- 著者
- Shigeto Morita So Sugiyama Yoshihiro Nomura Takehiro Masumura Shigeru Satoh
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-007, (Released:2017-02-22)
- 被引用文献数
- 3
2,4-Pyridinedicarboxylic acid (2,4-PDCA) extends the vase life of cut flowers of spray-type carnations by accelerating flower opening as well as retarding senescence. Since 2,4-PDCA can inhibit 2-oxoglutarate-dependent dioxygenases, which include enzymes for gibberellin (GA) biosynthesis and catabolism, we hypothesized that GA might be involved in the enhancing effect of 2,4-PDCA on the flower opening of carnation. In this study, we tested this possibility by examining the changes in gene expression of DELLA protein (GAI), a negative regulator of GA signaling, and GA levels in carnation (Dianthus caryophyllus L. ‘Light Pink Barbara (LPB)’) flowers treated with 2,4-PDCA. We also analyzed the expression of cell expansion-related genes, xyloglucan endotransglucosylase/hydrolase (XTH), and expansin genes as markers of flower opening in the treated flowers. The transcript level of GAI gene was increased, whereas that of expansin was decreased, in petals of the 2,4-PDCA-treated flowers compared to those of the control, which was contrary to the enhancement of flower opening. Our results suggest that the changes in the expression of these genes are not associated with the enhancing effects of 2,4-PDCA. In addition, GA3 content tended to be decreased by 2,4-PDCA treatment in the petals of opening flowers. Flower opening was not accelerated, but rather delayed, by treatment of flower buds with exogenous GA3 and not affected by paclobutrazol, an inhibitor of GA biosynthesis, in ‘LPB’ carnation. These results suggest that endogenous GA is not associated with the enhancement of flower opening by 2,4-PDCA in carnation.
- 著者
- Shigeto Morita So Sugiyama Yoshihiro Nomura Takehiro Masumura Shigeru Satoh
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- vol.86, no.4, pp.519-527, 2017 (Released:2017-10-25)
- 参考文献数
- 35
- 被引用文献数
- 3
2,4-Pyridinedicarboxylic acid (2,4-PDCA) extends the vase life of cut flowers of spray-type carnations by accelerating flower opening as well as retarding senescence. Since 2,4-PDCA can inhibit 2-oxoglutarate-dependent dioxygenases, which include enzymes for gibberellin (GA) biosynthesis and catabolism, we hypothesized that GA might be involved in the enhancing effect of 2,4-PDCA on the flower opening of carnation. In this study, we tested this possibility by examining the changes in gene expression of DELLA protein (GAI), a negative regulator of GA signaling, and GA levels in carnation (Dianthus caryophyllus L. ‘Light Pink Barbara (LPB)’) flowers treated with 2,4-PDCA. We also analyzed the expression of cell expansion-related genes, xyloglucan endotransglucosylase/hydrolase (XTH), and expansin genes as markers of flower opening in the treated flowers. The transcript level of GAI gene was increased, whereas that of expansin was decreased, in petals of the 2,4-PDCA-treated flowers compared to those of the control, which was contrary to the enhancement of flower opening. Our results suggest that the changes in the expression of these genes are not associated with the enhancing effects of 2,4-PDCA. In addition, GA3 content tended to be decreased by 2,4-PDCA treatment in the petals of opening flowers. Flower opening was not accelerated, but rather delayed, by treatment of flower buds with exogenous GA3 and not affected by paclobutrazol, an inhibitor of GA biosynthesis, in ‘LPB’ carnation. These results suggest that endogenous GA is not associated with the enhancement of flower opening by 2,4-PDCA in carnation.
- 著者
- Kanako Kawaura Asuka Saeki Takehiro Masumura Shigeto Morita Yasunari Ogihara
- 出版者
- 日本遺伝学会
- 雑誌
- Genes & Genetic Systems (ISSN:13417568)
- 巻号頁・発行日
- vol.86, no.4, pp.249-255, 2011 (Released:2011-12-29)
- 参考文献数
- 23
- 被引用文献数
- 3 8 2
The plant chondriome confers a complex nature. The atp4 gene (formerly called orf25) of Aegilops crassa (CR) harbors the promoter sequence of the rps7 gene from common wheat (Triticum aestivum cv. Chinese Spring, CS). The rps7 gene of CR has the promoter sequence of CS atp6. The atp6 gene of CR contains an unknown sequence inside of its coding region. Since repeat sequences have been found around the breaking points, these structural alterations are most likely generated through homologous recombination. In this study, PCR analysis was performed to detect structural alterations in each of three lines: euplasmic lines of Ae. crassa, Chinese Spring, and alloplasmic Chinese Spring wheat with the cytoplasm of Ae. crassa ((cr)-CS). We found that each of these lines contained both genotypes, although mitochondrial genotypes of CR in Chinese Spring wheat and CS genotypes in Ae. crassa were still retained as minor fractions (less than 10%). On the other hand, CS mitochondrial gene frequencies in ((cr)-CS) were shown to be ca. 30%. SNP analysis after DNA sequencing of these genes indicated that minor types of all three mitochondrial genes in alloplasmic wheat contained the mitochondrial gene types from pollens. Since the frequencies of paternal mitochondrial gene types in F1 were about 20%, successive backcrossing increased the frequencies of paternal mitochondrial gene types to around 30% in alloplasmic wheat. Expression profiles of these mitochondrial genes were quantitatively analyzed by RT-PCR. Transcripts of paternal mitochondrial gene types were scarcely found. This suggests that minor fractions including paternal mitochondrial gene types are maintained and silenced in the descendants.