著者

Jun-ichi Takahashi Shin-ichi Akimoto Eisuke Hasegawa Jun Nakamura

出版者

JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY

雑誌

Applied Entomology and Zoology (ISSN:00036862)

巻号頁・発行日

vol.37, no.3, pp.481-486, 2002 (Released:2003-04-25)

参考文献数

29

被引用文献数

12 14

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Vespa ducalis is a monogynous hornet with an annual life cycle. Its colonies are the smallest in the genus Vespa. Generally, it is thought that the number of queen matings and colony size are positively correlated, so we analyzed the queen mating number and genetic relatedness between workers of V. ducalis using microsatellite DNA markers. We examined foundress queens and 20 workers from each of 20 colonies. All colonies were found to have one queen inseminated by one male. The genetic relatedness between workers was 0.724±0.0029 (mean±SE), which is not significantly different from the expected value of 0.75 for full sisters. This result suggests a possible conflict in male production between queens and workers based on kin selection prediction. Therefore, we performed microsatellite analysis of 400 males from 20 colonies to verify whether males are derived from either queens or from workers. No males could be assigned to workers developed ovaries were not found in a total of 880 mature workers. These results strongly suggest that queens dominate production of males in V. ducalis colonies.

著者

高橋 純一 野村 哲郎 Jun-ichi TAKAHASHI Tetsuro NOMURA 京都産業大学総合生命科学部 京都産業大学総合生命科学部

出版者

京都産業大学先端科学技術研究所

雑誌

京都産業大学先端科学技術研究所所報 = The bulletin of the Research Institute of Advanced Technology Kyoto Sangyo University (ISSN:13473980)

巻号頁・発行日

vol.14, pp.31-40, 2015-07

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ミツバチ科マルハナバチに属するエゾオオマルハナバチBombus hypocrita sapporoensisは、特定外来種であるセイヨウオオマルハナバチの代替花粉交配用昆虫として注目されている。今回我われは、本種の農業利用を進めるための遺伝育種学的解析に必要なマイクロサテライトDNAマーカーの適用性を検討した。120種類のマーカーのうち、57種類が多型解析に利用できることがわかった。さらに8種類のプライマーは、マルチプレックスPCRによる同時解析が可能であることを明らかにした。これらのツールキットを利用して女王蜂の受精嚢内から単離した精子DNAの解析を行ったところ、交配雄蜂の遺伝子型を特定できることがわかった。 The Bumblebee is a eusocial Hymenoptera with an annual life cycle and is often utilized as an agricultural pollinator. We developed an polymorphic microsatellite DNA toolkit for the Japanese bumblebee Bombus hypocrita sapporoensis using multiplex PCR. At 57 of these 120 loci, high allelic variation was observed in 20 individual males. In addition to a method of spermathecae PCR, we developed 8 polymorphic microsatellite DNA toolkit for the B. h. sapporoensis. These results suggest that high-throughput genotyping method can be used to gain more information regarding breeding systems and for more deeply understanding social evolution in the B. h. sapporoensis.