著者

青木 嵩 Takashi Aoki

雑誌

KGPS review:Kwansei Gakuin policy studies review

巻号頁・発行日

no.25, pp.5-14, 2018-03-31

著者

Akito Tsuchiya Aki Nishihara Ayumi Saeki Yuki Teru Takashi Aoki Tomoya Kono Masahiro Sakai Jun-ichi Hikima

出版者

The Japanese Society of Fish Pathology

雑誌

魚病研究 (ISSN:0388788X)

巻号頁・発行日

vol.55, no.1, pp.18-21, 2020-03-15 (Released:2020-04-02)

参考文献数

25

被引用文献数

1

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Pseudotuberculosis pathogen, Photobacterium damselae subsp. piscicida (Pdp) shows some differences in virulence between Japanese and US strains. ​In this study, we analyzed plasmid DNAs derived from Japanese and US strains of Pdp, and found two homologous regions in these plasmids, pOT-51443-4/p91-197-1 and pOT-51443-1/p91-197-2. ​All genes in the p91-197-1 from US strain were observed in the pOT-51443-4 from Japanese strain. ​This event might have occurred during transmission of the US plasmid to Japanese strain. ​Inhibitor of vertebrate lysozyme (ivy), a known pathogenicity factor was encoded in the homologous region of pOT-51443-1/p91-197-2. ​Thus, the genomic region containing ivy may have been horizontally transmitted between the two Pdp strains.

著者

Yusuke Kamiyoshihara Takuya Nakamura Yasuharu Itagaki Shinichi Asada Takashi Aoki Shinji Mizuno Keiichi Watanabe Hiroaki Inoue Akira Tateishi

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-133, (Released:2018-02-01)

被引用文献数

2

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Actinidin is a major protein contained in kiwifruit (Actinidia spp.). While uptake of actinidin is beneficial to help gastric protein digestion with cysteine protease activity, the protein is also recognized as a major elicitor of allergy which can induce tingling in the oral cavity and occasionally severe anaphylactic reactions. Given that consumption of fresh kiwifruit has increased globally, development of Actinidia cultivars with lower level of actinidin is required to reduce the risk of allergenicity. In the present study, we examined variations in the actinidin level in Actinidia varieties. Among several varieties having trace amounts of actinidin, A. chinensis ‘Kohi’ was targeted to be analyzed for the molecular basis for the phenotype. ‘Kohi’ had below the detectable transcript level of Act1a, a critical gene for actinidin level. The upstream region of Act1a in ‘Kohi’ constituted different sequences from that of A. deliciosa ‘Hayward’, which has an active promoter for high expression of Act1a. The ‘Kohi’ sequence in the diverged region (upstream from −873 b) was rich in cytosine residues methylated at a higher level than in ‘Hayward’. Our data suggest the possibility of novel epigenetic regulation to reduce the actinidin level. The molecular mechanism for the phenotype in ‘Kohi’ was differentiated from ‘Hort16A’, a globally popular cultivar with a low level of actinidin. This cultivar could be a choice as a genetic resource in breeding to develop cultivars with controlled actinidin levels.