文献一覧: Shigeto Morita (著者)

2 0 0 0 OA Gibberellin is not Associated with the Enhancing Effect of 2,4-Pyridinedicarboxylic Acid on Flower Opening of ‘Light Pink Barbara’ Carnation

著者: Shigeto Morita So Sugiyama Yoshihiro Nomura Takehiro Masumura Shigeru Satoh
出版者: 一般社団法人園芸学会
雑誌: The Horticulture Journal (ISSN:21890102)
巻号頁・発行日: pp.OKD-007, (Released:2017-02-22)
被引用文献数: 3

2,4-Pyridinedicarboxylic acid (2,4-PDCA) extends the vase life of cut flowers of spray-type carnations by accelerating flower opening as well as retarding senescence. Since 2,4-PDCA can inhibit 2-oxoglutarate-dependent dioxygenases, which include enzymes for gibberellin (GA) biosynthesis and catabolism, we hypothesized that GA might be involved in the enhancing effect of 2,4-PDCA on the flower opening of carnation. In this study, we tested this possibility by examining the changes in gene expression of DELLA protein (GAI), a negative regulator of GA signaling, and GA levels in carnation (Dianthus caryophyllus L. ‘Light Pink Barbara (LPB)’) flowers treated with 2,4-PDCA. We also analyzed the expression of cell expansion-related genes, xyloglucan endotransglucosylase/hydrolase (XTH), and expansin genes as markers of flower opening in the treated flowers. The transcript level of GAI gene was increased, whereas that of expansin was decreased, in petals of the 2,4-PDCA-treated flowers compared to those of the control, which was contrary to the enhancement of flower opening. Our results suggest that the changes in the expression of these genes are not associated with the enhancing effects of 2,4-PDCA. In addition, GA3 content tended to be decreased by 2,4-PDCA treatment in the petals of opening flowers. Flower opening was not accelerated, but rather delayed, by treatment of flower buds with exogenous GA3 and not affected by paclobutrazol, an inhibitor of GA biosynthesis, in ‘LPB’ carnation. These results suggest that endogenous GA is not associated with the enhancement of flower opening by 2,4-PDCA in carnation.

2017-02-23 17:28:05
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2 0 0 0 OA Identification and Characterization of Plasma Membrane Intrinsic Protein (PIP) Aquaporin Genes in Petals of Opening Carnation Flowers

著者: Shigeto Morita So Sugiyama Akira Tateishi Shigeru Satoh
出版者: 一般社団法人園芸学会
雑誌: The Horticulture Journal (ISSN:21890102)
巻号頁・発行日: pp.MI-127, (Released:2016-05-19)
被引用文献数: 7

Transport of water into cells is mediated by plasma membrane intrinsic protein (PIP) families of aquaporin, which are involved in petal cell expansion during flower opening. In this study, we performed comprehensive characterization of aquaporin family genes and analyzed the expression of PIP genes in petals of opening flowers to examine the role of PIPs in flower opening in the carnation (Dianthus caryophyllus L.). A database search of the genome sequence revealed the existence of 26 aquaporin genes with 8 members of the PIP subfamily in the carnation ‘Francesco’. The expression of all the PIP genes was validated by the existence of expressed sequence tags, and expression analysis by quantitative reverse transcription-PCR showed that DcPIP2;1 and DcPIP1;1 are the two major PIP isoforms expressed in petals of the ‘Pure Red’ carnation. The transcripts of these two genes were also detected abundantly in other floral tissues including the calyx, style, receptacle, and ovary, as well as stems and leaves. The expression of DcPIP2;1 and DcPIP1;1 in petals was maintained at a high level throughout the flower opening process. These data suggest a putative role of these PIPs in petal growth for flower opening.

2016-05-20 00:28:44
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1 0 0 0 OA Gibberellin is not Associated with the Enhancing Effect of 2,4-Pyridinedicarboxylic Acid on Flower Opening of ‘Light Pink Barbara’ Carnation

著者: Shigeto Morita So Sugiyama Yoshihiro Nomura Takehiro Masumura Shigeru Satoh
出版者: The Japanese Society for Horticultural Science
雑誌: The Horticulture Journal (ISSN:21890102)
巻号頁・発行日: vol.86, no.4, pp.519-527, 2017 (Released:2017-10-25)
参考文献数: 35
被引用文献数: 3

2017-12-29 22:53:59
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1 0 0 0 OA Heteroplasmy and expression of mitochondrial genes in alloplasmic and euplasmic wheat

著者: Kanako Kawaura Asuka Saeki Takehiro Masumura Shigeto Morita Yasunari Ogihara
出版者: 日本遺伝学会
雑誌: Genes & Genetic Systems (ISSN:13417568)
巻号頁・発行日: vol.86, no.4, pp.249-255, 2011 (Released:2011-12-29)
参考文献数: 23
被引用文献数: 3 8 2

The plant chondriome confers a complex nature. The atp4 gene (formerly called orf25) of Aegilops crassa (CR) harbors the promoter sequence of the rps7 gene from common wheat (Triticum aestivum cv. Chinese Spring, CS). The rps7 gene of CR has the promoter sequence of CS atp6. The atp6 gene of CR contains an unknown sequence inside of its coding region. Since repeat sequences have been found around the breaking points, these structural alterations are most likely generated through homologous recombination. In this study, PCR analysis was performed to detect structural alterations in each of three lines: euplasmic lines of Ae. crassa, Chinese Spring, and alloplasmic Chinese Spring wheat with the cytoplasm of Ae. crassa ((cr)-CS). We found that each of these lines contained both genotypes, although mitochondrial genotypes of CR in Chinese Spring wheat and CS genotypes in Ae. crassa were still retained as minor fractions (less than 10%). On the other hand, CS mitochondrial gene frequencies in ((cr)-CS) were shown to be ca. 30%. SNP analysis after DNA sequencing of these genes indicated that minor types of all three mitochondrial genes in alloplasmic wheat contained the mitochondrial gene types from pollens. Since the frequencies of paternal mitochondrial gene types in F1 were about 20%, successive backcrossing increased the frequencies of paternal mitochondrial gene types to around 30% in alloplasmic wheat. Expression profiles of these mitochondrial genes were quantitatively analyzed by RT-PCR. Transcripts of paternal mitochondrial gene types were scarcely found. This suggests that minor fractions including paternal mitochondrial gene types are maintained and silenced in the descendants.

2013-01-23 09:34:54
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