著者

紺野 勝弘

出版者

公益社団法人 日本農芸化学会

雑誌

化学と生物 (ISSN:0453073X)

巻号頁・発行日

vol.48, no.1, pp.2-4, 2010-01-01 (Released:2011-08-10)

参考文献数

10

著者

紺野 勝弘 Picolo Gisele Gutierrez Vanessa Brigatte Patricia Zambelli Vanessa Camargo Antonio C.M. Cury Yara

出版者

天然有機化合物討論会

雑誌

天然有機化合物討論会講演要旨集

巻号頁・発行日

no.50, pp.409-413, 2008-09-01

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Snakebite accidents by the South American rattlesnake Crotalus durissus terrificus account for 10% of those occurred in Brazil. It induces severe neurological symptoms, but does not induce pain or severe tissue destruction at the site of inoculation, which is in contrast to the most other snakebites. Due to these properties, the crude venom of this snake used to be employed for controlling pain, for example, of cancer. Recent studies using the crude venom experimentally demonstrated that this venom shows antinociceptive effect more potent than morphine. This effect is orally active and long-lasting for 3-5 days, and despite mediated by opioid receptors, it dose not develop peripheral tolerance nor induce physical dependence unlike morphine. These remarkable properties prompted us to purify and chemically characterize the substance responsible for the analgesic effect. Bioassay-guided fractionation led to the isolation of a novel peptide, designated crotalphine, with a sequence of 14 amino acid residues having a single disulfide bond. We report herein the isolation, sequence determination and synthesis of crotalphine. Pharmacological evaluation using synthetic peptide will also be reported.

著者

林 貴昭 尾島 克二 紺野 勝弘 間中 明彦 山口 健太郎 山田 幸子 高山 浩明

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.40, no.11, pp.2932-2936, 1992-11-25 (Released:2008-03-31)

参考文献数

16

被引用文献数

5 4

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A new synthesis of 24-fluoro-1α, 25-dihydroxyvitamin D2 (4a) and its 24-epimer (4b) is described. Starting with 1α, 3β-bis[(tert-butyldimethylsilyl)oxy]-24-norchol-5, 7-dien-23-al (5), a mixture of 4a and 4b was obtained in 3% overall yield in 6 steps. Reversed-phase HPLC cleanly separated the mixture into the two C-24 epimers. The X-ray crystallographic analysis of the 4-phenyl-1, 2, 4-triazoline-3, 5-dione (PTAD) adduct 11b, which was derived from the ester 6, unambiguously determined the configuration at C-24 of this compound. Based on the X-ray analysis, the configuration at C-24 of 4a and 4b was unequivocally determined.

著者

紺野 勝弘 尾島 克二 林 貴昭 高山 浩明

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.40, no.5, pp.1120-1124, 1992-05-25 (Released:2008-03-31)

参考文献数

17

被引用文献数

15 24

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An alternative synthesis of the title compound 2, a highly potent analog of 1α, 25-dihydroxyvitamin D3 (1), is described. Starting with 1α, 3β-bis[(tert-butyldimethylsilyl)oxy]androst-5-ene (8), 2 was obtained in 3.8% total yield through 10 steps. This method provides compound 2 in much higher yield than that reported previously.

著者

篠崎 淳一 数馬 恒平 佐竹 元吉 近藤 一成 紺野 勝弘

出版者

公益社団法人 日本食品衛生学会

雑誌

食品衛生学雑誌 (ISSN:00156426)

巻号頁・発行日

vol.59, no.3, pp.134-140, 2018-06-25 (Released:2018-07-21)

参考文献数

15

被引用文献数

1

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自然毒,特に有毒植物の誤食による食中毒は症状が重篤なことが多く致死率が高いことが特徴である.そのため,原因植物の迅速かつ正確な同定が求められている.本研究では1989~2015年に厚生労働省に報告された植物による食中毒事例を調査した.植物由来食中毒の傾向を鑑みて,発生件数の多い4種(バイケイソウ類,チョウセンアサガオ類,トリカブトおよびスイセン)および死亡事例があるイヌサフランの迅速・簡便な同定法を構築した.PCR-RFLP法を利用した本法は電気泳動像において,有毒植物では2本,食用植物では1本のバンドを検出することで判定できる.また,本法は簡便な操作で,高価な機器を必要とせず,短時間に一義的な結果が得られる.また,調理した試料に対しても適用可能である.

著者

紺野 勝弘 白浜 晴久 松本 毅

出版者

天然有機化合物討論会

雑誌

天然有機化合物討論会講演要旨集

巻号頁・発行日

no.25, pp.421-428, 1982-09-10

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Clitocybe acromelalga is a poisonous mushroom distributed in Japan only. It exhibits unique toxicity. If one takes it accidentaly, one will have intolerable pain in fingers and toes after some days and the pain continues for about a month. We were interested in these remarkable physiological activities. It was difficult to reproduce the above described symptoms in experimental animals, however. So we fractionated various constituents of the mushroom testing the lethal effect on mice. Clitidine was a weakly toxic, a new nucleoside. Clithioneine, an unusual betaine, was nontoxic. From the most toxic fraction, acromelic acid A (ca. 110μg) and acromelic acid B (ca. 40μg) were isolated. We suggest formula 1 and 2 for A and B respectively on the basis of ^1H NMR and UV data.