- 著者
- Su KIM Islam M. SAADELDIN Woo Jae CHOI Song Jeon LEE Won Wu LEE Bong Han KIM Hyo Jun HAN Du Hee BANG Byeong Chun LEE Goo JANG
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.73, no.11, pp.1453-1457, 2011 (Released:2011-12-01)
- 参考文献数
- 27
- 被引用文献数
- 14 15
Transgenic research on cattle embryos has been developed to date using viral or plasmid DNA delivery systems. In this study, a different gene delivery system, piggybac transposition, was employed to investigate if it can be applied for producing transgenic cattle embryos. Green or red fluorescent proteins (GFP or RFP) were transfected into donor fibroblasts, and then transfected donor cells were reprogrammed in enucleated oocytes through SCNT and developed into pre-implantation stage embryos. GFP was expressed in donor cells and in cloned embryos without any mosaicism. Induction of RFP expression was regulated by doxycycline treatment in donor fibroblasts and pre-implantational stage embryos. In conclusion, this study demonstrated that piggybac transposition could be a mean to deliver genes into bovine somatic cells or embryos for transgenic research.
- 著者
- Ji Hyun Kim Hyo-Cheol Ha Min-Sook Lee Jung-Il Kang Hyun-Su Kim Sang-Yun Lee Kwang-Ho Pyun Insop Shim
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- Biological and Pharmaceutical Bulletin (ISSN:09186158)
- 巻号頁・発行日
- vol.30, no.4, pp.708-714, 2007 (Released:2007-04-01)
- 参考文献数
- 62
- 被引用文献数
- 18 31
We investigated the neuritogenic effects of Tremella fuciformis (TF), which has been valued in traditional Chinese medicine as a remedy with nutritive and tonic actions, on PC12h cells. The cognitive improving effects of TF on scopolamine-induced (2 mg/kg, s.c.) amnesia in rats were also evaluated with using the Morris water maze task and by performing choline acetyltransferase (ChAT) immunohistochemistry. The water extract of TF (0.01—1 μg/ml) promoted neurite outgrowth of the PC12h cells in a dose dependent manner. TF was highly efficient at the concentration range of 0.1—1 μg/ml. Oral daily treatment with TF (100 or 400 mg/kg) for 14 consecutive days significantly reversed the scopolamine-induced deficit in learning and memory, and it alleviated decrease in cholinergic immunoreactivity induced by scopolamine in the medial septum and hippocampus. The results demonstrate that the promotion of neuritogenesis in neuronal culture cells by TF water extract is related with its activity for improving the performance of rats on a spatial learning and memory task. Moreover, the impairments of spatial learning and memory may be attributable to the decrease in activation of the septohippocampal cholinergic system and that TF ameliorated learning and memory deficits partly through its increasing the central cholinergic activity. Therefore, TF could represent a potentially useful agent that is able to improve the function of impaired cognitive processes.
- 著者
- Ji-Hyun JANG Myoung Geun LEE Sang Uk WOO Chung Ok LEE Jin-Kyu YI Duck-Su KIM
- 出版者
- The Japanese Society for Dental Materials and Devices
- 雑誌
- Dental Materials Journal (ISSN:02874547)
- 巻号頁・発行日
- vol.35, no.4, pp.606-612, 2016-07-29 (Released:2016-07-30)
- 参考文献数
- 33
- 被引用文献数
- 33 33
This study compared the dentin bond strength of a new universal adhesive with that of contemporary multi-step dentin adhesives. Six experimental groups were prepared according to the adhesives used and their application modes: Optibond FL (OB), Adper Single Bond Plus (SB), One-Step Plus (OS), Clearfil SE Bond (CS), All-Bond Universal using etch-and-rinse mode (ABE), and AllBond Universal using self-etch mode (ABS). Micro-tensile bond strength (µTBS) and failure mode were evaluated for each group. The bonded interface was analyzed using transmission electron microscopy (TEM). As a result, µTBS of 6 experimental groups was followed as: OB=ABE=SE=ABS>SB>OS group. TEM micrographs of ABE and ABS groups revealed a homogenous adhesive layer formation. In conclusion, a new universal adhesive can make reliable bond to dentin, regardless of the application mode.
- 著者
- RYOYU TAKEDA SHINPEI MORIMOTO KENZO UCHIDA TSUGUHIKO NAKAI MASAJI MIYAMOTO TETSUJI HASHIBA KOHEI YOSHIMITSU KYUNG SU KIM UMEO MIWA
- 出版者
- The Japan Endocrine Society
- 雑誌
- Endocrinologia Japonica (ISSN:00137219)
- 巻号頁・発行日
- vol.26, no.5, pp.541-547, 1979 (Released:2011-01-25)
- 参考文献数
- 15
- 被引用文献数
- 15 24
We describe the natural recovery from the aggravated hypertension, hypokalemia and suppression of the renin-aldosterone axis after the glycyrrhizin discontinuation in two mild hypertensive women aged 71 and 68 years, who had been administered 273 to 546mg glycyrrhizin daily for 1.5 and 6 months, respectively, for the treatment of liver disease. About one month after the glycyrrhizin discontinuation, acceleration of hypertension, hypokalemia and suppression of the renin-aldosterone system still continued in both patients. At this stage, sodium restriction resulted in the normalization of blood pressure with weight loss and the subsequent sodium repletion produced a rapid increase in blood pressure to hypertensive levels observed before sodium restriction, with weight gain. Plasma renin activity and plasma aldosterone were low and did not respond to sodium restriction. Inappropriately excessive amounts of potassium were also excreted in the presence of hypokalemia. About one and a half months later, the improvements of aggravated hypertension, hypokalemia and suppressed renin-aldosterone system gradually occurred in both patients. Sodium restriction performed about three months later in case 2 no longer produced the changes in blood pressure and body weight. Plasma renin activity and plasma aldosterone responded subnormally to sodium restriction.These results demonstrate that both patients had a prolongation of the syndrome resembling primary aldosteronism except the low plasma aldosterone level about one month after the glycyrrhizin discontinuation. The possible mechanisms by which this prolongation was caused are discussed.
- 著者
- Dae Kyong Kim Yong Koo Kang Moo Yeol Lee Kwang-Gill Lee Joo-Hong Yeo Won Bok Lee Yong Sik Kim Sung Su Kim
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Journal of Health Science (ISSN:13449702)
- 巻号頁・発行日
- vol.51, no.3, pp.317-324, 2005 (Released:2005-06-01)
- 参考文献数
- 43
- 被引用文献数
- 8 15
This article was retracted. Please see the retraction notice.
- 著者
- Jury KIM Jiseong HEO Dongbeom JI Min-Su KIM
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.14-0387, (Released:2014-12-25)
- 被引用文献数
- 1 16
The purpose of this study was to quantitatively assess the pupillary light reflex (PLR) in normal and anesthetized dogs using a pupillometer. Eleven dogs (20 eyes) of various breeds were included. PLRs were measured with a handheld pupillometer in dim light before and during anesthesia. Anesthesia was conducted with atropine, xylazine and ketamine. Parameters of pupillometry included neurological pupil index (NPi), pupil size, percent of change (%CH), latency (LAT), constriction velocity (CV), maximum constriction velocity (MCV) and dilation velocity (DV). NPi, %CH, CV and MCV were significantly decreased during anesthesia compared with the pre-anesthesia data. The results suggest that atropine-xylazine-ketamine combination anesthesia depresses the PLR. Additionally, this study demonstrates the feasibility of the use of a pupillometer in dogs.
- 著者
- Jae Hyun BAE Yong Hwan KWON Ye Chan JUNG Ji Mo JUNG Hae Beom LEE Ki Chang LEE Nam Soo KIM Min-Su KIM
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.13-0146, (Released:2013-05-14)
- 被引用文献数
- 4
A 2-year-old male mixed dog was referred to us for further evaluation and treatment of a 4-week-history of oliguria and abdominal distension after a surgical repair of urethral injury. To relieve the urethral stricture, we placed a self-expanding aortic stent graft extension with a partial coverage with an expanded polytetrafluoroethylene (ePTFE). After the placement of the stent, the dog presented with a normal urinary voiding, despite the presence of urinary incontinence. The current case indicates that the ePTFE-covered, self-expanding ASGE is an effective intervention for the treatment of severe urethral stricture in the dog.
- 著者
- Jury KIM Harold L. Jr. SAPP2 Caryn E. PLUMMER2 Dennis E. BROOKS2 Daeyoung KIM Min-Su KIM
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.12-0059, (Released:2012-06-01)
- 被引用文献数
- 3 7
The objective of the study was to examine changes of intraocular pressure (IOP) undergoing anesthesia in Rhesus Macaques (Macaca mulatta) with Laser-induced Ocular Hypertension. Twenty male rhesus macaques (hypertensive glaucoma eye in OD; normal eye in OS) between 6 and 20 years of age were used for the study. The monkeys were anesthetized with ketamine hydrochloride (10 mg/kg intramuscularly) and 1% isoflurane and then IOP in both eyes was measured by a single investigator using a calibrated TonopenTM applanation tonometer (Mentor, Norwell, MA, U.S.A.). The mean IOP with ketamine anesthesia was 36.70 ± 12.04 (right eye: OD) and 15.88 ± 2.84 (left eye: OS). The mean IOP with isoflurane anesthesia was 19.98 ± 6.67 (right eye: OD) and 15.32 ± 2.15 (left eye: OS). Undergoing isoflurane anesthesia, the IOP of OD was significantly decreased. Conclusively, careful examination of IOP is required to prevent unexpected contraindication on glaucoma patient with isoflurane anesthesia.
- 著者
- Duck-Su KIM Sang Hyuk PARK Gi-Woon CHOI Kyoung-Kyu CHOI Sun-Young KIM
- 出版者
- The Japanese Society for Dental Materials and Devices
- 雑誌
- Dental Materials Journal (ISSN:02874547)
- 巻号頁・発行日
- vol.30, no.5, pp.717-722, 2011 (Released:2011-10-08)
- 参考文献数
- 26
- 被引用文献数
- 12 14
The effect of the ethylenediaminetetraacetic acid (EDTA) treatment on the hybrid layer durability of total-etch dentin adhesives was investigated. A flat dentin surface was exposed and treated with 37% phosphoric acid or 0.1M EDTA. Dentin adhesive was applied and a 4 mm thick composite resin was built-up. Twenty 0.9×0.9 mm2 resin-dentin beams were fabricated in each experimental group. Artificial aging with 10% NaClO was performed in half of the experimental groups for 1 hour. A micro-tensile bond strength (μTBS) test was performed and the bonded interface was analyzed with transmission electron microscopy (TEM). The 37% phosphoric acid-treated group showed a lower μTBS after artificial aging, whereas the 0.1M EDTA-treated group did not. TEM analysis revealed collagen fibrils dissolved in the 37% phosphoric acid-treated group but not in the 0.1M EDTA-treated group. Undissolved minerals were observed in the 0.1M EDTA-treated group. In conclusion, a dentin treatment with 0.1M EDTA is effective in improving the hybrid layer durability.