- 著者
- Masafumi Seki Naruhiko Ikari Seigo Yamamoto Yuhki Yamagata Kosuke Kosai Katsunori Yanagihara Tomoyuki Kakugawa Shintaro Kurihara Koichi Izumikawa Yoshitsugu Miyazaki Yasuhito Higashiyama Yoichi Hirakata Takayoshi Tashiro Shigeru Kohno
- 出版者
- 一般社団法人 日本内科学会
- 雑誌
- Internal Medicine (ISSN:09182918)
- 巻号頁・発行日
- vol.45, no.22, pp.1323-1326, 2006 (Released:2006-12-15)
- 参考文献数
- 22
- 被引用文献数
- 11 24 11
A 77 years old woman who had a bite with eschar on her left arm, was admitted to emergency ward in our hospital, because of high fever, severe malaise, skin eruption, and consciousness disturbance beginning 5 days previously. She was diagnosed as Japanese spotted fever by seropositive of Rickettsia japonica (R. japonica) antibody, and successfully treated with fluoroquinolone, after minocycline hydrochloride had been proven ineffective. R. japonica-specific DNA was detected by PCR from the tick: Haemaphysalis hystricis larvae collected from a mountainous location in Fukuoka, Japan where the patient had been bitten.
- 著者
- Madoka Tateno Takashi Umeyama Tatsuya Inukai Shogo Takatsuka Yasutaka Hoshino Satoshi Yamagoe Somay Yamagata Murayama Keiko Ishino Yoshitsugu Miyazaki
- 出版者
- The Japanese Society for Medical Mycology
- 雑誌
- Medical Mycology Journal (ISSN:21856486)
- 巻号頁・発行日
- pp.21-00024, (Released:2022-02-16)
- 参考文献数
- 29
- 被引用文献数
- 4
Aspergillus lentulus was first reported in 2005 as a cryptic species of Aspergillus fumigatus, and since then, its resistance to azole drugs and the high mortality rate of infected individuals have emerged as problems. Although it has been reported that P450 14-α sterol demethylase (Cyp51) is involved in azole resistance in A. lentulus, the specific resistance mechanism has not been elucidated. In this study, we successfully introduced the entire A. fumigatus cyp51A gene into the cyp51A locus in A. lentulus using the CRISPR/Cas9 genome-editing system. The A. lentulus strains harboring A. fumigatus cyp51A showed reduced minimum inhibitory concentrations for itraconazole and voriconazole compared with those of the parent strain. This finding suggests that Cyp51A is involved in azole resistance in A. lentulus and may contribute to the elucidation of the mechanism of resistance to azole drugs via Cyp51A and to the development of new antifungal drugs. In addition, our successful application of the CRISPR/Cas9 system to A. lentulus opens the door to examination of other gene functions in this fungus.
- 著者
- Koichi Izumikawa Hiroshi Kakeya Fumikazu Sakai Kazutoshi Shibuya Takashi Sugita Takahiro Takazono Tohru Takata Masato Tashiro Katsuji Teruya Shigeki Nakamura Hiromitsu Noguchi Masataro Hiruma Koichi Makimura Taiga Miyazaki Yoshitsugu Miyazaki Yuka Yamagishi Koichiro Yoshida Akira Watanabe
- 出版者
- The Japanese Society for Medical Mycology
- 雑誌
- Medical Mycology Journal (ISSN:21856486)
- 巻号頁・発行日
- vol.61, no.4, pp.61-89, 2020 (Released:2020-11-30)
- 参考文献数
- 39
- 被引用文献数
- 7
- 著者
- Keigo Ueno Nao Yanagihara Kiminori Shimizu Yoshitsugu Miyazaki
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Biological and Pharmaceutical Bulletin (ISSN:09186158)
- 巻号頁・発行日
- vol.43, no.2, pp.230-239, 2020-02-01 (Released:2020-02-01)
- 参考文献数
- 102
- 被引用文献数
- 24
Cryptococcosis is a potentially lethal disease caused by fungal pathogens including Cryptococcus neoformans and Cryptococcus gattii species complex. These fungal pathogens live in the environment and are associated with certain tree species and bird droppings. This infectious disease is not contagious, and healthy individuals may contract cryptococcal infections by inhaling the airborne pathogens from the environment. Although cleaning a contaminated environment is a feasible approach to control environmental fungal pathogens, prophylactic immunization is also considered a promising method to regulate cryptococcal infections. We review the history of the development of cryptococcal vaccines, vaccine components, and the various forms of immune memory induced by cryptococcal vaccines.