著者

Ryosuke OTSUJI Toshiyuki AMANO Satoshi MATSUO Yuichiro MIYAMATSU Kenta HARA So TOKUNAGA Akira NAKAMIZO

出版者

The Japan Neurosurgical Society

雑誌

NMC Case Report Journal (ISSN:21884226)

巻号頁・発行日

vol.9, pp.151-155, 2022-12-31 (Released:2022-06-15)

参考文献数

28

被引用文献数

1

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Endovascular embolization of the middle meningeal artery (MMA) has been reported as an effective method for treating chronic subdural hematoma (CSDH); however, its preventive effect on CSDH following craniotomy is unknown. We present a case in which MMA embolization was ineffective in preventing CSDH following craniotomy. A 56-year-old man who complained of diplopia was diagnosed with sphenoid ridge meningioma with a 3-cm diameter. MMA embolization prior to the operation and total surgical removal of the tumor were performed. Two months postoperatively, the patient complained of headache and hemiparesis of the left side. CSDH with a 15-mm thickness and a midline shift was observed. MMA embolization before inflammation may not play a role in preventing CSDH development because MMA embolization is considered effective in CSDH because it is associated with the blood supply of neovessels that are newly formed due to inflammation. Therefore, MMA embolization might not be effective in preventing the occurrence of CSDH following craniotomy.

著者

Satoshi Matsuo Kenji Nanya Shunsuke Imanishi Ichiro Honda Eiji Goto

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.UTD-005, (Released:2018-10-20)

被引用文献数

13

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Light quality is an important environmental factor that regulates stem length of the tomato (Solanum lycopersicum). Previously, we showed that the stem length of tomato seedlings grown under red (R) light emitting diodes (LEDs) was significantly longer than that of seedlings grown under blue (B) LEDs or a mixture of B and R LEDs; further, the light intensity of B LED negatively correlated with the stem length. This study aimed to elucidate the mechanism of how B and R lights affect stem elongation. We analyzed the levels of gibberellins (GAs) and the expression of genes associated with their metabolism in tomato seedlings grown under different B and R light conditions. The level of bioactive GA, GA4, was significantly higher in the seedlings grown under R LED than in those grown under other light conditions. In addition, an increase in the B to R light ratio increased the transcript level of the GA inactivation enzyme gene, SlGA2ox7. Moreover, the transcript level of SlGA2ox7 increased with the intensity of B light, and was negatively correlated with the stem length of the seedlings. These results indicated that the B light intensity controlled GA inactivation of the seedlings, and endogenous GA contents may affect stem elongation. Further, we found that the transcript level of the GA biosynthesis enzyme gene, SlGA3ox3, in the seedlings grown under R LED was significantly higher than that under other light conditions. This could be due to depletion of B light and suggests that GA biosynthesis may be involved in the stem elongation of seedlings grown under low B light conditions.

著者

Hiroki Ueno Takeshi Maeda Naoki Katsuyama Yu Katou Satoshi Matsuo Kanako Yano Akira Ando Kaori Nagasuga Mizuki Yamada Shunsuke Imanishi

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-101, (Released:2017-10-11)

被引用文献数

11

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Several Japanese tomato cultivars develop a physiological disorder called leaf marginal necrosis, which occurs in relatively young compound leaves. Although the positions of the observed symptoms differ from those caused by inadequate potassium (K+) supplementation, previous studies have reported a relationship between the reduction of K+ content and the occurrence of this disorder. However, the mechanism of the relationship between K+ deficiency and leaf marginal necrosis remains unstudied. In the present study, the relationship between K+ deficiency in leaflets and leaf marginal necrosis was investigated by cation measurement and gene expression analysis to understand the possible mechanism responsible for the induction of leaf marginal necrosis. First, cation measurement of the two cultivars differing in their symptom intensities showed a trend of K+ reduction in the ‘CF Momotaro J’ cultivar developing leaf marginal necrosis at the tip leaflets positioned under the flowering fruit truss. Next, a comparison between the basal and tip region of the leaflet from four cultivars differing in their symptom intensities revealed that the K+ concentration in tip leaflets was significantly lower in the tip regions compared to the basal region, especially in the two cultivars ‘CF Momotaro J’ and ‘Momotaro grande’, leading to leaf marginal necrosis. The gene expression analysis of the basal and tip regions identified that the expression patterns of jasmonate-related genes were upregulated in the tomato leaflets with low K+ concentration. The gene expression of a leaf senescence marker gene, a homologue of the SAG12 gene of Arabidopsis thaliana, was detected only in the leaf tip region samples with the lowest K+ concentration. Furthermore, ‘CF Momotaro J’ plants cultivated with K+-supplemented medium showed an increase in the K+ concentration, a decrease in the occurrence of leaf marginal necrosis, and down-regulation of the expression of jasmonate-related genes in tip leaflets. These results indicate that tomato leaf marginal necrosis occurs because of K+ starvation in the tip region of leaflets, leading to the activation of jasmonate-induced signal for necrosis.

著者

Nobuhiro Kotoda Satoshi Matsuo Ichiro Honda Kanako Yano Tokurou Shimizu

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-016, (Released:2016-09-27)

被引用文献数

5

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Gibberellins (GAs) are involved in many aspects of plant growth and development in higher plants. In this study, we aimed to characterize Satsuma mandarin (Citrus unshiu Marc.) GA 2-oxidase genes encoding enzymes with GA inactivation activity because the accumulation of active GAs is regulated by the balance between their synthesis and inactivation. We showed that CuGA2ox4, CuGA2ox2/3, and CuGA2ox8 were differentially expressed in various tissues in Satsuma mandarin and that these genes functioned like GA 2-oxidase genes in transgenic Arabidopsis. The expression pattern of CuGA2ox4 resembled that of CuGA20ox1, which was reported previously as a Satsuma mandarin GA 20-oxidase gene, in the reproductive tissues of the adult trees in that both genes were expressed in juice sacs in November and December and peel in December, suggesting that CuGA2ox4 and CuGA20ox1 act in concert to regulate the accumulation of active GAs in fruit at the maturing stage. On the other hand, CuGA2ox2/3 and CuGA2ox8 were more highly expressed in seeds and flower buds, respectively. Further study of GA biosynthetic genes, including GA 2-oxidase genes, would provide insight into the mechanism of flowering, fruit development, seedlessness, biennial bearing, and peel puffins of citrus such as Satsuma mandarin.

著者

Nobuhiro Kotoda Satoshi Matsuo Ichiro Honda Kanako Yano Tokurou Shimizu

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-085, (Released:2015-11-28)

被引用文献数

13

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Satsuma mandarin (Citrus unshiu Marc.) has two GA 20-oxidase genes, CuGA20ox1 and CuGA20ox2, and the genomic sequence for CuGA20ox1 is shorter than that for CuGA20ox2, although the coding region of cDNA for CuGA20ox1 is slightly longer than that for CuGA20ox2. Southern blot analysis revealed that 12 Citrus cultivars examined and trifoliate orange [Poncirus trifoliata (L.) Raf.] have at least two types of GA 20-oxidase genes, such as CuGA20ox1 and CuGA20ox2 genes. CuGA20ox1 and CuGA20ox2 were differentially expressed in various tissues. CuGA20ox1 was expressed in almost all of the tissues investigated with relatively higher expression in vegetative than in reproductive tissues, whereas CuGA20ox2 was specifically expressed in flower buds just before anthesis. These distinct expression patterns of CuGA20ox1 and CuGA20ox2 imply that function of these two genes diverged in the process of evolution. The specific and relatively higher expression of CuGA20ox2 in flower buds would explain why GA-like activity was higher in Satsuma mandarin’s ovaries at anthesis. Transgenic Arabidopsis [Arabidopsis thaliana (L.) Heynh] plants ectopically expressing CuGA20ox1 or CuGA20ox2 were examined to elucidate the function of these two Satsuma mandarin genes. Phenotypic analysis revealed that both CuGA20ox1 and CuGA20ox2 caused elongated inflorescence but did not affect the timing of flowering in transgenic Arabidopsis as compared with wild-type controls. Ectopic expression of CuGA20ox1 and CuGA20ox2 significantly affected the levels of GA24 and GA34 on the non-13-hydroxylation pathway; GA24 decreased and GA34 increased. This observation indicates that both CuGA20ox1 and CuGA20ox2 accelerated the conversion of GA24, a substrate of a GA 20-oxidase, to GA9, a precursor of an active form of GA4. Likewise, on the early-13-hydroxylation pathway, ectopic expression of CuGA20ox1 significantly decreased GA19 and increased GA29 and GA8, inactive metabolites of 2-hydroxylation of GA20 and GA1, respectively, suggesting the activation of this biosynthetic pathway. CuGA20ox2 also had a tendency to activate the early-13-hydroxylation pathway although it increased only GA20 with a statistically significant difference. Taken together, we concluded that CuGA20ox1 and/or CuGA20ox2 activated both the early-13- and non-13-hydroxylation pathways for increasing active GAs, resulting in elongated inflorescences in transgenic Arabidopsis.

著者

Yasushi Kawasaki Satoshi Matsuo Yoshinori Kanayama Koki Kanahama

出版者

園芸学会

雑誌

Journal of the Japanese Society for Horticultural Science (ISSN:18823351)

巻号頁・発行日

pp.MI-001, (Released:2014-09-03)

被引用文献数

4 25

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Low-cost heating is needed to reduce chilling injuries, heating costs, and CO2 emission during greenhouse tomato production. To acquire information about the physiological and morphological effects of root-zone heating, an economical option at low air temperatures, we grew tomato plants on a nutrient film technique hydroponic system in a heated nutrient solution. We investigated the effects of short-term root-zone heating after transplanting and long-term heating until harvest. We measured short-term plant growth, nutrient uptake, root activity (xylem exudation and root respiration rates), root indole-3-acetic acid (IAA) concentration, internal root structure, and long-term fruit weight and dry matter distribution. The minimum root-zone temperature was maintained at 16.6°C, while the minimum air temperature (5.9°C) and the minimum root-zone temperature in the control (5.8°C) were lower than optimal. After 7 days of root-zone heating, root dry weight and relative growth rate increased compared with those of the control, accompanied by increased mineral nutrient uptake and xylem exudation. These changes may explain the increased shoot growth after 21 days of heating. In roots, development of the epidermis and stele, including the xylem, was promoted by heating, in contrast to previous research on root-zone cooling at high air temperature, which promoted xylem-specific development. Although the proportion of dry matter distributed to the fruit was not changed by root-zone heating, individual fruit size and total yield were higher than in the control due to a higher total dry weight in the heating treatment. Our results suggest that root-zone heating is an effective low-cost heating technology at low air temperature because of its effects on root activity, growth, and fruit yield, but that the mechanisms may differ from those in root-zone cooling at high air temperature.