著者

Gentaro Shigita Tran Phuong Dung Mst. Naznin Pervin Thanh-Thuy Duong Odirich Nnennaya Imoh Yuki Monden Hidetaka Nishida Katsunori Tanaka Mitsuhiro Sugiyama Yoichi Kawazu Norihiko Tomooka Kenji Kato

出版者

Japanese Society of Breeding

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

pp.22071, (Released:2023-06-15)

被引用文献数

1

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Numerous genetic resources of major crops have been introduced from around the world and deposited in Japanese National Agriculture and Food Research Organization (NARO) Genebank. Understanding their ge‍netic variation and selecting a representative subset (“core collection”) are essential for optimal management and efficient use of genetic resources. In this study, we conducted genotyping-by-sequencing (GBS) to characterize the genetic relationships and population structure in 755 accessions of melon genetic resources. The GBS identified 39,324 single-nucleotide polymorphisms (SNPs) that are distributed throughout the melon genome with high density (one SNP/10.6 kb). The phylogenetic relationships and population structure inferred using this SNP dataset are highly associated with the cytoplasm type and geographical origin. Our results strongly support the recent hypothesis that cultivated melon was established in Africa and India through multiple independent domestication events. Finally, we constructed a World Melon Core Collection that covers at least 82% of the genetic diversity and has a wide range of geographical origins and fruit mor‍phology. The genome-wide SNP dataset, phylogenetic relationships, population structure, and the core collection provided in this study should largely contribute to genetic research, breeding, and genetic resource preservation in melon.

著者

Yoichi Kawazu Shunsuke Imanishi Hirotaka Yamaguchi Akio Ohyama Tsukasa Nunome Koji Miyatake Hiroyuki Fukuoka

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.UTD-022, (Released:2018-11-03)

被引用文献数

1

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The Cauliflower mosaic virus 35S promoter (P35S) induces transgene expression with insufficient activity and stability in some plant species, including lettuce. To develop a system to provide sufficient gene expression, a polyubiquitin promoter (PLsUbi) and terminator (TLsUbi) were isolated from lettuce, and this system was functionally compared with the conventional P35S-NOS terminator (P35S-Tnos) system by using a β-glucuronidase (GUS) reporter gene. In transgenic Arabidopsis, PLsUbi induced higher GUS activity than P35S, and the PLsUbi-TLsUbi combination induced higher GUS activity compared with the PLsUbi-Tnos combination, suggesting that the polyubiquitin terminator promotes transgene expression in concert with PLsUbi. The PLsUbi-TLsUbi combination induced less accumulation of GUS mRNA but > 10-fold higher GUS enzyme activity than the P35S-Tnos combination, suggesting that the PLsUbi-TLsUbi combination translationally promoted GUS expression in Arabidopsis. In transgenic lettuce, PLsUbi-TLsUbi transcrip­tionally and translationally promoted GUS expression, inducing approximately 16-fold-higher accumulation of GUS mRNA and > 800-fold-higher GUS enzyme activity compared with those induced by P35S-Tnos. Bisulfite sequencing methylation analysis of the introduced promoter sequences indicated that, for PLsUbi, the mean percentage of methylated cytosines in lettuce was 3.5 times that in Arabidopsis. For P35S, the mean percentage of methylated cytosines in lettuce was > 10 times that in Arabidopsis, and this methylation may be a major reason underlying the transcriptional inactivation of P35S in lettuce. Together, our results indicate that PLsUbi-TLsUbi promotes transgene expression in lettuce and Arabidopsis and may have broad applications in genetic engineering of additional plant species.