10 0 0 0 OA タバコシバンムシから分離された真菌類
- 著者
- 川上 裕司 清水 一郎 高橋 治男
- 出版者
- 日本衛生動物学会
- 雑誌
- 衛生動物 (ISSN:04247086)
- 巻号頁・発行日
- vol.53, no.4, pp.249-256, 2002-12-15 (Released:2016-08-07)
- 参考文献数
- 29
- 被引用文献数
- 5 6
Fungi (eumycetes) isolated from the body surface of the cigarette beetles, Lasioderma serricorne Fabricius (Coleoptera: Anobiidae), were studied for their effect on food sanitation. The cigarette beetles were captured using a pheromone trap in 30 household kitchens in Tokyo, Saitama and Gunma prefectures from the end of July to the beginning of September, 2000. Each of the 150 captured beetles (5 beetles/house) was individually placed in a sterilization tube, with 1 ml of phosphate buffer. By shaking this tube with a touch mixer, the fungal spores adhering to the body surface of the beetle were separated. This extraction liquid was incubated on agar plates of potato dextrose (PDA), Czapek-Dox and Czapek yeast extract (CYA) at 25℃ and/or 26℃ for 5-7 days. Then, the colony grown on the plate was observed and identified on the basis of morphological characters. Consequently, 17 species of molds and about 5 species of yeasts were isolated from the body surface. Genus Aspergillus (12 isolates, 4 species) and genus Eurotium (8 isolates, 2 species) were predominant, followed by genus Penicillium (6 isolates, 4 species) and genus Arthrinium (5 isolates). Among the isolates of the toxigenic species including Aspergillus ochraceus, A. versicolor and A. fumigatus, ochratoxin A production by A. ochraceus were studied. Consequently, both isolates tested positive for toxin production. Moreover, the body surface of the cigarette beetles captured from the kitchens of 3 houses in Tokyo from the middle of August to the beginning of September 2001 was observed by a scanning electron microscope. Some fungal spores, similar to conidia of genus Aspergillus and genus Penicillium, were seen on the surface of the beetles.
2 0 0 0 去勢後の実験的線維肝におけるエストラジオール作用
- 著者
- 川上 裕司 本堂 朋子 米田 麻子 庄子 健一 清水 一郎 井上 正
- 出版者
- 日本家屋害虫学会
- 雑誌
- 家屋害虫 (ISSN:0912974X)
- 巻号頁・発行日
- vol.26, no.2, pp.135-143, 2004-12-15
- 参考文献数
- 22
- 被引用文献数
- 2
2000年8月~9月に,東京都所在の一般住宅6軒と病院1軒を対象として,フェロモントラップを使ってタバコシバンムシを捕獲した.捕獲した個体から2つの方法で細菌と酵母菌を分離した.一方の個体群は1頭ずつ解剖して消化管を取り出し,滅菌チユーブに移した.もう一方の個体群は1頭ずつ翅を外した腹部のみを滅菌チユーブに移した.検体を入れた滅菌チューブに1mlのリン酸緩衝生理食塩水を加え,タッチミキサーを使って振とうした.この洗浄液を6種類の培地を用いて,好気性または嫌気性培養して細菌と酵母菌を分離した.分離された細菌と酵母菌からDNAを抽出した.そして,SSUrDNAの塩基配列を調べることによって細菌と酵母菌の同定を行った.この結果,14種29株を同定した.この内訳は,グラム陽性菌8種20株,グラム陰性菌4種7株,酵母菌2種2株であった.細菌は7属が分離された.この内訳は,Bacillus cereusを含むBacillus属が4種,Enterobacter agglomeransを含むEnterobacter属が3種で,他の5属は1種ずつだった。病院で採集した個体から抗生物質耐性菌として知られているEnterococcus faecalisが分離されたことは注目すべき結果だった.また,酵母菌はCandida kruseiとRhodotorula rubraであった。
1 0 0 0 <座談会>回顧・毎日新聞の選挙世論調査(上)(世論調査の60年)
- 著者
- 山埜井 乙彦 清水 一郎 越谷 和子
- 出版者
- 公益財団法人 日本世論調査協会
- 雑誌
- 日本世論調査協会報「よろん」 (ISSN:21894531)
- 巻号頁・発行日
- vol.100, pp.44-49, 2007
1 0 0 0 新規経口型抗炎症剤の開発とその薬物徐放特性
1 0 0 0 OA 30cm 望遠鏡による太陽粒状斑の観測
- 著者
- 平山 淳 浜名 茂男 徳家 厚 今井 英樹 清水 一郎 守山 史生 吉田 安徳 佐下橋 市太郎 丹羽 登
- 出版者
- 宇宙航空研究開発機構
- 雑誌
- 宇宙科学研究所報告. 特集 (ISSN:02859920)
- 巻号頁・発行日
- vol.4, pp.93-105, 1982-08
Direct photographs of the solar granulation near the limb were obtained with a 30cm balloon-borne telescope. The observed position on the solar disk was at cosθ=0.7,where θ is the angle between the observer and the normal to the solar surface and the wavelength used was 545nm with a pass band of 40nm. Total half-width (FWHM) of the autocorrelation function of the contrast of the granulation was found to be 780km and 660km in radial and tangential direction to the solar limb, respectively. Although the latter value agrees with the older determination at the disk center, the distance, 2000km, to the minimum value of the auto-correlation function was much larger than those obtained by Leighton at the disk center. Two dimensional power spectrum was obtained and after corrections due to noise spectrum and finite resolution of the optical system, the root-mean-square brightness fluctuation of the granulation was found to be 7.0-7.9%. It is to be noted that the modulation transfer function of the optical system was determined at the partial eclipse of July, 30,1981. Two dimensional power spectrum per unit frequency area (arcsec^2) was fonund to be proportional to exp (-1.9f) where f is the frequency (cycles/arcsec). Further the autocorrelation function as determined from the earlier balloon flight with the use of a 10cm telescope showed a positive maximum around 24∿45Mm, which roughly coincides with the size of the supergranulation.