著者

奥田 峰広 吉池 高志

出版者

公益社団法人 日本皮膚科学会

雑誌

日本皮膚科学会雑誌 (ISSN:0021499X)

巻号頁・発行日

vol.110, no.13, pp.2115, 2000 (Released:2014-08-19)

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皮膚の健常性を維持するためには,皮膚を清潔に保つことが重要であり,そのために各種身体用洗浄剤が古くより用いられてきた.一方,近年アトピー性皮膚炎などの皮膚疾患と角層バリア機能との関連性が注目されている.そこで,本報では皮膚洗浄,特に洗浄剤中の界面活性剤やpHそして洗浄操作と角層バリア機能との関連について比較検討を行った.その結果,洗浄操作を行うことで角層水分蒸散量やリボフラピン浸透量を指標とした角層のバリア機能が影響を受けるだけでなく,その程度,すなわちこする操作の程度で角層バリア機能への影響が異なることも確認された.また,緩衝液を用いた洗浄では角層への影響は少ないが,界面活性剤を含むことで角層への影響に差が認められ,アルカリ性(pH9)では角層への影響が大きくなり,穏和な洗浄条件であっても角層細胞間脂質の溶出などの影響が認められた.一方,弱酸性(pH5)では角層バリア機能への影響も少ないことが明らかとなり,皮膚を洗浄する条件としては皮膚の生理的pHに近い弱酸性が望ましいと考えられた.

著者

吉村 政哲 城倉 洋二 花沢 英行 野崎 利雄 奥田 峰広 芋川 玄爾

出版者

The Society of Cosmetic Chemists of Japan

雑誌

日本化粧品技術者会誌 (ISSN:03875253)

巻号頁・発行日

vol.27, no.3, pp.249-254, 1993-12-16 (Released:2010-08-06)

参考文献数

20

被引用文献数

4 1

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Lauroyl beta-alanine (LBA) is a unique amino acid derivative-surfactant which has recently been found to have very low potential of inducing a specific inflammationrelated receptor on epidemal cells, intercellular adhesion molecule-1 (ICAM-1) when incubated with human keratinocytes. This led us to assume that this surfactant possesses very low cutaneous inflammatory properties as opposed to ordinary commercially available anionic surfactants. Thus, we have assessed the biologic effects of LBA on several cellular derangements in the stratum comeum, cytotoxicity against human keratinocytes and an influence on arachidonic metabolism in skin tissue in comparison with those by other ordinary anionic surfactants such as potassium myristate (SOAP), sodium dodecyl sulfate (SDS), sodium cocoyl isethionate (SCI), acylmethyl taurine (AMT) and monoalkyl phosphate (MAP).Permeability experiments using hairless pig skin indicated that LBA is 10 and 3 times as lower permeable as SOAP and MAP, respectively. In in vitro study on human keratinocytes, of all anionic surfactants used, LBA showed the lowest inhibitory effect on cell growth which was accompanied by a lower level of the release of arachidonic metabolite such as prostaglandin E2 (PGE2) from human keratinocytes. This mild cellular effect was also corroborated by the previous observations that LBA elicits on substantial expression of ICAM-1 which has recently been identified on surface of epidermal cells in inflammatory dermatosis typified by T cell infiltration, in contrast to a marked expression by some of other anionic surfactants.In order to clarify in vivo cutaneous effect, cumulative cup shaking test was carried out on the inner surface of human forearm skin by applying surfactant aqueous solutions twice a day for four days. Whereas almost all anionic surfactants induced severe scaling and erythematous reactions during repeated treatments, LBA was the only surfactant which did not elicit any roughness and inflammatory reaction. This non-inflammatory property was also corroborated by an additional study on damaged skin that roughened forearm skin after acetone/ether treatment can be slightly restored even by successive applications of LBA, but not other anionic surfactants used. These findings indicate that LBA has biologically low active properties to both stratum coreum and epidermal cells which may lead to the development of a unique surfactant applicable to damaged skins.

著者

沼野 香世子 石崎 千明 佐藤 紀子 秋葉 俊一 堀田 光行 北原 隆 佐野 裕 奥田 峰広

雑誌

日本小児皮膚科学会雑誌 = Journal of pediatric dermatology (ISSN:02869608)

巻号頁・発行日

vol.28, no.2, pp.219-226, 2009-11-30