著者

MINORU SANADA TETSUJI MIYANO SHUICHI IWADARE

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.39, no.2, pp.304-305, 1986 (Released:2006-04-19)

参考文献数

3

被引用文献数

6 11

著者

Teiichirō Itō Motohiro Nishio Hiroshi Ogawa

出版者

Japan Antibiotics Research Association

雑誌

The Journal of Antibiotics, Series A (ISSN:03681173)

巻号頁・発行日

vol.17, no.5, pp.189-193, 1964 (Released:2020-07-07)

参考文献数

14

---

In the course of a research for kanamycin (kanamycin A)1) purification, kanamycin B1) and kanamycin C2) were isolated from the culture broth of Streptomyces kanamyceticus1). Kanamycin B was obtained in pure form by Schmitz, Fardig, O’Herron, Rousche and Hooper8), who isolated deoxystreptamine and 3-amino-3-deoxy-D-glucose after acid hydrolysis of N-acetyl-kanamycin B. Wakazawa, Sugano, Abe, Fukatsu and Kawaji4) reported that partial hydrolysis mixture of kanamycin B still retained some biological activity against B. subtilis, and later they5) isolated a crystalline degradation product which had antibacterial properties.It has been determined that in kanamycins A6,7,8,9) and C10) two aminosugars link to 4- and 6-positions of deoxystreptamine, while in neomycin-group (neomycins B C11,12d) and paromomycin13) the 4,5 hydroxyls of deoxystreptamine are substituted. It should be noted that neomycins are more toxic than kanamycins A and C. As compared with kanamycin A and neomycin S, kanamycin B has more than twice less toxicity than neomycins and has more than five times toxicity than kanamycin A. As reported by Wakazawa et. al.4), kanamycin B can be determined separately from A.In the present paper, our experiments on the structure of kanamycin B are described. Kanamycin B base, which was prepared by the process of Wakazawa et al4) and was recrystallized from ethanol-water, showed [α]24D+ 126° (c, 0.7 in water) and decomposed over the range of 170~190°C. It was soluble in water, but hardly soluble in organic solvent. Its molecular weight was reported to be 1,170 (Rast method, urea as solvent) by Schmitz et al.3) and 385~560 (Barger method, water as solvent) by Wakazawa et al.4) The crystals obtained in our laboratory showed molecular weight of 430~590 by the Rast method (urea as solvent, kanamycin A as standard).N-Acetylkanamycin B3,4) was prepared and, from its hydrolyzates, deoxystreptamine hydrochloride (molecular weight 235) was isolated in the yield of about 30% by weight. The elemental analyses of kanamycin B base and its N-acetyl derivative, together with the above data, suggested the molecular formula C18H37N5O10 for kanamycin B base.

著者

A. W. JOHNSON ROGER M. SMITH

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.25, no.5, pp.292-297, 1972-05-25 (Released:2006-04-12)

参考文献数

22

被引用文献数

3 2

---

Further hydrolytic and oxidation studies on the antibiotic vancomycin have resulted in a revision of the structure of one of the aromatic units and the identification of N-methylleucine as a terminal group.

著者

M.A.Q. SIDDIQUI D. NICHOLSON PHILIP J. SNIDER

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.25, no.1, pp.54-59, 1972-01-25 (Released:2006-04-12)

参考文献数

16

被引用文献数

1

---

The action of streptomycin on protein synthesis was examined in vivo and in vitro with Serratia marcescens. At lethal concentrations of streptomycin (10μg/ml and above) inhibition of protein synthesis and cell death appear to be causally related. Lower concentrations of the antibiotic (4-5μg/ml) do not reduce protein synthesis, growth or the viability of the sensitive cells even after prolonged incubation in the presence of the drug. However, ribosomes isolated from cells so treated show partial reduction of ammo acid incorporation in vitro in the absence of added streptomycin. Evidence suggests that subtle structural defects induced during ribosomal synthesis in the presence of the antibiotic and binding of streptomycin to the ribosomes together account for the defective ribosomal functioning during protein synthesis in vitro.

著者

JUN'ICHI SHOJI HIROSHI HINOO KOICHI MATSUMOTO TERUO HATTORI TADASHI YOSHIDA SHINZO MATSUURA EIJI KONDO

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.41, no.6, pp.713-718, 1988-06-25 (Released:2006-04-19)

参考文献数

5

被引用文献数

32 45

---

Two peptide antibiotics katanosins A and B were isolated from the culture broth of a strain related to the genus Cytophaga. These antibiotics are basic peptides soluble in aqueous alcohols. The molecular formulae C57H95N15O17 for A and C58H97N15O17 for B were indicated. The constituent amino acids of katanosin A are suggested to be Thr (1), Ser (1), Val (1), Leu (3), Arg (1) and three unusual amino acids. In katanosin B, the Val residue is replaced by He. Katanosins A and B are active against Gram-positive bacteria in vitro and in vivo.

著者

WEIMIN HU SHIGEKI OHYAMA KEIJI HASUMI

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.53, no.3, pp.241-247, 2000-03-25 (Released:2008-09-19)

参考文献数

21

被引用文献数

41 46

---

Two novel staplabin analogs, SMTP-7 and -8, have been isolated from cultures of Stachybotrys microspora IFO 30018. Spectroscopic analyses showed that the SMTP-7 molecule consisted of two identical staplabin core structures and ornithine which bridges the two partial structures. In the SMTP-8 molecule, the bridging unit was lysine. At concentrations of 80-150μM, the two compounds caused 2- to 12-fold increase in urokinase-catalyzed plasminogen activation, fibrin binding of plasminogen, and urokinase- and plasminogen-mediated fibrinolysis. These activities of SMTP-7 and -8 were two to ten times higher than those of staplabin and previously isolated SMTPs, which exerted such effects at concentrations ranging from 150 to 800 μM.

著者

U. TAUBENECK

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.31, no.11, pp.1209, 1978 (Released:2006-04-12)

著者

SHANG GUANGDONG DAI JIANLU WANG YIGUANG

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.54, no.1, pp.66-73, 2001-01-25 (Released:2008-09-19)

参考文献数

18

被引用文献数

14 23

---

Shengjimycin is a group of 4′′-acylated spiramycins with 4′′-isovalerylspiramycin as the major component, produced by recombinant S. spiramyceticus F21 harboring a 4′′-O-acyltransferase gene from S. mycarofaciens 1748. A stable bioengineered strain of Streptomyces spiramyceticus WSJ-1 was constructed by integrating the 4′′-O-acyltransferase gene (ist) by homologous recombination into the chromosome of the spiramycin-producing strain S. spiramyceticus F21. In this construction, a Streptomyces/E. coli shuttle plasmid pKCl39 (AmR) was used as the vector with the tsr gene used as selection marker for homologous recombination. The constructed strain, S. spiramyceticus WSJ-1, was genetically stable in production titer and proportion of components of Shengjimycin as well as in maintaining the tsr selective marker when grown without selection. Southern hybridization confirmed the integrated status of the ist gene in the host genome. The production and the proportion of major component of 4′′-isovalerylspiramycin of S. spiramyceticus WSJ-1 was also improved comparing with the strain harboring an autonomous plasmid -S. spiramyceticus F21/pIJ680(311) as shown by HPLC analysis. Physiological studies indicated that increase of the VDH (valine dehydrogenase) and LDH (leucine dehydrogenase) activities of WSJ-1 may be involved in this improvement.

著者

YOSHINORI YAMASHITA YUTAKA SAITOH KATSUHIKO ANDO KEIICHI TAKAHASHI HIROE OHNO HIROFUMI NAKANO

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.43, no.10, pp.1344-1346, 1990-10-25 (Released:2006-04-19)

参考文献数

6

被引用文献数

14 33

著者

TOSHIO OTANI YOSHINORI MINAMI TERUYOSHI MARUNAKA RUI ZHANG MEI-YU XIE

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.41, no.11, pp.1696-1698, 1988-11-25 (Released:2006-04-19)

参考文献数

11

被引用文献数

9 5

著者

TOSHIO OTANI YOSHINORI MINAMI TERUYOSHI MARUNAKA Rui ZHANG MEI-YU XIE

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.41, no.11, pp.1580-1585, 1988-11-25 (Released:2006-04-19)

参考文献数

15

被引用文献数

57 67

---

A new macromolecular antibiotic C-1027 was obtained from the broth filtrate of Streptomyces ghbisporus C-1027 by precipitation with ammonium sulfate, DEAE-cellulose column chromatography and gel filtration chromatography on a Sephadex G-75 column. This antibiotic, prepared as a white powder, is an acidic polypeptide having an isoelectric point of pH 3.5-3.7 and a molecular weight of 15, 000 as determined by SDS-polyacrylamide gel electrophoresis and gel filtration chromatography. The acid hydrolysate of the purified antibiotic C-1027 contained no methionine or tryptophan. From the physico-chemical data, it may be considered to possess a very labile non-protein chromophore.

著者

JILAN HU YU-CHUAN XUE MEI-YU XIE RUI ZHANG TOSHIO OTANI YOSHINORI MINAMI YUJI YAMADA TERUYOSHI MARUNAKA

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.41, no.11, pp.1575-1579, 1988-11-25 (Released:2006-04-19)

参考文献数

14

被引用文献数

77 102

---

Strain C-1027, an actinomycete isolated from a soil sample collected in China, was found to produce the new antibiotic, C-1027. From taxonomical studies on its morphological, cultural and physiological characteristics, this antibiotic-producing strain was identified as Streptomyces ghbisporus C-1027. Antibiotic C-1027 has antimicrobial activity against most Gram-positive bacteria but not against Mycobacterium sp. or Gram-negative bacteria. This antibiotic shows remarkable activity in spermatogonial assay and potent cytotoxicity against KB carcinoma cells in vitro, and exhibits inhibition on transplantable tumors in mice.

著者

KEISUKE ISHIDA TAKAYUKI TERUYA SIRO SIMIZU HIROYUKI OSADA

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.57, no.2, pp.136-142, 2004-02-25 (Released:2009-01-27)

参考文献数

32

被引用文献数

5 12

---

In this paper we describe the establishment of an efficient visual method for screening heparanase inhibitors, and we present the results of screening 10, 000 microbial culture broths. Heparanase-overexpressing stable clones of the human hepatocellular carcinoma HepG2 cells were established and used as an enzyme source. Digestion of heparan sulfate (HS) was detected using novel HS-containing tablets or SDS-polyacrylamide gel electrophoresis. This method was able to find suramin, a known heparanase inhibitor, from a library of typical enzyme inhibitors. By screening 10, 000 culture broths of microorganisms (actinomycetes, fungi, and bacteria) an actinomycete strain, RK99-A234, was found to have heparanase inhibitory activity. RK-682 was identified in the fermentation broth as a heparanase inhibitor, IC50=17μM.

著者

TOM S. CHEN BYRON H. ARISON LINDA S. WICKER EDWARD S. INAMINE RICHARD L. MONAGHAN

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.45, no.1, pp.118-123, 1992-01-25 (Released:2006-04-19)

参考文献数

7

被引用文献数

15 17

---

The immimosuppressants FK506 and FR 900520 were desmethylated by Actinoplanes sp. ATCC 53771 to yield various O-desmethylated products. The products were isolated and purified by solvent extraction and HPLC chromatography, and identified by NMR and MS spectroscopy.

著者

YUSUKE TOKORO TOSHIYUKI ISOE KAZUTOSHI SHINDO

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.52, no.3, pp.263-268, 1999-03-25 (Released:2008-09-19)

参考文献数

5

被引用文献数

7 9

---

A new antitumor antibiotic gilvusmycin was isolated from the culture broth of Streptomyces sp. QM16. The structure of gilvusmycin was related to CC-1065 and determined by NMR spectral analysis. Gilvusmycin exhibited antitumor activity against murine leukemia P388 in vivo.

著者

VINCENT L. REYNOLDS J. PATRICK MCGOVREN LAURENCE H. HURLEY

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.39, no.3, pp.319-334, 1986 (Released:2006-04-19)

参考文献数

42

被引用文献数

76 87

著者

DAVID G. MARTIN CAROLYN BILES SHIRLEY A. GERPHEIDE LADISLAV J. HANKA WILLIAM C. KRUEGER J. PATRICK MCGOVREN STEPHEN A. MIZSAK GARY L. NEIL JULIANNA C. STEWART JERONIMO VISSER

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.34, no.9, pp.1119-1125, 1981 (Released:2006-04-12)

参考文献数

8

被引用文献数

80 104

---

( Received for publication June 15, 1981)CC-1065 (NSC 298223) is a potent new antitumor antibiotic with a unique structure produced by Streptomyces zelensis. Improved production, isolation, and assay methods are described along with physico-chemical properties and antitumor activity.

著者

L. J. HANKA A. DIETZ S. A. GERPHEIDE S. L. KUENTZEL D. G. MARTIN

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.31, no.12, pp.1211-1217, 1978 (Released:2006-04-12)

参考文献数

23

被引用文献数

141 205

---

A new antitumor antibiotic is produced in fermentation liquors of Streptomyces zelensis sp.n. The antibiotic is biologically active at extremely low concentrations. At 40 pg/ml, it inhibited 90% of the growth of L1210 cells in culture in tube dilution assays. The minimal inhibitory concentrations against Gram-positive bacteria is between 1-10 ng/ml, while these values for Gram-negative bacteria and fungi are mostly under 1 μg/ml. A microbiological assay with Bacillus subtilis can detect concentrations of 1-2 ng/ml.

著者

TOSHIAKI HAYASHI TAKAO NOTO YOSHIHARU NAWATA HIROSHI OKAZAKI MIKIO SAWADA KUNIO ANDO

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.35, no.7, pp.771-777, 1982 (Released:2006-04-12)

参考文献数

17

被引用文献数

16 25

---

A new antibiotic, cyanocycline A, was isolated from the fermentation broth of Streptomyces flavogriseus strain No. 49, a soil isolate. The molecular formula of cyanocycline A was determined to be C22H26N4O5. The antibiotic has a cyano group and a N-heterocyclic quinone moiety in its structure. Cyanocycline A was found to have broad spectrum antimicrobial and antitumor activity.

著者

HIROSHI NISHIOKA TSUTOMU SAWA KUNIO ISSHIKI YOSHIKAZU TAKAHASHI HIROSHI NAGANAWA NAOKO MATSUDA SEIKO HATTORI MASA HAMADA TOMIO TAKEUCHI KAZUO UMEZAWA

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.44, no.11, pp.1283-1285, 1991-11-25 (Released:2006-04-19)

参考文献数

8

被引用文献数

6 12