著者

金内 長司 光岡 知足 山本 脩太郎 瀬賀 利夫

出版者

社団法人日本獣医学会

雑誌

日本獸醫學雜誌 (ISSN:00215295)

巻号頁・発行日

vol.28, no.5, pp.229-236, 1966-10-25

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The authors previously devised an inexpensive germ-free chicken cage that couldbe used for experimetnts of 3 or 4 weeks duratiotx. In this study, cage operations werecarried out from January tltrough December, 1965.The following results were obtained.1. Out of 9 experiments to rear germ-free chicks in 8 cages each, eight were suc..cessful from a bacteriological point of view. The other experiment could not be corn..pleted>bacause of an accident in electrical heating. In one experiment, contaminationwas noticed in all 8 cages within 2 days after hatching.2. For bacteriological exatnination>the specimens were collected from more thanfifty eggs with 18 or 20-day-old embryos at the following sites: inside the egg membrane,on the surface of the embrvo>e?u volk>antd intestinal contents. Bacteria were recoveredfrom only a few sites of these eggs Oll culture media with very sntaJ] colony counts.3. One hundred and fortv-four sterilized CggS with 20-dav-old embrvos introducedin the cages gave an average hatchabilitv of 75%. The uerrrt-free t"ate of chicks wasabout 90% at 2 davs after Itatchinw (Just before feeclin:) and about 7Ocs at 25 cta.s or a.e.4. Conventional chicks reared on a non-sterilized cJiet irt cages showed a highergrowth rate than the 8CFITl-fFCC ChlCkS. Ill .1VCt1&C bOd>z weiwht, the male and fernaleconventional chicks were 40 amxd 30 Q, restcectivelv>heavier than the cour-tterparts or thegerm-free chicks at 25 days of age.5. There was no noticeable difference in the growtln rate at 25 days of age betweengerm-free chicks reared on a commercial diet arnd those on diet L=I37 reportect byRE,YNIERS at al.

著者

飯田 孝 神崎 政子 仲真 晶子 小久保 彌太郎 丸山 務 金内 長司

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.60, no.12, pp.1341-1343, s・v, 1998-12

被引用文献数

12 55

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ヒト, 動物, 食品, 合計31, 127検体におけるL.monocytogenesの検出率はリステリア疾患者100%(39/39), 健康人1.3%(38/2, 970)であり, 動物ではネズミが最も高く6.5%(13/199)であった.市販食品は枝肉より高い汚染率であった.チーズ, 魚介類および魚介類加工品における検出率は市販食肉に比べて低かったが, これらの食品から分離した菌の血清型は, 患者から多く分離される1/2a, 1/2b, 4bの3血清型が96.7%(29/30), 90.0%(9/10), 100%(13/13)と高い割合であった.