds, whereas Type I and Type II transgenic plants produced only a single apical flower bud. Type I and Type II transgenic plants often produced non-fully-opened flowers. Quantitative real-time reverse transcription-polymerase chain reaction analysis showed that the AtLFY expression level generally correlated with the degree of dwarfism. These results indicate that morphological alterations observed in the transgenic plants was induced by ectopic expression of AtLFY. Lower levels of ectopic expression of LFY may be valuable for producing dwarf and early flowering ornamental plants.

著者

Kana Miura Mutsumi Nakada Shosei Kubota Shusei Sato Soichiro Nagano Akie Kobayashi Mika Teranishi Masaru Nakano Akira Kanno

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.UTD-036, (Released:2018-12-26)

被引用文献数

3

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The modified ABC model explains the floral morphology of many monocots, such as the lily and tulip, in which the perianth consists of two layers of almost identical petaloid tepals. According to the modified ABC model, B-class genes are expressed in two perianth whorls, inducing the petaloid structure in both whorls 1 and 2. In this study, we analyzed the expression and function of the B-class genes in the grape hyacinth (Muscari armeniacum). We isolated two DEFICIENS (DEF)-like genes (MaDEF1 and MaDEF2) and three GLOBOSA (GLO)-like genes (MaGLOA1, MaGLOA2, and MaGLOB) from M. armeniacum using rapid amplification of cDNA ends (RACE). Expression analysis showed that MaDEF1 and MaDEF2 were expressed in whorls 1, 2, and 3, whereas MaGLOA1, MaGLOA2, and MaGLOB were expressed in all four whorls. These results support the modified ABC model in M. armeniacum. Overexpression of MaGLOA1 and MaGLOB in Arabidopsis thaliana resulted in a morphological change of sepals to petaloid structures in whorl 1, indicating that the function of these genes is similar that of the B-class orthologs PISTILLATA and GLO in A. thaliana and Antirrhinum majus, respectively. In addition, yeast two-hybrid assays revealed strong protein–protein interactions between MaDEF1 and MaGLOA1, suggesting that MaDEF1–MaGLOA1 is likely to have the main B-function in M. armeniacum. These data support the modified ABC model in M. armeniacum.

著者

Takeomi IMANI Masaru NAKANO

出版者

International Association of P2M

雑誌

国際P2M学会研究発表大会予稿集 (ISSN:24320382)

巻号頁・発行日

pp.49-64, 2018 (Released:2018-04-16)

参考文献数

52

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Conventional, IT system development organizations have selectively utilized the two techniques: plan-driven method in which requirements are defined and baselined in early phase of the project and agile method which is based on iterative development. Recently, the research articles that reported a hybrid approach that uses plan-driven and agile method together have been on the rise. However, few theoretical studies have investigated the contextual conditions that illustrates a better fit for hybrid approach, called effective area. This research is aimed to provide an analysis model of hybrid approach by evaluating project characteristics and iteration development strategy. Our model and numerical simulations present that a hybrid approach should be fit with wider context of project characteristics than the agile method. By investigating the effective area with multiple parameters, this research would contribute to a growing literature of hybrid approach and a methodology for project manager or project management office to choose hybrid approach appropriately.