文献一覧: Yoshihiro Nomura (著者)

2 0 0 0 OA Gibberellin is not Associated with the Enhancing Effect of 2,4-Pyridinedicarboxylic Acid on Flower Opening of ‘Light Pink Barbara’ Carnation

著者: Shigeto Morita So Sugiyama Yoshihiro Nomura Takehiro Masumura Shigeru Satoh
出版者: 一般社団法人園芸学会
雑誌: The Horticulture Journal (ISSN:21890102)
巻号頁・発行日: pp.OKD-007, (Released:2017-02-22)
被引用文献数: 3

2,4-Pyridinedicarboxylic acid (2,4-PDCA) extends the vase life of cut flowers of spray-type carnations by accelerating flower opening as well as retarding senescence. Since 2,4-PDCA can inhibit 2-oxoglutarate-dependent dioxygenases, which include enzymes for gibberellin (GA) biosynthesis and catabolism, we hypothesized that GA might be involved in the enhancing effect of 2,4-PDCA on the flower opening of carnation. In this study, we tested this possibility by examining the changes in gene expression of DELLA protein (GAI), a negative regulator of GA signaling, and GA levels in carnation (Dianthus caryophyllus L. ‘Light Pink Barbara (LPB)’) flowers treated with 2,4-PDCA. We also analyzed the expression of cell expansion-related genes, xyloglucan endotransglucosylase/hydrolase (XTH), and expansin genes as markers of flower opening in the treated flowers. The transcript level of GAI gene was increased, whereas that of expansin was decreased, in petals of the 2,4-PDCA-treated flowers compared to those of the control, which was contrary to the enhancement of flower opening. Our results suggest that the changes in the expression of these genes are not associated with the enhancing effects of 2,4-PDCA. In addition, GA3 content tended to be decreased by 2,4-PDCA treatment in the petals of opening flowers. Flower opening was not accelerated, but rather delayed, by treatment of flower buds with exogenous GA3 and not affected by paclobutrazol, an inhibitor of GA biosynthesis, in ‘LPB’ carnation. These results suggest that endogenous GA is not associated with the enhancement of flower opening by 2,4-PDCA in carnation.

2017-02-23 17:28:05
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1 0 0 0 OA Backcrossing to an appropriate genetic background improves the birth rate of carbohydrate sulfotransferase 14 gene-deleted mice

著者: Shin SHIMADA Takahiro YOSHIZAWA Yuki TAKAHASHI Yuko NITAHARA-KASAHARA Takashi OKADA Yoshihiro NOMURA Hitoki YAMANAKA Tomoki KOSHO Kiyoshi MATSUMOTO
出版者: Japanese Association for Laboratory Animal Science
雑誌: Experimental Animals (ISSN:13411357)
巻号頁・発行日: vol.69, no.4, pp.407-413, 2020 (Released:2020-11-12)
参考文献数: 19
被引用文献数: 2 3

Ehlers–Danlos syndromes (EDSs) are heterogeneous group of heritable connective tissue disorders characterized by joint and skin hyperextensibility as well as fragility of various organs. Recently, we described a new type of EDS, musculocontractual EDS (mcEDS-CHST14), caused by pathogenic variants of the carbohydrate sulfotransferase 14 (CHST14) gene mutation. B6;129S5-Chst14tm1Lex/Mmucd (B6;129-Chst14 KO) mice are expected to be an animal model of mcEDS-CHST14. However, >90% of B6;129-Chst14 KO homozygous (B6;129-Chst14−/−) mice show perinatal lethality. Therefore, improvement of the birth rate of Chst14−/− mice is needed to clarify the pathophysiology of mcEDS-CHST14 using this animal model. Some B6;129-Chst14−/− embryos had survived at embryonic day 18.5 in utero, suggesting that problems with delivery and/or childcare may cause perinatal lethality. However, in vitro fertilization and egg transfer did not improve the birth rate of the mice. A recent report showed that backcrossing to C57BL/6 strain induces perinatal death of all Chst14−/− mice, suggesting that genetic background influences the birthrate of these mice. In the present study, we performed backcrossing of B6;129-Chst14 KO mice to a BALB/c strain, an inbred strain that shows lower risks of litter loss than C57BL/6 strain. Upon backcrossing 1 to 12 times, the birth rate of Chst14−/− mice was improved with a birth rate of 6.12–18.64%. These results suggest that the genetic background influences the birth rate of Chst14−/− mice. BALB/c congenic Chst14−/− (BALB.Chst14−/−) mice may facilitate investigation of mcEDS-CHST14. Furthermore, backcrossing to an appropriate strain may contribute to optimizing animal experiments.

2022-04-17 22:00:09
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1 0 0 0 OA Promotion of Flower Opening of Spray-type Carnation Cut Flowers by 3-Pyridinecarboxylic Acid, a Simple Analog of Pyridinedicarboxylic Acids (PDCAs) Including 2,3- and 2,4-PDCAs

著者: Shigeru Satoh Yoshihiro Nomura
出版者: The Japanese Society for Horticultural Science
雑誌: The Horticulture Journal (ISSN:21890102)
巻号頁・発行日: pp.UTD-038, (Released:2018-12-28)
被引用文献数: 3

Pyridinedicarboxylic acid (PDCA) analogs extend the vase life of spray-type (SP) carnation flowers by accelerating flower opening and retarding senescence. Among the PDCA analogs, 2,3-PDCA and 2,4-PDCA are equally and highly effective. In the present study, we characterized the promotion of flower opening by PDCAs and their analogs, pyridinecarboxylic acids. The present results showed that 3-pyridinecarboxylic acid (3-PCA), and its derivative, 3-PCA amide, are most active in accelerating flower opening of ‘Light Pink Barbara’ carnation cut flowers. 3-PCA treatment promoted flower opening in cut flowers of many SP carnation cultivars, that had been stored dry at 2–4°C for 3 weeks and resulted in extension of their display time.

2018-12-28 11:10:21
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1 0 0 0 OA Gibberellin is not Associated with the Enhancing Effect of 2,4-Pyridinedicarboxylic Acid on Flower Opening of ‘Light Pink Barbara’ Carnation

著者: Shigeto Morita So Sugiyama Yoshihiro Nomura Takehiro Masumura Shigeru Satoh
出版者: The Japanese Society for Horticultural Science
雑誌: The Horticulture Journal (ISSN:21890102)
巻号頁・発行日: vol.86, no.4, pp.519-527, 2017 (Released:2017-10-25)
参考文献数: 35
被引用文献数: 3

2017-12-29 22:53:59
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