著者

Yasuko Okamoto Takanori Sakaguchi Yoshito Ikematsu Toshikazu Kanai Kazuhisa Hirayama Hiroaki Tamura Tadataka Hayashi Yoshiro Nishiwaki Hiroyuki Konno Katsunori Aoki

出版者

The University of Tokushima Faculty of Medicine

雑誌

The Journal of Medical Investigation (ISSN:13431420)

巻号頁・発行日

vol.70, no.3.4, pp.325-333, 2023 (Released:2023-11-09)

参考文献数

37

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The effects of early enteral arginine-rich nutrition (EAN) were analyzed among patients undergoing curative-intent total gastrectomy for gastric cancer. There were 19 patients in this prospective study, all randomly assigned to either a parenteral nutrition (PN) group or an EAN group for the first seven days after surgery. The EAN group received 1.8-fold greater arginine (10.1 g/day) compared with the PN group, which was administered through an enteral tube inserted into the jejunal loop. Both groups were provided almost identical amounts of total amino acids (54 g/day), and the total energy was set at 65% of the total requirement (25 kcal/kg/day). No significant differences were observed between the two groups in postoperative complications, length of hospital stay, oral intake, nutritional status, or body weight. The serum arginine profile was similar in the two groups, as it decreased significantly on postoperative day (POD) 1, and gradually returned to preoperative levels by POD 7. The nitrogen balance remained negative until POD 7 in the PN group, but turned neutral at POD 7 in the EAN group. While we could not confirm body weight loss improvement, these results suggested that early arginine-rich enteral nutrition could improve the nitrogen balance after total gastrectomy. J. Med. Invest. 70 : 325-333, August, 2023

著者

Minako Okukawa Takamasa Watanabe Maki Miura Hiroyuki Konno Shigekazu Yano Yoshimune Nonomura

出版者

Japan Oil Chemists' Society

雑誌

Journal of Oleo Science (ISSN:13458957)

巻号頁・発行日

pp.ess19074, (Released:2019-07-10)

被引用文献数

7

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1,2-Alkanediol exhibits antibacterial activity against several bacteria and yeast. However, few studies have reported antimicrobial tests on skin microbiome. Bacterial microbiome on the skin surface include Staphylococcus aureus (S. aureus), which causes rough skin and inflammation in atopic dermatitis and Staphylococcus epidermidis (S. epidermidis), which enhances innate immunity. In this study, the minimal inhibitory concentration (MIC) was evaluated for 1,2-alkanediol comprising 4–12 carbon atoms against S. aureus and S. epidermidis. 1,2-Alkanediol comprising 6–12 carbon atoms exhibited antimicrobial activity against both species of Staphylococcus. The antibacterial activity depended on the alkyl chain length. In addition, the minimum bactericidal concentration (MBC) on agar was evaluated for 1,2-alkanediol comprising 6–12 carbon atoms. 1,2-Octanediol and 1,2-decanediol exhibited significant bactericidal activity.

著者

Takamasa Watanabe Yoshiaki Yamamoto Maki Miura Hiroyuki Konno Shigekazu Yano Yoshimune Nonomura

出版者

Japan Oil Chemists' Society

雑誌

Journal of Oleo Science (ISSN:13458957)

巻号頁・発行日

pp.ess18220, (Released:2019-02-13)

被引用文献数

17

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Bacterial flora on the skin surface contains Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis) which causes rough skin and atopic dermatitis and enhances innate immunity, respectively. In this study, minimum inhibitory concentration (MIC) was evaluated for six saturated fatty acids and two unsaturated fatty acids against S. aureus and S. epidermidis. The antimicrobial behavior in the liquid medium was categorized into three groups. The first was the selective antibacterial activity group comprising myristic acid (C14:0 fatty acid), palmitoleic acid (C16:1 fatty acid), and oleic acid (C18:1 fatty acid) and preferentially displayed antimicrobial activity for S. aureus (group 1). C16:1 fatty acid displayed high antimicrobial activity only for S. aureus. The second was the non-selective antibacterial activity group which displayed antibacterial activity for both Staphylococci (group 2). Caprylic acid (C8:0 fatty acid), capric acid (C10:0 fatty acid), and lauric acid (C12:0 fatty acid) comprised group 2. The third was the non-antibacterial activity group which did not show significant antimicrobial activity (group 3). Bactericidal activities were confirmed for C12:0 fatty acid and C16:1 fatty acid by evaluating the minimum bactericidal concentration (MBC) on the agar medium. C12:0 fatty acid displayed non-selective bactericidal behavior against S. aureus and S. epidermidis when the fatty acid concentration was above 250 μg mL–1. These findings suggest that C16:1 fatty acid has the potential to be used as a detergent in skin care and medical products because it can selectively kill only S. aureus.

著者

Yuitsu Otsuka Koki Sato Shigekazu Yano Haruki Kanno Wasana Suyotha Hiroyuki Konno Koki Makabe Toki Taira

出版者

The Japanese Society of Applied Glycoscience

雑誌

Journal of Applied Glycoscience (ISSN:13447882)

巻号頁・発行日

pp.jag.JAG-2022_0002, (Released:2022-04-14)

被引用文献数

3

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The GH-16 type β-1,3-glucanase (BgluC16MK) gene of Lysobacter sp. MK9-1 was cloned to study its antifungal activities. BgluC16MK displays amino acid sequence similarity with GluC from L. enzymogenes strain N4-7. BgluC16MK includes a signal sequence, a catalytic domain and carbohydrate-binding module family 6-type β-glucan binding domain (B-GBD). The expression of the BgluC16MK gene in Escherichia coli without the signal sequence resulted in antifungal activity at a dose of 0.6–0.8 nmol/disk. However, BgluC16MK displayed antifungal activity at a dose of 0.025 nmol/disk in combination with Chi19MK. Substrate-specific assay revealed that purified BgluC16MK hydrolyzed insoluble curdlan more readily than the soluble substrate. Furthermore, to explore the binding selectivity of B-GBD of BgluC16MK, we constructed a fusion protein (B-GBD-GFP) using the B-GBD and green fluorescent protein. The activity of the fusion protein against various substrates indicates that B-GBD was selective for glucans with β-1,3-linkages. An additional study demonstrated the binding ability of BGBD-GFP to the cell-wall of living fungi, such as T. reesei and Aspergillus oryzae. These findings suggest that BgluC16MK can be utilized to generate antifungal enzyme preparations and that the fusion protein B-GBD-GFP can be used to identify the fungal cell surface structure using β-glucans.

著者

齋藤 路子 沢崎 達夫 今野 裕之 Michiko Saito Tatsuo Sawazaki Hiroyuki Konno 目白大学大学院心理学研究科 目白大学人間学部 目白大学人間学部 Mejiro University Graduate School of Psychology Mejiro University Faculty of Human Sciences Mejiro University Faculty of Human Sciences

雑誌

目白大学心理学研究 = Mejiro journal of psychology (ISSN:13497103)

巻号頁・発行日

vol.(4), pp.101-109, 2008

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本研究の目的は,完全主義と帰属スタイルの関連の検討,および完全主義が抑うつに至るプロセスに関するモデルを構成し,共分散構造分析による検討を行うことであった。大学生444名は完全主義,内的安定的帰属スタイル,抑うつを測定する質問紙に回答した。その結果,完全欲求および高目標設定は成功場面でも失敗場面でも内的安定的帰属と関連する一方で,失敗過敏および行動疑念は失敗場面のみ内的安定的帰属と関連することが示された。共分散構造分析の結果,(a)適応的完全主義が強いほど,対人領域における成功に対して内的安定的帰属をしやすく,ポジティブ感情が強まること,(b)不適応的完全主義が強いほど,対人領域における失敗に対して内的安定的帰属をしやすく,抑うつが強まることが示された。最後に,完全主義が抑うつに至る認知プロセスについて介入方法も交えて議論した。