著者
三谷 孝子 橋本 浩司 千葉 眞彰 池淵 研二 関口 定美
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.42, no.6, pp.294-298, 1996-12-01 (Released:2010-03-12)
参考文献数
17
被引用文献数
2 1

Bacterial contamination was observed in a platelet concentrate obtained by apheresis using COBE SPECTRA and stored for 45 hours with agitation at 20 to 24°C. The platelet product was contaminated with 107 CFU/ml of Bacillus cereus. The contamination was likely attributable to bacteria on the skin at the phlebotomy site, because 1) apheresis was performed in a closed system and 2) Bacillus cereus are known to exist ubiquitously including the skin surface, and to be resistant to the chlorohexidine-ethanol that is routinely used for disinfection of phlebotomy site in our blood center. In a spike experiment with apheresis platelets, the growth of Bacillus cereus was vey rapid, with a doubling time of 6 hours to reach 107 CFU/ml in 2 days.Leukocyte depletion resulted in reduced phagocytic activity and allowed rapid growth of the contaminating bacteria in platelet products. There findings enpharize the importance of preventing as far as possible. In addition, platelet recipients, largely comprising leukemia patients, are vulnerable to infection.
著者
山本 律子 倉田 寛一 池淵 研二 三輪 哲義 村井 善郎 森 眞由美
出版者
一般社団法人 日本血液学会
雑誌
臨床血液 (ISSN:04851439)
巻号頁・発行日
vol.27, no.3, pp.321-326, 1986 (Released:2009-02-02)
参考文献数
16

From January 1979 to March 1985, febrile episodes occurring in 35 aged patients (maen age 76 years old) with leukemia were reviewed. There were 2.2 febrile episodes per patient on an average. Eighty-two percent of the febrile episodes was due to documented infections. The most common types of infections were septicemia and pneumonia, which together accounted for 61% of the total infections.Causative microorganisms were identified in 59% of the total febrile episodes. Gram-negative bacilli were responsible for only 46% of the microbiologically documented febrile episodes, while 40% of episodes was caused by gram-positive cocci. The use of new broad-spectrum antibiotics seems to have increased the frequency of gram-positive-cocci infections. Twenty-nine percent of the patients had repeated episodes of infections caused by the same organisms.As has been generally pointed out about the infection in the aged, infections caused by multiple organisms were very frequent. The most common combination, gram-positive cocci and gram-negative bacilli, accounted for 64% of these infections.Sixty-seven percent of febrile episodes occurred when the patients had neutropenia (less than 500/mm3), while 29% of the episodes occurred when the patients had enough neutrophils (more than 1,000/mm3). The fatality rate during febrile episodes was higher when the patients had neutropenia. The longer the duration of severe neutropenia (less than 100/mm3), the higher the susceptibility of febrile episodes was observed.Disseminated intravascular coagulation (DIC) occurred in 21 patients during the follow-up period, 80% of which was related to febrile episodes. The fatality rate for infections complicated by DIC was 82%.