著者
斎藤 隆三 横山 定助
出版者
昭和大学学士会
雑誌
昭和医学会雑誌 (ISSN:00374342)
巻号頁・発行日
vol.17, no.2, pp.141-146, 1957-05-30 (Released:2010-09-09)
参考文献数
11

In the present work, a few observations were made on the fixation of the tissues, particularly on the time between the collection and fixation of the tissues and its procedures, and the preparation of extra-thin sections with ultramicrotome, which are most essential for obtaining superior histological pictures in the electron microscopy.In the cases of the tissues obtained in the animal experiments, several drops of OsO4 solution were dripped on the tissue when the material was collected and the tissue shall be cut into oblong slices; slices shall be placed in OsO4 solution and cut into fine blocks of approximately 1 mm3 20-30 minutes later. The fine blocks of approximately 1 mm3 shall be obtained by 2-3 cuts with razor blade. On some occasions, autolytic changes are observed in the picture of the material prepared from the centre of the fine blocks fixed for 1-2 minutes. This is considered due to the time required for the OsO4 solution to penetrate the tissue. When mechanical force was added to this, the picture thus obtained might be misinterpreted. In the case of human tissue, surgical specimen is preferable, but, if too long time was spent for the operation, fine structures of the cells was lost, and conglomeration and sissolution of the granules contained in the nucleus were observed. These phenomena were particularly eminent in the cases of tumor cells. In the cases of autopsy material, unless the material was collected within 1 hour at the latest from the death, the significance of electron microscopy will be lost.
著者
横山 定治 垂水 彰二 小関 佐貴代 菊地 恵美 山田 幸子 早川 潔 八田 一
出版者
Japanese Society for Food Science and Technology
雑誌
日本食品科学工学会誌 : Nippon shokuhin kagaku kogaku kaishi = Journal of the Japanese Society for Food Science and Technology (ISSN:1341027X)
巻号頁・発行日
vol.49, no.3, pp.174-181, 2002-03-15
被引用文献数
1 3

鰹節のアルコール水抽出物を食塩10%,エタノール5%条件下,醤油麹で分解することで調味料を生産し,官能検査により調味効果を検討すると共に,抗高血圧作用に関与すると言われているアンジオテンシンI変換酵素阻害(ACEI)活性,およびγ-アミノ酪酸(GABA)含量を測定した. <BR>(1) 30℃, 3ケ月の分解熟成後の値はTN 1.53%, FN0.73%, pH 5.32であった.分解途中での酵母,乳酸菌を含め微生物の増殖は見られず,醤油臭が少なく,鰹節風味の豊富な調味料が得られた. <BR>(2) 得られた調味料の食塩濃度は11.8%で,濃口醤油より低い.平均ペプチド鎖長4.7は,醤油の3.5に比較して長鎖であった.HPLC-GPCの結果から,本調味料は醤油と比較して分子量1000-3000程度のペプチドを多く含むことが認められた. <BR>(3) 得られた調味液のACEI活性は10倍希釈液で77.6%であり,IC50は1.52mg protein/mlであった.また本調味料はGABAを35.1mg/100ml含有しており,これは135.0mg/100g solidに相当した.これらの結果から,この調味料が抗高血圧作用等の生体調節機能を示す可能性が考えられた. <BR>(4) 調味料としての有用性を確認する為に,汁物,煮物,酢の物などの調理における調味効果を2点評価法により醤油との比較に於いて官能評価した.塩分を同程度に調製したにも関わらず,清汁,天つゆ,煮物の調理において本調味料は醤油の場合よりも塩辛さが有意(p<0.1)に低く感じられ,酢のものでは酸味が有意に(p<5)抑えられまろやかな味に仕上がった.