著者

Michiko HIGASHI Saori TAHARABARU Yushi U. ADACHI Maiko SATOMOTO Takahiro TAMURA Naoyuki MATSUDA Aiji SATO-BOKU Masahiro OKUDA

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.23-0010, (Released:2023-06-02)

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Administration in a lipid emulsion can modify the pharmacodynamics of drugs via a process known as lipid resuscitation. However, the detailed mechanism remains unclear. We studied the volume and another pharmacodynamic effect, the lipid sink, using propofol and thiamylal. Male adult mice (ddY) were intravenously administered 10 mL/kg propofol or thiamylal diluted with physiological saline, 10% soybean oil, or 20% soybean oil. The 50% effective dose (ED50) for achieving hypnosis was calculated using probit analysis. To investigate the volume effect, 0, 10, or 20 mL/kg of saline or soybean oil was administered, either simultaneously or beforehand. Next, a two- or three-fold dose of the anesthetics was administered and the durations of anesthesia were measured. Finally, at 30 s after the first injection, supplemental soybean oil was administered. The mean (± standard error) ED50 values of propofol and thiamylal were 5.79 mg/kg (0.61) and 8.83 mg/kg (0.84), respectively. Lipid dilution increased the ED50 values of both anesthetics. After injection of a dose two-fold the ED50 value, the respective mean (± standard deviation) durations of anesthesia were 125 ± 35 s and 102 ± 38 s. Supplemental administration of soybean oil significantly shortened the duration of anesthesia of propofol, but not that of thiamylal. The results indicate that administration of a lipid emulsion vitiated the anesthetic effect of propofol by reducing the non-emulsified free fraction in the aqueous phase, which may elucidate the lipid resuscitation likely caused by the lipid sink mechanism.

著者

木原 資裕 皆川 直凡 立岡 裕士 藪下 克彦 内藤 隆 田村 隆宏 南 隆尚 町田 哲 久米 禎子 眞野 美穂 畠山 輝雄 小倉 正義 Motohiro KIHARA Naohiro MINAGAWA Yuuzi TATUOKA Katsuhiko YABUSHITA Takashi NAITO Takahiro TAMURA Takahisa MINAMI Tetsu MACHIDA Teiko KUME Miho MANO Teruo HATAKEYAMA Masayoshi OGURA

出版者

鳴門教育大学

雑誌

鳴門教育大学研究紀要 = Research bulletin of Naruto University of Education (ISSN:18807194)

巻号頁・発行日

vol.36, pp.334-348, 2021-03-10

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Naruto University of Education offers an undergraduate class called "Awa (Tokushima) Studies", which is taught by twelve instructors whose specialties span history, geography, psychology, art, physical education, and linguistics. In this class, after studying the background and history of Ohenro, a pilgrimage route in Shikoku, in classroom lectures, the students and the instructors walk a part of the pilgrimage route on a one-night-two-day trip, in which interdisciplinary studies are practiced. This class is an ideal opportunity for teacher training and therefore can serve to strategically promote Naruto University of Education as a unique university in offering such a class.