著者
IKEHATA Kei HIRATA Takafumi
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical sciences (ISSN:09106340)
巻号頁・発行日
vol.29, no.12, pp.1213-1217, 2013-12
被引用文献数
19

We evaluated the capabilities of an in situ method for measuring copper isotopes of cubanite using UV-fs-LA-MC-ICP-MS. A comparison of the UV-fs laser results with those obtained from the NIR-fs laser system shows that there is obviously an improvement in the precision (<0.10‰, 2SE) when using the UV-fs laser. In both wavelength modes, matrix-matched standards are required for reliable in situ copper isotope analysis of cubanite. This method was applied to determinations for copper isotopes of minute cubanite grains in a skarn ore. Copper isotopic ratios of cubanite grains near a weathered surface of the sample are lower than those of intact cubanite grains within the sample, suggesting that selective leaching of heavier copper isotope in primary minerals occurred during weathering.
著者
Yan ZHANG Xiaoyan HUANG Wenfang LIU Zeneng CHENG Chuanpin CHEN Lihui YIN
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.29, no.10, pp.985-990, 2013-10-10 (Released:2013-10-10)
参考文献数
34
被引用文献数
3 24

Illegal chemicals, which could cause unpredictable side effects, may be added into traditional Chinese medicine (TCM) for a rapid healing effect. In this report, a surface-enhanced Raman scattering (SERS) analysis method for five kinds of illegally added drugs (rosiglitazone maleate, phenformin hydrochloride, metformin hydrochloride, pioglitazone hydrochloride and sibutramine hydrochloride) in Chinese traditional patent medicine (CTPM) has been demonstrated, including simultaneous detections of drug mixtures with CTPM. Silver colloidal, prepared by a sodium citrate reaction, was used as a SERS substrate. The optimum pH condition for each drug has also been explored because of its combined effect on protonation, surface charge, repulsion of an analyte and nanoparticles. Furthermore, the simultaneous detection of two or three kinds of these chemicals has been carried out. Characteristic peaks are employed for qualitative analysis. This is the first research using SERS for the analysis of drug mixtures in CTPM without any separation process.
著者
Wesley W. HSIAO Hsien-Shun LIAO Hsing-Hung LIN Yueh-Lun LEE Chia-Kwung FAN Chien-Wei LIAO Po-Yen LIN En-Te HWU Chia-Seng CHANG
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.29, no.9, pp.885-892, 2013-09-10 (Released:2013-09-10)
参考文献数
29
被引用文献数
4 12

Toxocarosis is a zoonosis caused by the transmission of the Toxocara canis (T. canis) larvae to humans. Its infectious third-stage larvae can invade the brains of paratenic hosts. The resultant brain damage can result in cerebral toxocarosis (CT). Astrocytes have important neurotrophic and neuroprotective functions in the brain. Substantial studies have shown that astrocyte apoptosis may contribute to the pathogenesis of many acute and chronic neurodegenerative disorders. We propose an alternation detection method, a combination of the astigmatic detection microscopy (ADM) and atomic force microscopy (AFM) techniques, to investigate the apoptosis of astrocytes triggered with T. canis larval excretory/secretory (Tc E/S) antigen. The variation in the pathology of a cell’s morphological changes was investigated with ADM and AFM analyses and then confirmed by western blotting. The results showed that the round cells increased as the concentration of Tc E/S antigen and incubated time increased. In addition, the mean height of apoptotic cells was approximately twice that of untreated normal cells, which meant there was correlation between the Tc E/S antigen treatment and cell height. For each cleaved caspase-3 in the cells cocultured with Tc E/S antigen and incubated for 9 h, the corresponding intensities increased about 34-fold (34.4 ± 1.8) compared with those of the control cells. This method can provide researchers with a perspective for understanding the limited information on the mechanism of astroglial injury and death during a T. canis larval invasion in a brain infection.
著者
Ildikó V. TÓTH Inês C. SANTOS Cláudia F. M. AZEVEDO Jorge F. S. FERNANDES Ricardo N. M. J. PÁSCOA Raquel B. R. MESQUITA António O. S. S. RANGEL
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.29, no.5, pp.563-570, 2013-05-10 (Released:2013-05-10)
参考文献数
30
被引用文献数
3 5

In this work, aiming to develop a simple, inexpensive method for the determination of low bromate levels in water samples, a liquid waveguide capillary cell (LWCC) was coupled to a FIA system. The long optical path (100 cm) of the LWCC was used to improve the sensitivity and the limit of detection without resorting to any off-line or in-line preconcentration processes. The spectrophotometric determination was based on the oxidation of chlorpromazine by bromate in an acidic medium, resulting in the formation of a colored radical product. Sulfamic acid was added to the reagent for minimizing the interference of nitrite, and a chelating ion exchange resin was used to remove major cationic interferences. The developed system allowed the determination of bromate within the range between 1 – 20 μg L−1 with a detection limit of 0.2 μg L−1.
著者
Tomoko FUKUUCHI Makoto YASUDA Katsunori INAZAWA Tatsuhiro OTA Noriko YAMAOKA Ken-ichi MAWATARI Kazuya NAKAGOMI Kiyoko KANEKO
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.29, no.5, pp.511-517, 2013-05-10 (Released:2013-05-10)
参考文献数
31
被引用文献数
6 25

Several methods for quantifying the purine content in food and drink have been described using high-performance liquid chromatography (HPLC). We have developed an improved HPLC method that is based on a method reported by Kaneko et al. and that is more sensitive yet simple, and suitable for determining the purine content of beer and beer-like alcoholic beverages. Quantitative HPLC separation was performed on a Shodex Asahi Pak GS-320HQ column with an isocratic elution of 150 mmol/L sodium phosphate buffer (H3PO4/NaH2PO4 = 20:100 (v/v)). The retention times for the four analytes, namely, adenine, guanine, hypoxanthine and xanthine, were 19.9, 25.0, 29.3 and 43.0 min, respectively. The resolution was good, and there was no excessive interference from the other compounds in the beverages at these retention times. Furthermore, the detection limit for all the analytes was improved to less than 0.0075 mg/L, and all the calibration curves showed good linearity (r2 > 0.999) between 0.013 and 10 mg/L for adenine and guanine, and between 0.025 and 10 mg/L for hypoxanthine and xanthine. The pretreatment was simplified by removing some procedures and optimizing the perchloric acid hydrolysis and the enzymatic peak-shift assay. We reduced the sample dilution rate by almost 50%, and the time spent on pretreatment from 4 days to only 180 min. The recovery of the analytes from spiked samples was 94.8 – 103.8%. This method may be useful for evaluating quantitative and qualitative differences in the purine content of beer and beer-like alcoholic beverages.
著者
Yoshio TAKAHASHI Kazuhiro KONDO Asami MIYAJI Miyuki UMEO Tetsuo HONMA Satoshi ASAOKA
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.28, no.10, pp.985-992, 2012-10-10 (Released:2012-10-10)
参考文献数
31
被引用文献数
9 15

Given that the supply of several rare earth elements (REEs) is sometimes limited, recycling REEs used in various advanced materials, such as Nd magnets, is important for realizing efficient use of REE resources. In the present work, the feasibility of using DNA for REE recovery and separation was examined, along with the identification of the binding site of REEs in DNA. In particular, a DNA-cellulose filter paper hybrid was prepared so that DNA-based materials can be used for the separation of REEs using columns loaded with DNA. N,N′-Disuccinimidyl was used as a cross-linker reagent for the fixation of DNA onto a fibrous cellulose filter. The results showed that (i) the DNA-filter hybrid has a sufficiently high affinity to adsorb REEs; (ii) the adsorption capacity was 0.182 mg/g for Nd; and (iii) the affinity of REEs for DNA was stronger for REEs with larger atomic numbers. The difference of the affinity among REEs in the third result was compared with the adsorption patterns of REEs discussed in the literature. The comparison suggests that phosphate in the DNA-filter paper hybrid was responsible for REE adsorption onto the hybrid. The results were supported by the Nd, Dy, and Lu LIII-edge EXAFS; the REE-P shell was identified for the second neighboring atom, showing the importance of the phosphate site as REE binding sites. The difference in the affinity among REEs suggest that group separation of REEs (such as La, Ce, (Pr and Nd), (Ho, Dy, and Er), (Tb and Gd), (Sm, Eu), Tm, Yb, and Lu) is possible, although complete isolation of each REE from a solution containing all REEs may be difficult. For practical applications, Nd and Fe(III) were successfully separated from a synthetic solution of Nd magnet waste using columns loaded with the DNA-filter hybrid.
著者
Hideo TAKEUCHI
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.27, no.11, pp.1077-1077, 2011-11-10 (Released:2011-11-10)
参考文献数
59
被引用文献数
14 55

UV Raman spectroscopy is a powerful tool for investigating the structures and interactions of the aromatic side chains of Phe, Tyr, Trp, and His in proteins. This is because Raman bands of aromatic ring vibrations are selectively enhanced with UV excitation, and intensities and wavenumbers of Raman bands sensitively reflect structures and interactions. Interpretation of protein Raman spectra is greatly assisted by using empirical correlations between spectra and structure. Many Raman bands of aromatic side chains have been proposed to be useful as markers of structures and interactions on the basis of empirical correlations. This article reviews the usefulness and limitations of the Raman markers for protonation/deprotonation, conformation, metal coordination, environmental polarity, hydrogen bonding, hydrophobic interaction, and cation-π interaction of the aromatic side chains. The utility of Raman markers is demonstrated through an application to the structural analysis of a membrane-bound proton channel protein.
著者
Elizabeth L. HOMMEL Gang MA Heather C. ALLE
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.17, no.11, pp.1325-1329, 2001 (Released:2005-04-20)
参考文献数
32
被引用文献数
22 45

An important advance in surface science has been the evolution of sum frequency generation to the application of studying surface structure and chemistry of liquid surfaces at the molecular-level by probing the vibrational signatures of surface molecules. Recently, broad-bandwidth sum frequency generation (BBSFG) spectroscopy has become an important tool for investigating gas-solid interfaces. BBSFG spectroscopy allows, theoretically, a surface sum frequency spectrum to be acquired within one pulse of the laser. In this paper, the viability of BBSFG to study inherently small nonlinear response interfaces and the time-resolving capability of this surface-selective technology are demonstrated. Presented here are the first published accounts of spectra from a liquid surface utilizing the broad-bandwidth sum frequency technology with acquisition times as low as 500 milliseconds.
著者
Mayumi MINAMISAWA Shoichiro YOSHIDA Nobuharu TAKAI
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.20, no.2, pp.325-328, 2004 (Released:2004-08-06)
参考文献数
22
被引用文献数
40 55

We studied the simultaneous quantitative analysis of biologically active substances, such as nicotinic acid, trigonelline, caffeine, qunolinic acid and tannic acid and pyrogallic acid, in several roasted coffees by an HPLC/diode-array system with a home-made sol-gel and ODS-2 columns. A simple method for simultaneous quantitative analysis of biologically active substances in the coffee brew became feasible by an HPLC/diode-array system with a sol-gel column at a single wavelength of 210 nm. The most efficient condition of the Rs value was above 1.05 when two sol-gel columns were connected. In addition, the elution behavior of nicotinic acid in brew extracted from commercially available coffee beans suggests the thermal decomposition process during roasting, and indicated the maximum value for full city roasted coffee.
著者
Hirohisa NAGATANI Satoshi DEJIMA Hiroki HOTTA Toru OZEKI Toshiyuki OSAKAI
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.20, no.11, pp.1575-1579, 2004 (Released:2005-03-03)
参考文献数
31
被引用文献数
5 8 2

The photocurrent at the polarized water/1,2-dichloroethane (DCE) interface was successfully observed in the presence of a lipophilic sensitizer, 5,10,15,20-tetraphenylporphyrinato zinc (ZnTPP), in the organic phase. The photocurrent transient responses were apparently affected by the employed organic supporting electrolyte: tetrapenthylammonium tetraphenylborate (TPnATPB) or tris(tetraoctylammonium)tungstophosphate ((TOcA)3PW12O40). The photocurrent measured in the TPnATPB system exhibited rather slow responses associated with the ion transfer of photoproducts. On the other hand, the photoinduced heterogeneous electron transfer could be observed in the use of (TOcA)3PW12O40. The photocurrent intensity in the (TOcA)3PW12O40 system exhibited an apparent pH dependence and the photoreduction of hydrogen ions probably took place at the water/DCE interface. By analyzing the real and imaginary components of the photocurrent depending on the photoexcitation frequency, we roughly estimated the phenomenological rate constants of the product separation (kps) and recombination (krec) processes as log(kps/s-1) = 1.5 ± 0.2 and log(krec/s-1) = 1.8 ± 0.1, respectively.
著者
Nikoleta URBANOVÁ Mihály KÁDÁR Klára TÓTH Botond BOGÁTI Vasil ANDRUCH István BITTER
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.24, no.6, pp.727-733, 2008 (Released:2008-06-10)
参考文献数
47
被引用文献数
5 12

Fluorescent sensor molecules were synthesized by conjugation of iminodiacetamide derivatives with fluorescent moieties of different structures and their UV-visible and fluorescent properties were characterized in acetonitrile solvent. The fluorescent measurements revealed that the N-(2-naphthyl) and N-phenyl derivatives exhibit a distinct zinc ion-selectivity over alkali and alkaline earth metal ions, while N-(anthrylmethyl) and N-(3-methoxyphenyl) derivatives do not possess any ion-selectivities. In contrast to the fluorescent measurements, all ligands show Zn2+ selectivity over Ca2+ and Mg2+ ions in plasticized PVC membranes using potentiometric signal transduction. This observation found for N-(anthrylmethyl) and N-(3-methoxyphenyl) derivatives can be ascribed to the more hindered interaction between the signalling group of the ionophore and the central metal ion in PVC membranes than in acetonitrile solution upon complexation. From the fluorescent measurements it can also be concluded that the ligands with metal ions form complexes mainly with 2:1 stoichiometry (L2M). On complex formation a considerable decrease in the fluorescent intensity was observed for all ligands except the N-(anthrylmethyl) derivative, where a 25 - 30 fold fluorescence enhancement was found, which is explained by the photoinduced electron transfer (PET) mechanism. All ionophores exhibited serious hydrogen ion interference, therefore complexation-induced spectral changes were measured in aprotic acetonitrile solution.
著者
Tatsuya SUZUKI Yuriko MUNAKATA Kazuki MORITA Tatsuya SHINODA Hiroshi UEDA
出版者
The Japan Society for Analytical Chemistry
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.23, no.1, pp.65-70, 2007 (Released:2007-01-10)
参考文献数
29
被引用文献数
23 41

Zearalenone (ZEA) is an estrogenic mycotoxin produced by Fusarium sp., and its production on corn and small grains during storage has been of considerable concern. For sensitive ZEA detection, we applied an open sandwich (OS) immunoassay that can noncompetitively detect monovalent antigens utilizing an antigen-induced enhancement of the VH/VL interaction. We cloned the VH and VL cDNAs of anti-ZEA mAb to a split-Fv phagemid pKST2, and firstly both VH and VL fragments were displayed on M13 phage p9 and p7, respectively, using an amber suppressor, TG-1, as a host. The split-Fv phage showed specific binding to immobilized ZEA, which was well inhibited by free ZEA. Then, the VH/VL interaction and its antigen-dependency were analyzed using a non-suppressor HB2151 as a host to produce VH-displaying phage and his/myc-tagged soluble VL in the culture supernatant. By capturing VL with an anti-myc or -his antibody and probing bound VH-phage, ZEA was successfully detected with a superior detection limit as well as a wider working range than those of a competitive assay. Also, essentially the same results were reproduced with purified VH-alkaline phosphatase and MBP-VL fusion proteins.