著者

Tomonari Hirano Yuka Matsuyama Anna Hanada Yoriko Hayashi Tomoko Abe Hisato Kunitake

出版者

Japan Mendel Society, International Society of Cytology

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.86, no.4, pp.311-315, 2021-12-25 (Released:2021-12-24)

参考文献数

23

被引用文献数

2

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Heavy-ion beams have been applied as effective mutagens to various plant materials. Pollen has been used as material for mutant induction and genetic analysis. However, our knowledge of the DNA damage response of plant male gametes remains limited. In the present study, we irradiated Cyrtanthus mackenii pollen with an argon ion beam, which induced complex DNA damage, and investigated the DNA damage response of male gametes during pollen tube growth. Male gametes derived from the irradiated pollen grains were isolated from pollen tubes after 12 and 24 h of culture and subjected to cell cycle analysis. After 12 h of culture, the irradiated generative cells were frequently arrested at metaphase during pollen mitosis II (PMII), and the proportion of metaphase cells increased with increasing absorbed dose. These results suggest that the genomic lesions induced by the argon ion beam caused spindle assembly checkpoint (SAC)-dependent arrest. After 24 h of culture, the irradiated male gametes completed PMII, albeit forming sperm cells with abnormalities in chromosome separation, and chromosomal bridges were often formed between these cells. Moreover, phosphorylated H2AX foci, an indicator of DNA double-strand breaks, were detected in the irradiated male gametes after 24 h of culture, regardless of passing through the SAC. Taken together, these results indicate that male gametes activate functions to cope with radiation-induced complex DNA damage during pollen tube growth.

著者

伴 みずほ 山科 舞子 嶋崎 美香 松山 由佳 宮本 有香 高澤 弘明 福田 正子 Mizuho Ban Maiko Yamashina Mika Shimazaki Yuka Matsuyama Yuka Miyamoto Hiroaki Takazawa Masako Fukuda 京都短期大学生活福祉 京都短期大学生活福祉:(現)たじま農業協同組合 京都短期大学生活福祉:(現)近畿産業信用組合 京都短期大学生活福祉:(現)花園福祉会龍雲寺保育園 夙川学院短期大学家政学科 京都短期大学生活福祉 OMMメディカルセンター Kyoto Junior College Kyoto Junior College Kyoto Junior College Kyoto Junior College Shukugawa Gakuin College Kyoto Junior College OMM Medical Center

出版者

京都短期大学成美学会

雑誌

京都短期大学紀要 = Bulletin of Kyoto Junior College (ISSN:13483064)

巻号頁・発行日

vol.34, no.1, pp.21-28, 2006-03-01

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When we made salt a water solution,a difference of taste of a component ratio of a main element depended on the sodium concentration,but the possibility that a mineral except sodium participated in "taste" was shown in a rice ball when we seasoned it.