- 著者
- Naoki Sakane Yoko Asano Tomoko Kawamura Tomohiro Takatani Yasuhiro Kohama Kazutake Tsujikawa Hiroshi Yamamoto
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- Biological and Pharmaceutical Bulletin (ISSN:09186158)
- 巻号頁・発行日
- vol.27, no.12, pp.2014-2020, 2004 (Released:2004-12-01)
- 参考文献数
- 29
- 被引用文献数
- 1 2 3
Fetal liver (FL) hematopoiesis is thought to be important for expanding the cell number during ontogeny. In order to investigate the cellular interaction molecules among FL stromal and hematopoietic cells, we established a monoclonal antibody, Ndk-10, that reacts with FL stromal cells but not with dish non-adherent cells. When Ndk-10 was added to an FL stromal and hematopoietic cell-coculture, it inhibited the survival of c-kit+ cells. The inhibitory activity of Ndk-10 was also observed in the fetal liver organ culture. The Ndk-10 recognized a 150 kD molecule in the adherent cells of FL and kidney, and the N-terminal amino acid sequence was identical to that of mouse aminopeptidase N/CD13. The peptidase activity of CD13 was inhibited by Ndk-10, and addition of its specific inhibitor resulted in the same inhibitory activity as Ndk-10. We propose that aminopeptidase N/CD13 is a critical molecule that regulates the survival of c-kit+ cells in the FL microenvironment.
- 著者
- Tomohiro TAKATANI Hiroshi AKAEDA Teruo KAKU Masahide MIYAMOTO Hirohiko MUKAI Tamao NOGUCHI
- 出版者
- Japanese Society for Food Hygiene and Safety
- 雑誌
- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) (ISSN:00156426)
- 巻号頁・発行日
- vol.39, no.4, pp.292-295_1, 1998-08-05 (Released:2009-12-11)
- 参考文献数
- 11
- 被引用文献数
- 5 5
最近, 九州地域ではこれまで例がほとんどない Gymnodinium catenatum により二枚貝が毒化している. 1998年1月下旬, 熊本県天草の宮野河内湾で天然マガキが麻痺性貝毒 (PSP) により毒化した. 同湾から, 1996年の大分県蒲江に引き続き, G. catenatum が密度308細胞/mLで確認された. カキの毒性値は3.0~263MU/gを示し, HPLC分析の結果, その毒成分はカキ, プランクトンともにC1 (PX1), C2 (PX2) を主成分としていた. この結果, カキは食物連鎖を通して G. catenatum により毒化したと結論した.