著者
石井 秀美 木崎 景一郎 平石 さゆり 堀江 修一 風間 睦美
出版者
日本リンパ網内系学会
雑誌
日本網内系学会会誌 (ISSN:03869725)
巻号頁・発行日
vol.31, no.4, pp.345-353, 1991 (Released:2009-06-04)
参考文献数
28

Influence of inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin 1-β (IL-1β) on the expression of thrombomodulin (TM, anticoagulant factor) and tissue factor-like procoagulant activity (PA, procoagulant factor) on cultured human umbilical vein endothelial cells and the effect of retinoic acid (RA, vitamin A acid) on the behavior of TM and PA in the cells caused by the cytokines were investigated. TNF-α and IL-1β induced the reduction of TM expression and elevation of PA in the cells in a dose-dependent manner in a range between 1 and 100U/ml. The reduction of TM expression by the cytokines accompanied with the decrease in TM mRNA levels, and the elevation of PA was inhibited by treatment with cycloheximide, inhibitor for protein synthesis. The current results indicate that the cytokines markedly increase procoagulant properties on the cell membrance through both the reduction of anticoagulant factor and the elevation of procoagulant factor, and suggest that the behaviors of anti- and pro-coagulant factor caused by the cytokines results from changes in protein synthesis. Treatment of the cells with RA prevented both the reduction of TM expression and the elevation of PA on the cell membrane caused by TNF-α treatment, in a dose-dependent manner in a range between 0.01 and 10μM. RA increased TM mRNA levels in the cells. It was suggested that RA regulates the balance between the expression of anti- and pro-coagulant factors on the cell membrance through regulation of gene transcription levels.
著者
尾形 隆 今井 大 市毛 明彦 山川 光徳 山田 和彦 大類 広 柘植 通 新野 恵司 鈴木 明彦 角田 卓哉 湯田 文朗
出版者
日本リンパ網内系学会
雑誌
日本網内系学会会誌 (ISSN:03869725)
巻号頁・発行日
vol.35, no.6, pp.319-331, 1995

The expression of adhesion molecules on human tonsillar follicular dendritic cells (FDCs) <i>in vivo</i> on cryostat sections and <i>in vitro</i> on isolated FDCs on cytospin preparations was studied. Isolation of FDCs was performed using a magnetic cell sorter (MACS). Immunochemically, FDCs were positive for Mac-1 (CD11b), sialyl-Le<sup>x</sup> (CD15s), CD22, integrin &beta;1 (CD29), CD40, VLA-&alpha;3 (CD49c), VLA-&alpha;5 (CD49e), VLA-&alpha;6 (CD49f), ICAM-3 (CD50), ICAM-1 (CD54), B7 (CD80), and VCAM-1 (CD106). These adhesion molecules, except ICAM-3 (CD50) which was weakly positive only in the light zone (LZ), were positive with a lacy pattern in all zones of secondary lymphoid follicle, especially strong in the LZ. ICAM-3 (CD50) was positive on more than half of isolated FDCs, but other molecules were positive on almost all isolated FDCs. Concerning the ligands on B cells for these adhesion molecules, the Mac-1 (CD11b)-ICAM-1 (CD54), the ICAM-3 (CD50)-LFA-1 (CD11a/18), and the VCAM-1 (CD106)-VLA-4 (CD29/49d) interactions in the LZ, the sialy-Le<sup>x</sup> (CD15s)-L-selectin (CD62L) and the VCAM-1 (CD106)-VLA-4 (CD29/49d) interactions in the mantle zone, and the ICAM-1 (CD54)-LFA-1 (CD11a/18) interaction in the entire lymphoid follicle may participate in FDC-B cell adhesion. Furthermore, above mentioned immunohistochemical evidence that FDCs were positive for fibronectin-receptor (CD29/CD49e) and laminin-receptor (CD29/CD49f), was confirmed with a frozen-section binding assay, using isolated FDCs and cryostat sections of tonsils. The numbers of FDCs binding to germinal centers (GCs) were reduced remarkably by pretreatment with monoclonal antibodies for CD29 (32.8&plusmn;4.1% of the control), CD49e (29.7&plusmn;2.5%), and CD49f (18.8&plusmn;0.8%). These data clearly indicated that FDCs bind to the reticulin or laminin fiber in GCs via either receptor.
著者
小池 健一 小宮山 淳
出版者
日本リンパ網内系学会
雑誌
日本網内系学会会誌 (ISSN:03869725)
巻号頁・発行日
vol.33, no.4-5, pp.229-234, 1993 (Released:2009-06-04)
参考文献数
8

乳児期より難治性口腔内カンジダ症を反復した姉弟の単球は走化能,カンジダ貪食殺菌能,IL-1産生能に異常を示したが,好中球機能やTおよびBリンパ球機能は正常であった。これらのことから真菌に対する感染防御の上で単球は重要な役割を果たしていると思われる。血球貪食症候群患児の血中サイトカイン濃度を測定し,臨床症状,検査所見の推移と比較した。2例とも増悪期においてIFN-γは著明な高値を示したが,TNF-αは全経過を通して正常範囲内であり,IL-1β, GM-CSFも正常もしくは軽度の上昇にとどまった。これらの結果はHPSでみられる組織球の活性化にIFN-γが最も重要な役割を演じていることを示している。JCMLのGM造血前駆細胞は種々のサイトカインの単独あるいは組み合わせに反応して正常骨髄よりも多数の,より大きなコロニーを形成し,増殖した細胞の大部分はマクロファージであった。また,JCMLのGM前駆細胞は正常対照と比較してtyrosine kinase inhibitorに抵抗性を示したことから,本症ではサイトカインによるparacrine増殖だけでなく造血幹細胞自体にも異常があることが示唆された。単球,マクロファージは貪食殺菌能に加えて,抗原提示能,サイトカイン分泌能を有し,リンパ球との間で巧妙な免疫機構を構築している。マクロファージを活性化するサイトカインにはinterleukin-1 (IL-1), tumor necrosis factor (TNF), interferon-γ (IFN-γ), granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor (M-CSF)などがある。単球,マクロファージの機能異常をきたす疾患の臨床像,病態についてはいまだ不明な点が多い。本稿では,単球,マクロファージの機能異常を機能低下と機能亢進に分け,サイトカインとの関連を中心にわれわれの成績を述べ,これらの病態について検討した。
著者
Hiroaki Tanaka Ikuo Sakuma Shinichiro Hashimoto Yusuke Takeda Shio Sakai Toshiyuki Takagi Takanori Shimura Chiaki Nakaseko
出版者
日本リンパ網内系学会
雑誌
Journal of Clinical and Experimental Hematopathology (ISSN:13464280)
巻号頁・発行日
vol.52, no.1, pp.67-69, 2012 (Released:2012-06-06)
参考文献数
13
被引用文献数
9 25

It has recently been reported that hepatitis B virus (HBV) reactivation in patients with hepatitis B surface antigen (HBsAg)-negative lymphoma during or after cytotoxic therapy occurs after the use of rituximab and stem cell transplantation for hematologic malignancies. However, clinical data on HBV reactivation in multiple myeloma patients have not been extensively reported. This is the first reported case of HBV reactivation in an HBsAg-negative myeloma patient treated with bortezomib (BOR) as salvage therapy and not stem cell transplantation. By closely monitoring HBV-DNA and early administration of entecavir, severe hepatitis was avoided and BOR therapy was continued. We suggest the importance of close monitoring of HBV-DNA for transplant-ineligible myeloma patients treated with BOR as salvage therapy. [J Clin Exp Hematopathol 52(1) : 67-69, 2012]