著者

Takuya Wada Yuji Noguchi Sachiko Isobe Miyuki Kunihisa Takayuki Sueyoshi Katsumi Shimomura

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-142, (Released:2017-01-14)

被引用文献数

11

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A strawberry core collection was established based on simple sequence repeat and cleavage amplified polymorphic sequence marker polymorphisms in 119 strawberry cultivars using the “PowerCore” program. The core collection consisted of 19 cultivars. The correlation coefficients for the diversity index were significant between the core collection cultivars and all cultivars. Allele frequencies of each marker allele were not significantly different between the core collection cultivars and all cultivars according to Fisher’s exact test. Cluster analysis indicated that the selected core collection cultivars evenly distributed throughout the multiple clusters and principle component analysis clearly showed major principle components of core collection cultivars distributed widely among those of all cultivars. Furthermore, core collection cultivars tended to harbor minor alleles. These results demonstrated that the core collection cultivars were suitably selected in terms of reflecting the genetic diversity of all strawberry cultivars.

著者

Misaki Ishibashi Shunji Okochi Kazuyoshi Sone Yuji Noguchi Yuichi Uno

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.UTD-051, (Released:2019-03-07)

被引用文献数

5

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Strawberry (Fragaria × ananassa) contains a major allergen, Fra a 1, which causes oral allergic syndrome. Fra a 1 is a PR-10 homolog that is regulated by environmental conditions. The allergenicity of fruit caused by Fra a 1 may depend on the genotype or growing conditions. We analyzed the Fra a 1.01 transcript levels and Fra a 1.01 protein levels in strawberry fruits of several genotypes across all seasons. In the preliminary rough screening, we selected the line WH1 bearing white fruit and the red-fruited cultivar ‘Akihime’. Under the same environmental conditions, there was no significant difference in Fra a 1.01 levels between the two cultivars over several months, suggesting that receptacle color was not indicative of allergenicity caused by Fra a 1.01. Fruits cultivated under the same environmental conditions should be used for comparisons of the allergenicity among genotypes. Both ‘Akihime’ and WH1 accumulated significantly higher levels of Fra a 1.01 protein in winter than in spring. We investigated the effects of irradiation and low temperature as environmental factors controlling the accumulation of Fra a 1.01 in winter. A shading treatment on fruit did not significantly affect Fra a 1.01 protein accumulation in strawberry fruits. Regarding variations over time, the Fra a 1.01 protein content was higher in fruits harvested at midnight in January than in those harvested at other times and in other months. These findings suggested that the Fra a 1.01 protein accumulates in response to environmental factors such as cold stress.