著者

Yuko Maki Hiroshi Soejima Toru Kitamura Tamizi Sugiyama Takeo Sato Masaaki K. Watahiki Junji Yamaguchi

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.1, pp.9-16, 2021-03-25 (Released:2021-03-25)

参考文献数

32

被引用文献数

9

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Bokashi fertilizer, an organic fertilizer made of plant residue, has been used in Japan not only to fertilize plants but to regulate their growth. Lactic acid bacteria have been found to play an important role in the fermentation process of Bokashi, but the relationship between these bacteria and plant growth activity has not been clarified. Using the adzuki rooting assay, this study identified 3-phenyllactic acid (PLA) produced by lactic acid bacteria as a root promoting compound in Bokashi. PLA showed synergistic effect with tryptophan, but no stem elongation activity. Lactic acid bacteria produced equal quantities of the L- and D-forms of PLA, which have similar root promoting activity. PLA did not significantly affect the amount of endogenous indole-3-acetic acid (IAA), although the chemical structure of PLA is highly similar to that of L-2-aminooxy-3-phenypropionic acid (L-AOPP), which inhibits IAA biosynthesis. These results indicate that the root promoting activity of PLA is not simply due to its increase in the amount of active auxin.

著者

柳本 卓 北村 徹 TAKASHI YANAGIMOTO TORU KITAMURA 北海道区水産研究所 日本エス・ユー・エス株式会社 Groundjish Biology Section Subarctic Fisheries Resources Division Hokkaido National Fisheries Research Institute Laboratory of Environmental Biology Japan NUS Co. Ltd.

出版者

The Japanese Society of Fisheries Science

雑誌

日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries (ISSN:00215392)

巻号頁・発行日

vol.68, no.6, pp.893-899, 2002-11-15

参考文献数

21

被引用文献数

7 4

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タラ科3種(スケトウダラ,マダラ,コマイ)の成魚の筋肉から抽出した粗DNAを用いPCR法にて増幅したシトクロームb領域の塩基配列を決定した。塩基配列からDpnII,HaeIII, RsaIおよびTaqIの4種類の制限酵素により,タラ科3種の種判別ができることが明らかになった。また,種特異的なプライマーを設計して,増幅した断片の長さの違いで3種を識別する方法を確立した。これらの手法により,形態的な差異だけでは困難なタラ科仔稚魚の種判別が可能になった。Mitochondrial DNA sequences of the cytochrome b gene (1161bp) were obtained from three gadoid species (Theragra chalcogramma, Gadus macrocephalus, and Eleginus gracilis) near Hokkaido, Japan. Four restriction enzymes (DpnII, HaeIII, RsaI and TaqI) were diagnostic to identify these three species. Species specific internal primers were designed based upon the characteristic nucleotide substitutions in each species, which allowed discrimination among these three species by amplicon size.