著者

Namangala Boniface Hachaambwa Lottie Kajino Kiichi Mweene Aaron S. Hayashida Kyouko Simuunza Martin Simukoko Humphrey Choongo Kennedy Chansa Pamela Lakhi Shabir Moonga Ladslav Chota Amos Ndebe Joseph Nsakashalo-Senkwe Mutale Chizema Elizabeth Kasonka Lackson Sugimoto Chihiro

出版者

BioMed Central

雑誌

Parasites & Vectors (ISSN:17563305)

巻号頁・発行日

vol.5, 2012-12-04

被引用文献数

1 35

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Background: Loop-mediated isothermal amplification (LAMP) is a novel strategy which amplifies DNA with high sensitivity and rapidity under isothermal conditions. In the present study, the performance of the repetitive insertion mobile element (RIME)-LAMP and human serum resistance-associated gene (SRA)-LAMP assays were evaluated using clinical specimens obtained from four male patients from Luangwa and Zambezi valleys in Zambia and Zimbabwe, respectively. Findings: The cases reported in this preliminary communication were all first diagnosed by microscopy, through passive surveillance, and confirmed by both RIME-LAMP and SRA-LAMP. A good correlation between microscopy and LAMP was observed and contributed to staging and successful treatment of patient. RIME-LAMP and SRA-LAMP complimented each other well in all the cases. Conclusions: Both RIME-LAMP and SRA-LAMP were able to detect Trypanosoma brucei rhodesiense DNA in patient blood and CSF and hence confirmed HAT in the parasitaemic patients. Our study indicates that the LAMP technique is a potential tool for HAT diagnosis, staging and may be useful for making therapeutic decisions. However, no statistically significant conclusion may be drawn due to the limited sample size used in the present study. It is thus imperative to conduct a detailed study to further evaluate the potential of LAMP as a bedside diagnostic test for HAT.

著者

Syakalima Michelo CHOONGO Kennedy 中里 幸和 小沼 操 杉本 千尋 坪田 敏男 福士 秀人 吉田 光敏 板垣 匡 安田 準

出版者

社団法人日本獣医学会

雑誌

The journal of veterinary medical science (ISSN:09167250)

巻号頁・発行日

vol.63, no.3, pp.315-318, 2001-03-25

被引用文献数

9

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ザンビア国カフエ川湿原で, 銅鉱山排液が混入するロッキンバー及びブルーラグーン国立公園に生息する野生生物, 重金属被爆がもたらす環境危険因子について食物連鎖解析を行った.ここでみられる食物連鎖因子は, 水, 魚, 植物(草).カフエレチュエ(Kobus Leche Kafuensis)であり, 重金属は, 銅, 亜鉛, マンガン, 鉄である.銅は水:0.03-0.04, 魚3.0-6.0, 草11.0-44.0, レチュエ肝臓:痕跡-199.0であった.亜鉛は水:0.01, 魚32.0-82.0, 草:15.0-21.0.レチュエ肝臓:52.0-138.0であった.マンガンは水:0.15-0.16, 魚:7.0-18.0, 草:51.0-1450, レチュエ肝臓:40.0-53.0であった.鉄は水:0.13-0.14, 魚:26.0-134.0, 草:1766.0-1797.0, レチュエ肝臓:131.0-856.0であった.濃度単位は水がmg/l, その他の試料はmg/kgである.水以外の全ての因子で重金属濃度が高かったが, 毒性は裏づけられなかった.