7 0 0 0 OA どうするタンパク質結晶構造解析 ―生体高分子構造研究におけるX線結晶学―
- 著者
- 山本 雅貴
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.65, no.1, pp.55-63, 2023-02-28 (Released:2023-03-08)
- 参考文献数
- 39
In modern life science research, structural information on proteins is indispensable for understanding the mechanisms of their biological functions. Many protein structures have been determined by X-ray crystallography, which has been developed in conjunction with molecular biology experiments and synchrotron radiation development. Since the Nobel Prize in Chemistry in 2017, the rapid progress of cryo-EM hardware and analysis software has led to the use of cryo-EM single-particle analysis for structural analysis. In this article, we compare the features of protein crystallography and cryo-EM single-particle analysis and discuss the best method for protein crystallography.
2 0 0 0 OA シンクロトロン放射光による複数結晶を用いたタンパク質構造解析技術
- 著者
- 平田 邦生 山本 雅貴
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.59, no.4, pp.215-218, 2019 (Released:2019-07-25)
- 参考文献数
- 12
1 0 0 0 OA SPring-8のタンパク質結晶構造解析
- 著者
- 山本 雅貴 平田 邦生 長谷川 和也
- 出版者
- 日本結晶学会
- 雑誌
- 日本結晶学会誌 (ISSN:03694585)
- 巻号頁・発行日
- vol.64, no.1, pp.2-9, 2022-02-28 (Released:2022-03-04)
- 参考文献数
- 37
Since the first crystal structure determination of protein by J. C. Kendrew in 1959, protein crystallography has been playing the leading role in the structural biology by various technology developments. Especially the utilization of synchrotron radiation from the 1990s brought innovative progress in this field on the data quality and the phasing method and had expanded the sample targets including membrane proteins and supra-molecular complexes. Here we will describe the outline and the future prospects of the protein crystallography from the role of synchrotron radiation and SPring-8.