著者

Maiko Saito Minoru Nakayama Kyota Fujita Atsuko Uchida Hiroyuki Yano Satoshi Goto Hitoshi Okazawa Masaki Sone

出版者

The Genetics Society of Japan

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

vol.95, no.6, pp.303-314, 2020-12-01 (Released:2021-03-23)

参考文献数

58

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yata mutants of Drosophila melanogaster exhibit phenotypes including progressive brain shrinkage, developmental abnormalities and shortened lifespan, whereas in mammals, null mutations of the yata ortholog Scyl1 result in motor neuron degeneration. yata mutation also causes defects in the anterograde intracellular trafficking of a subset of proteins including APPL, which is the Drosophila ortholog of mammalian APP, a causative molecule in Alzheimer’s disease. SCYL1 binds and regulates the function of coat protein complex I (COPI) in secretory vesicles. Here, we reveal a role for the Drosophila YATA protein in the proper localization of COPI. Immunohistochemical analyses performed using confocal microscopy and structured illumination microscopy showed that YATA colocalizes with COPI and GM130, a cis-Golgi marker. Analyses using transgenically expressed YATA with a modified N-terminal sequence revealed that the N-terminal portion of YATA is required for the proper subcellular localization of YATA. Analysis using transgenically expressed YATA proteins in which the C-terminal sequence was modified revealed a function for the C-terminal portion of YATA in the subcellular localization of COPI. Notably, when YATA was mislocalized, it also caused the mislocalization of COPI, indicating that YATA plays a role in directing COPI to the proper subcellular site. Moreover, when both YATA and COPI were mislocalized, the staining pattern of GM130 revealed Golgi with abnormal elongated shapes. Thus, our in vivo data indicate that YATA plays a role in the proper subcellular localization of COPI.

著者

Kota Kasahara Hiroki Terazawa Hayato Itaya Satoshi Goto Haruki Nakamura Takuya Takahashi Junichi Higo

出版者

The Biophysical Society of Japan

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

pp.BSJ-2020013, (Released:2020-10-15)

被引用文献数

9

著者

Shuping ZHANG Jinjia ZHOU Dajiang ZHOU Shinji KIMURA Satoshi GOTO

出版者

一般社団法人 電子情報通信学会

雑誌

IEICE Transactions on Electronics (ISSN:09168524)

巻号頁・発行日

vol.E100.C, no.3, pp.223-231, 2017-03-01 (Released:2017-03-01)

参考文献数

31

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In this paper, a hamburger architecture with a 3D stacked reconfigurable memory is proposed for a 4K motion estimation (ME) processor. By positioning the memory dies on both the top and bottom sides of the processor die, the proposed hamburger architecture can reduce the usage of the signal through-silicon via (TSV), and balance the power delivery network and the clock tree of the entire system. It results in 1/3 reduction of the usage of signal TSVs. Moreover, a stacked reconfigurable memory architecture is proposed to reduce the fabrication complexity and further reduce the number of signal TSVs by more than 1/2. The reduction of signal TSVs in the entire design is 71.24%. Finally, we address unique issues that occur in electronic design automation (EDA) tools during 3D large-scale integration (LSI) designs. As a result, a 4K ME processor with 7-die stacking 3D system-on-chip design is implemented. The proposed design can support real time 3840 × 2160 @ 120 fps encoding at 130 MHz with less than 540 mW.

著者

Ning JIANG Jiu XU Satoshi GOTO

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

IEICE TRANSACTIONS on Fundamentals of Electronics, Communications and Computer Sciences (ISSN:09168508)

巻号頁・発行日

vol.E95-A, no.8, pp.1280-1287, 2012-08-01

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In recent years, local pattern based features have attracted increasing interest in object detection and recognition systems. Local Binary Pattern (LBP) feature is widely used in texture classification and face detection. But the original definition of LBP is not suitable for human detection. In this paper, we propose a novel feature named gradient local binary patterns (GLBP) for human detection. In this feature, original 256 local binary patterns are reduced to 56 patterns. These 56 patterns named uniform patterns are used for generating a 56-bin histogram. And gradient value of each pixel is set as the weight which is always same in LBP based features in histogram calculation to computing the values in 56 bins for histogram. Experiments are performed on INRIA dataset, which shows the proposal GLBP feature is discriminative than histogram of orientated gradient (HOG), Semantic Local Binary Patterns (S-LBP) and histogram of template (HOT). In our experiments, the window size is fixed. That means the performance can be improved by boosting methods. And the computation of GLBP feature is parallel, which make it easy for hardware acceleration. These factors make GLBP feature possible for real-time pedestrian detection.