- 著者
-
岸保 鉄也
霜村 真一
久松 健一
中澤 勉
中島 司
野沢 出
村上 嘉彦
- 出版者
- 日本耳科学会
- 雑誌
- Otology Japan (ISSN:09172025)
- 巻号頁・発行日
- vol.4, no.1, pp.90-94, 1994
In middle ear diseases including otitis media with effusion (OME), there are various lipids mediators such as leukotoriene C<SUB>4</SUB>, D<SUB>4</SUB> (LTC<SUB>4</SUB>, LTD<SUB>4</SUB>) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) in the effusion of the tympanic cavity. Therefore, it is important to examine the effect of these mediators on middle ear clearance. We studied on the effect of LTC<SUB>4</SUB>, LTD<SUB>4</SUB> and PGE<SUB>2</SUB> on mucociliary transport of the eustachian tube both in vitro and in vivo. Healthy guinea pigs with normal Preyer's reflexes were used in vitro study. Normal ciliated epithelium was carefully obtained from the tubotympanium and incubated with RPMI solution in the from of tissue culture. The epithelial specimens were incubated with LTC<SUB>4</SUB>, LTD<SUB>4</SUB> and PGE<SUB>2</SUB> respectively, ranged at the concentrations of 10<SUP>-8</SUP>M and 10<SUP>-6</SUP>M. Ciliated cells of the specimens were observed under an inverted microscope. Ciliary activity of each ciliated cell was photo-electrically measured on a TV monitor. LTC<SUB>4</SUB> and LTD<SUB>4</SUB> inhibited ciliary activity at the concentrations of 10<SUP>-8</SUP>M and 10<SUP>-6</SUP>M in vitro. PGE<SUB>2</SUB> promoted ciliary activity at the concentrations of 10<SUP>-8</SUP>M and 10<SUP>-6</SUP>M. Healthy chinchillas were used in vivo study. The animals were free of middle ear infection and hearing loss as determined by otomicroscopy, tympanometry and auditory brainstem response (ABR). One ml each of 10<SUP>-5</SUP>M LTC<SUB>4</SUB>, LTD<SUB>4</SUB>, PGE<SUB>2</SUB> and the control solution (10<SUP>-3</SUP> ethanol/saline) was directly injected into the tympanic bulla with a 27-gauge syringe under anesthetization. The middle ears were examined by otomicroscopy, tympanometry and ABR across time. LTC<SUB>4</SUB> and LTD<SUB>4</SUB> inhibited mucociliary transport of the eustachian tube. However, there was no significant difference between PGE<SUB>2</SUB> and the control.