著者

松本 佳代 山本 京子 唐沢 康子 日野 徳子 中村 厚 高橋 真介 荒木 博陽 奥山 茂 町支 臣成 杉野 栄一 日比野 俐 吉本 真 マツモト カヨ ヤマモト キョウコ カラサワ ヤスコ ヒノ ノリコ ナカムラ アツシ タカハシ マサヤシ アラキ ヒロアキ オクヤマ シゲル チョウシ トミナリ スギノ エイイチ ヒビノ サトシ ヨシモト マコト Kayo Matsumoto Kyoko Yamamoto Yasuko Karasawa Noriko Hino Atsushi Nakamura Masayashi Takahashi Hiroaki Araki Shigeru Okuyama Tominari Choshi Eiichi Sugino Satoshi Hibino Makoto Yoshimoto

雑誌

福山大学薬学部研究年報 = Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University

巻号頁・発行日

vol.22, pp.40-41, 2004

著者

Shoji Maehara Manami Kobayashi Mari Kuwada Tominari Choshi Hirofumi Inoue Yuhzou Hieda Takashi Nishiyama Toshiyuki Hata

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.70, no.3, pp.195-198, 2022-03-01 (Released:2022-03-01)

参考文献数

17

被引用文献数

4

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We investigated similar compounds to ebselen and tideglusib, which exhibit strong activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), using Molecular ACCess System (MACCS) keys. Four candidate compounds were identified. One of them, phenyl-benzothiazol-3-one, showed coronavirus-specific 3C-like (3CL) protease inhibitory activity. The results indicated that a similarity score above 0.81 is a good indicator of activity for ebselen-and-tideglusib-like compounds. Subsequently, we simulated the ring-cleavage Michael reaction of ebselen at the Se center, which is responsible for its 3CL protease inhibitory activity, and determined the activation free energy of the reaction. The results showed that reaction simulation is a useful tool for estimating the activity of inhibitory compounds that undergo Michael addition reactions with the relevant cysteine S atom of 3CL proteases.

著者

Nobuyuki Okamura Hidetoshi Miyauchi Tominari Choshi Takashi Ishizu Akira Yagi

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.26, no.5, pp.658-661, 2003 (Released:2003-05-01)

参考文献数

19

被引用文献数

7 9

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A method for semi-micro high-performance liquid chromatography (HPLC) has been established for the simultaneous determination of 3α-hydroxyglycyrrhetic acid and 3-dehydroglycyrrhetic acid together with glycyrrhizin, glycyrrhetic acid and glycyrrhetic acid mono-glucuronide formed by incubation of glycyrrhizin with rat feces. The analysis was accomplished within 25 min with a TSKgel ODS-80TsQA (150×2.0 mm i.d.) column by linear gradient elution using a mobile phase containing aqueous phosphoric acid and acetonitrile at a flow rate of 0.2 ml·min−1, a thermostatic oven at 25 °C, and detection at 254 nm. The detection limits of these compounds were 0.2 pmol per injection (5 μl). The metabolites of glycyrrhizin, by anaerobic or aerobic incubation with rat fecal suspension over 48 h, were determined. Glycyrrhizin was almost completely converted to metabolite glycyrrhetic acid, and metabolites 3α-hydroxyglycyrrhetic acid and 3-dehydroglycyrrhetic acid in negligible amounts in anaerobic conditions. However, the metabolic time courses of 3-dehydroglycyrrhetic acid when incubated in aerobic conditions revealed that it apparently continued increasing during the whole incubation period.

著者

村田 奈津子 藤森 志保 市原 叔立 佐藤 義男 山地 健人 坪井 洋 内田 勝幸 鈴木 啓仁 山田 昌司 根本 英雄 延廣 順子 町支 臣成 日比野 俐 ムラタ ナツコ フジモリ シホ イチハラ ヨシタツ サトウ ヨシオ ヤマジ タケト ツボイ ヒロシ ウチダ マサユキ スズキ ヒロト ヤマダ マサシ ネモト ヒデオ ノブヒロ ジュンコ チョウシ トミナリ ヒビノ サトシ Natsuko Murata Shiho Fujimori Yoshitatu Ichihara Yoshio Sato Taketo Yamaji Hiroshi Tsuboi Masayuki Uchida Hiroto Suzuki Masashi Yamada Hideo Nemoto Junko Nobuhiro Tominari Choshi Satoshi Hibino

雑誌

福山大学薬学部研究年報 = Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University

巻号頁・発行日

vol.25, 2007