- 著者
- Rihito Takisawa Takayuki Maruyama Tetsuya Nakazaki Keiko Kataoka Hiroki Saito Sota Koeda Tsukasa Nunome Hiroyuki Fukuoka Akira Kitajima
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-042, (Released:2017-03-03)
- 被引用文献数
- 20
Parthenocarpy is a trait where fruit set and growth are triggered without pollination and fertilization. In the tomato (Solanum lycopersicum L.), this trait is considered attractive as it reduces the cost and labor requirements for fruit setting. In this study, we investigated the inheritance of parthenocarpy in ‘MPK-1’—a parthenocarpic tomato cultivar derived from a cross between a variant from a self-fertilization posterity of ‘Severianin’, which exhibited strong parthenocarpy and a non-parthenocarpic cultivar. It was reported that ‘MPK-1’ contains a pat-2 gene because ‘Severianin’ which has a pat-2 gene is its only parthenocarpic ancestor. However, we found that parthenocarpy in ‘MPK-1’ is controlled by a novel parthenocarpic gene, not pat-2. This novel gene, which was designated as Pat-k, is semi-dominant and located on chromosome 1. We also showed that the size of the parthenocarpic fruit of ‘MPK-1’ is similar to that of the pollinated fruit at maturity. Thus, ‘MPK-1’ may be used as a new parthenocarpic resource for breeding.
- 著者
- Tong Ly Hiroyuki Fukuoka Asami Otaka Atsushi Hoshino Shigeru Iida Eiji Nitasaka Nobuyoshi Watanabe Tsutomu Kuboyama
- 出版者
- Japanese Society of Breeding
- 雑誌
- Breeding Science (ISSN:13447610)
- 巻号頁・発行日
- vol.62, no.1, pp.99-104, 2012 (Released:2012-03-20)
- 参考文献数
- 26
- 被引用文献数
- 5 5
Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.
- 著者
- Yoichi Kawazu Shunsuke Imanishi Hirotaka Yamaguchi Akio Ohyama Tsukasa Nunome Koji Miyatake Hiroyuki Fukuoka
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.UTD-022, (Released:2018-11-03)
- 被引用文献数
- 1
The Cauliflower mosaic virus 35S promoter (P35S) induces transgene expression with insufficient activity and stability in some plant species, including lettuce. To develop a system to provide sufficient gene expression, a polyubiquitin promoter (PLsUbi) and terminator (TLsUbi) were isolated from lettuce, and this system was functionally compared with the conventional P35S-NOS terminator (P35S-Tnos) system by using a β-glucuronidase (GUS) reporter gene. In transgenic Arabidopsis, PLsUbi induced higher GUS activity than P35S, and the PLsUbi-TLsUbi combination induced higher GUS activity compared with the PLsUbi-Tnos combination, suggesting that the polyubiquitin terminator promotes transgene expression in concert with PLsUbi. The PLsUbi-TLsUbi combination induced less accumulation of GUS mRNA but > 10-fold higher GUS enzyme activity than the P35S-Tnos combination, suggesting that the PLsUbi-TLsUbi combination translationally promoted GUS expression in Arabidopsis. In transgenic lettuce, PLsUbi-TLsUbi transcriptionally and translationally promoted GUS expression, inducing approximately 16-fold-higher accumulation of GUS mRNA and > 800-fold-higher GUS enzyme activity compared with those induced by P35S-Tnos. Bisulfite sequencing methylation analysis of the introduced promoter sequences indicated that, for PLsUbi, the mean percentage of methylated cytosines in lettuce was 3.5 times that in Arabidopsis. For P35S, the mean percentage of methylated cytosines in lettuce was > 10 times that in Arabidopsis, and this methylation may be a major reason underlying the transcriptional inactivation of P35S in lettuce. Together, our results indicate that PLsUbi-TLsUbi promotes transgene expression in lettuce and Arabidopsis and may have broad applications in genetic engineering of additional plant species.