著者

後藤 友佳子

出版者

一般社団法人 日本耳鼻咽喉科学会

雑誌

日本耳鼻咽喉科学会会報 (ISSN:00306622)

巻号頁・発行日

vol.93, no.8, pp.1192-1201, 1990

被引用文献数

5

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The localization of epidermal growth factor (EGF) in human cholesteatoma tissue, normal ear drum and external auditory canal skin was examined immunohistochemically, using avidin-biotin peroxidase complex method.<br>Bouin-fixed tissue was stained for investigation of horny layer in the epidermis, because fixation in Bouin's solution provides better preservation of the antigen. In the horny layer of cholesteatoma tissue, 19 out of 24 cases had EGF-positive immunoreactivity (79%). In 2 cases of normal external auditory canal skin, 4 cases of normal ear drum and a case of postauricular skin, no EGF-immunoreactivity was revealed in the horny layer.<br>EGF was assayed in the debris of cholesteatoma and the horny layer of the normal bony external canal with dot blot immunoassay. EGF content of the debris was higher than that of the horny layer of normal skin.<br>The result of the first report suggests the activity of cholesteatoma exists in the subcutaneous tissue (see the previous paper). In this report EGF content of cholesteatoma in the horny layer was found higher than that of normal external skin. This result demonstrates that EGF in the horny layer plays an important role in accelerating the growth and bony destruction in cholesteatoma.<br>To summarize these two reports, the following conclusion was reached. In the epidermis EGF content is equal in cholesteatoma and normal skin. But in the subcutaneous tissue and the horny layer EGF content of cholesteatoma is higher than that of normal skin. EGF in situ may be strongly related to the growth and bony destruction of cholesteatoma.

著者

後藤 友佳子

出版者

The Oto-Rhino-Laryngological Society of Japan, Inc.

雑誌

日本耳鼻咽喉科学会会報 (ISSN:00306622)

巻号頁・発行日

vol.93, no.8, pp.1192-1201, 1990-08-20 (Released:2008-03-19)

参考文献数

41

被引用文献数

6 5

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The localization of epidermal growth factor (EGF) in human cholesteatoma tissue, normal ear drum and external auditory canal skin was examined immunohistochemically, using avidin-biotin peroxidase complex method.Bouin-fixed tissue was stained for investigation of horny layer in the epidermis, because fixation in Bouin's solution provides better preservation of the antigen. In the horny layer of cholesteatoma tissue, 19 out of 24 cases had EGF-positive immunoreactivity (79%). In 2 cases of normal external auditory canal skin, 4 cases of normal ear drum and a case of postauricular skin, no EGF-immunoreactivity was revealed in the horny layer.EGF was assayed in the debris of cholesteatoma and the horny layer of the normal bony external canal with dot blot immunoassay. EGF content of the debris was higher than that of the horny layer of normal skin.The result of the first report suggests the activity of cholesteatoma exists in the subcutaneous tissue (see the previous paper). In this report EGF content of cholesteatoma in the horny layer was found higher than that of normal external skin. This result demonstrates that EGF in the horny layer plays an important role in accelerating the growth and bony destruction in cholesteatoma.To summarize these two reports, the following conclusion was reached. In the epidermis EGF content is equal in cholesteatoma and normal skin. But in the subcutaneous tissue and the horny layer EGF content of cholesteatoma is higher than that of normal skin. EGF in situ may be strongly related to the growth and bony destruction of cholesteatoma.

著者

香山 智佳子 後藤 友佳子 長谷川 信吾 藤田 岳 丹生 健一

出版者

Japan Otological Society

雑誌

Otology Japan (ISSN:09172025)

巻号頁・発行日

vol.18, no.1, pp.39-44, 2008-03-21 (Released:2011-06-17)

参考文献数

20

被引用文献数

1

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Gentian violet (Pyoktanin) is known to have a potent antibacterial activity against Gram-positive bacteria including methicillin-resistantStaphylococcus aureus (MRSA).From November 2000 to August 2006, we have treated 91 patients for intractable ear infected with MRSA by topical treatment with gentian violet. There were 99 infected ears: 44 chronic otitis media, 11 otitis media with effusion treated with tympanostomy tube, 10 repetitive suppurative otitis media, 10 otitis externa, 8 postoperative discharging mastoid cavity for cholesteatoma, 6 cholesteatoma, 4 eosinophilic otitis media, 3 acute otitis media, 3 myringitis. The pathogens detected in those ears during the treatment period were as follows: MRSA in 92 ears, MRSA andP. aeruginosa7 ears. Topical treatment with gentian violet was performed 7.5 times on average. In the 92 ears (92.9%), topical treatment was performed 5.4 times on average. Noteworthily, 23 ears required only one treatment and 16 ears required twice, and this fact was suggesting that frequent treatments were not necessary to control the pathogens sensitive to gentian violet. This high cure rate (92/99) of the ear infected with MRSA also suggested that this treatment was especially effective for the treatment of MRSA infected ears.