- 著者
- Shigeto Morita So Sugiyama Yoshihiro Nomura Takehiro Masumura Shigeru Satoh
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-007, (Released:2017-02-22)
- 被引用文献数
- 3
2,4-Pyridinedicarboxylic acid (2,4-PDCA) extends the vase life of cut flowers of spray-type carnations by accelerating flower opening as well as retarding senescence. Since 2,4-PDCA can inhibit 2-oxoglutarate-dependent dioxygenases, which include enzymes for gibberellin (GA) biosynthesis and catabolism, we hypothesized that GA might be involved in the enhancing effect of 2,4-PDCA on the flower opening of carnation. In this study, we tested this possibility by examining the changes in gene expression of DELLA protein (GAI), a negative regulator of GA signaling, and GA levels in carnation (Dianthus caryophyllus L. ‘Light Pink Barbara (LPB)’) flowers treated with 2,4-PDCA. We also analyzed the expression of cell expansion-related genes, xyloglucan endotransglucosylase/hydrolase (XTH), and expansin genes as markers of flower opening in the treated flowers. The transcript level of GAI gene was increased, whereas that of expansin was decreased, in petals of the 2,4-PDCA-treated flowers compared to those of the control, which was contrary to the enhancement of flower opening. Our results suggest that the changes in the expression of these genes are not associated with the enhancing effects of 2,4-PDCA. In addition, GA3 content tended to be decreased by 2,4-PDCA treatment in the petals of opening flowers. Flower opening was not accelerated, but rather delayed, by treatment of flower buds with exogenous GA3 and not affected by paclobutrazol, an inhibitor of GA biosynthesis, in ‘LPB’ carnation. These results suggest that endogenous GA is not associated with the enhancement of flower opening by 2,4-PDCA in carnation.
- 著者
- Shigeto Morita So Sugiyama Akira Tateishi Shigeru Satoh
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.MI-127, (Released:2016-05-19)
- 被引用文献数
- 7
Transport of water into cells is mediated by plasma membrane intrinsic protein (PIP) families of aquaporin, which are involved in petal cell expansion during flower opening. In this study, we performed comprehensive characterization of aquaporin family genes and analyzed the expression of PIP genes in petals of opening flowers to examine the role of PIPs in flower opening in the carnation (Dianthus caryophyllus L.). A database search of the genome sequence revealed the existence of 26 aquaporin genes with 8 members of the PIP subfamily in the carnation ‘Francesco’. The expression of all the PIP genes was validated by the existence of expressed sequence tags, and expression analysis by quantitative reverse transcription-PCR showed that DcPIP2;1 and DcPIP1;1 are the two major PIP isoforms expressed in petals of the ‘Pure Red’ carnation. The transcripts of these two genes were also detected abundantly in other floral tissues including the calyx, style, receptacle, and ovary, as well as stems and leaves. The expression of DcPIP2;1 and DcPIP1;1 in petals was maintained at a high level throughout the flower opening process. These data suggest a putative role of these PIPs in petal growth for flower opening.
- 著者
- Shigeru Satoh Yoshihiro Nomura
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.UTD-038, (Released:2018-12-28)
- 被引用文献数
- 3
Pyridinedicarboxylic acid (PDCA) analogs extend the vase life of spray-type (SP) carnation flowers by accelerating flower opening and retarding senescence. Among the PDCA analogs, 2,3-PDCA and 2,4-PDCA are equally and highly effective. In the present study, we characterized the promotion of flower opening by PDCAs and their analogs, pyridinecarboxylic acids. The present results showed that 3-pyridinecarboxylic acid (3-PCA), and its derivative, 3-PCA amide, are most active in accelerating flower opening of ‘Light Pink Barbara’ carnation cut flowers. 3-PCA treatment promoted flower opening in cut flowers of many SP carnation cultivars, that had been stored dry at 2–4°C for 3 weeks and resulted in extension of their display time.
- 著者
- Shigeto Morita So Sugiyama Yoshihiro Nomura Takehiro Masumura Shigeru Satoh
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- vol.86, no.4, pp.519-527, 2017 (Released:2017-10-25)
- 参考文献数
- 35
- 被引用文献数
- 3
2,4-Pyridinedicarboxylic acid (2,4-PDCA) extends the vase life of cut flowers of spray-type carnations by accelerating flower opening as well as retarding senescence. Since 2,4-PDCA can inhibit 2-oxoglutarate-dependent dioxygenases, which include enzymes for gibberellin (GA) biosynthesis and catabolism, we hypothesized that GA might be involved in the enhancing effect of 2,4-PDCA on the flower opening of carnation. In this study, we tested this possibility by examining the changes in gene expression of DELLA protein (GAI), a negative regulator of GA signaling, and GA levels in carnation (Dianthus caryophyllus L. ‘Light Pink Barbara (LPB)’) flowers treated with 2,4-PDCA. We also analyzed the expression of cell expansion-related genes, xyloglucan endotransglucosylase/hydrolase (XTH), and expansin genes as markers of flower opening in the treated flowers. The transcript level of GAI gene was increased, whereas that of expansin was decreased, in petals of the 2,4-PDCA-treated flowers compared to those of the control, which was contrary to the enhancement of flower opening. Our results suggest that the changes in the expression of these genes are not associated with the enhancing effects of 2,4-PDCA. In addition, GA3 content tended to be decreased by 2,4-PDCA treatment in the petals of opening flowers. Flower opening was not accelerated, but rather delayed, by treatment of flower buds with exogenous GA3 and not affected by paclobutrazol, an inhibitor of GA biosynthesis, in ‘LPB’ carnation. These results suggest that endogenous GA is not associated with the enhancement of flower opening by 2,4-PDCA in carnation.
- 著者
- So Sugiyama Shigeru Satoh
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.MI-025, (Released:2015-02-21)
- 被引用文献数
- 7
2,4-Pyridinedicarboxylic acid (2,4-PDCA) was shown to prolong the vase life of cut flowers of spray-type ‘Light Pink Barbara’ (LPB) carnation, mainly due to the reduced ethylene production caused by inhibition of 1-aminocyclopropane-1-carboxylate oxidase in the flowers. In addition, 2,4-PDCA has been suggested to accelerate flower opening in the flowers (Satoh et al., 2014). In the present study, we successfully developed a procedure to evaluate the activity of chemicals to accelerate flower (bud) opening by determining the shortened time (in days) to flower opening. Using this procedure, we could show the activities of several PDCA analogs to accelerate flower opening, in addition to their already-known activity to extend the vase life in cut flowers of ‘LPB’ carnation. Judging from their effectiveness in the acceleration of flower opening and extension of vase life, 2,3-PDCA and 2,4-PDCA were thought to be suitable agents for treatment of the flowers. The present study confirmed that PDCAs accelerate flower opening and retard senescence, which increase the number of open flowers, resulting in extension of the vase life of cut flowers of ‘LPB’ carnation.