著者

Liang Fu Yong Feng Tantan Ren Min Yang Qianting Yang Yi Lin Hui Zeng Jiaohong Zhang Lei Liu Qingyun Li Mengqi He Peize Zhang Haibin Chen Guofang Deng

出版者

International Research and Cooperation Association for Bio & Socio-Sciences Advancement

雑誌

BioScience Trends (ISSN:18817815)

巻号頁・発行日

pp.2022.01476, (Released:2023-01-02)

参考文献数

19

被引用文献数

2

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Mycobacterium tuberculosis (M.tb) infects a quarter of the world's population and may progress to active tuberculosis (ATB). There is no gold standard for diagnosing latent tuberculosis infection (LTBI). Some immunodiagnostic tests are recommended to detect LTBI but can not distinguish ATB from LTBI. The breath test is useful for diagnosing ATB compared to healthy subjects but was never studied for LTBI. This proof-of-concept study (Chinese Clinical Trials Registry number: ChiCTR2200058346) was the first to explore a novel, rapid, and simple LTBI detection method via breath test on high-pressure photon ionization time-of-flight mass spectrometry (HPPI-TOFMS). The case group of LTBI subjects (n = 185) and the control group (n = 250), which included ATB subgroup (n = 121) and healthy control (HC) subgroup (n = 129), were enrolled. The LTBI detection model indicated that a breath test via HPPI-TOFMS could distinguish LTBI from the control with a sensitivity of 80.0% (95% CI: 67.6%, 92.4%) and a specificity of 80.8% (95% CI: 71.8%, 89.9%). Nevertheless, further intensive studies with a larger sample size are required for clinical application.

著者

Shuaibo Huang Ru Ding Yi Lin Zhiqing He Feng Wu Xianliang Dai Yihong Chen Yanping Gui Zhigang Huang Zonggui Wu Chun Liang

出版者

Japan Atherosclerosis Society

雑誌

Journal of Atherosclerosis and Thrombosis (ISSN:13403478)

巻号頁・発行日

vol.23, no.5, pp.632-643, 2016-05-02 (Released:2016-05-02)

参考文献数

59

被引用文献数

5 13

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Aim: Immunologic dysfunction was recently found to be one of the most important mechanisms underlying the initiation and development of atherosclerosis. Thymus involution can contribute to immune disturbance and disequilibrium of T-cell subsets. This study aimed to explore whether recent thymic emigration (RTE) is impaired in patients with coronary artery disease (CAD).Methods: Content of signal-joint T cell receptor excision circles (sj-TREC) in T lymphocytes, a molecular marker of RTE, was assessed among CAD patients and age-matched controls. Monochrome multiplex quantitative PCR method was used to assess the samples' telomere length in order to exclude the potential influence of T cell proliferation on the dilution of sj-TREC. Patients were grouped according to Gensini score (GS) (low, GS <18; intermediate, GS 18–41; high, GS >41). Ordinary logistic regression models were used to determine potential risk factors for CAD and GS tertiles.Results: Average copy numbers of sj-TREC per 106 T lymphocytes among patients with unstable angina, stable angina, and controls were 726±429, 1213±465, and 1795±838, respectively (P<0.001). However, there was no significant difference in telomere length among groups. Moreover, the content of sj-TREC in the high GS group was most significantly reduced than the low GS group (P<0.001). Multivariate logistic regression analysis revealed that lower sj-TREC was independently associated with the progression of CAD (OR=0.44, P<0.001) and higher GS (OR=0.4, P<0.001).Conclusion: Impaired RTE could be partly responsible for CAD development. Mechanisms may be involved in the disturbance of T lymphocyte compartment and interruption of maintained immune tolerance resulting from thymus involution.

著者

Nan-Yin CHEN Hsi-Huai LAI Tai-Hao HSU Fang-Yi LIN Jian-Zhi CHEN Hui-Chen LO

出版者

Japan Society for Bioscience, Biotechnology, and Agrochemistry

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.72, no.5, pp.1283-1289, 2008-05-23 (Released:2008-05-23)

参考文献数

18

被引用文献数

16

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Tremella mesenterica (TM) is a common food and folk medicine widely used in several Asian countries as a tonic for the lungs. In the present study, we compared the effects of extracellular polysaccharides (EPS), intracellular polysaccharides (IPS), and ethanol extract (EE) of Tremella mesenterica on the induction of apoptosis into human lung carcinoma A549 epithelial cells. The EE, but not the EPS or the IPS, almost completely inhibited the growth of A549 cells. The results of Annexin V-FITC/PI staining and flow cytometric analysis indicated that the percentage of Annexin V+/PI− cells in EE-treated cells increased to 32.8%. The results of further investigation showed a disruption of mitochondrial transmembrane potential (ΔΨm), the production of reactive oxygen species (ROS), and the activation of caspase-3 protein in EE-treated cells. These findings suggest that EE can decrease cell viability and induce apoptosis in A549 cell lines by activating a mitochondrial pathway.

著者

Shih-Yi Lin Cheng-Li Lin Wu-Huei Hsu I-Kuan Wang Chiz-Chung Chang Chiu-Ching Huang Chia-Hung Kao Shu-Hui Liu Fung-Chang Sung

出版者

(公社)日本産業衛生学会

雑誌

Journal of Occupational Health (ISSN:13419145)

巻号頁・発行日

vol.55, no.3, pp.158-166, 2013 (Released:2013-10-04)

参考文献数

36

被引用文献数

1 8

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Background: Physicians are frequently studied as a population that experiences extremely high stress, burnout, and prolonged working hours that might harm one's health. However, they have sound medical knowledge and have easy access to medical resources. We studied the incidence of cancer among Taiwanese physicians using a nationwide cohort study design. Methods: Data were obtained from the National Health Insurance (NHI) system in Taiwan. The physician cohort contained 22,309 physicians, and each physician was randomly frequency-matched according to age and sex with 4 people from the general population. Results: The overall incidence ratio of cancer was 27% lower in the physician cohort than in the nonphysician comparison cohort (33.9 vs. 46.5 people per 10,000 person-years, crude hazard ratio (HR)=0.73, 95% CI=0.70, 0.76). The adjusted HR was 0.78 (95% CI=0.72, 0.84). Female physicians experienced a higher incidence rate ratio of overall cancer, compared to male physicians (crude HR=1.17, 95% CI=1.03, 1.33 vs. crude HR=0.70, 95% CI=0.67, 0.74, respectively). Physicians were at a significantly higher risk of thyroid cancer (HR 1.75, 95% CI=1.14, 2.68), prostate cancer (HR=1.54, 95% CI=1.21, 1.97), breast cancer (HR=1.45, 95% CI=1.00, 2.09), and non-cervical gynecological cancer (HR=4.03, 95% CI=1.77, 9.17), compared with the general population. Conclusions: Physicians are at lower overall risk of cancer than the general population, apart from cancer of the thyroid, prostate, breast, and non-cervical gynecological cancer.(J Occup Health 2013; 55: 158–166)

著者

Medel Manuel L Zulueta Shu-Yi Lin Shang-Cheng Hung

出版者

FCCA(Forum: Carbohydrates Coming of Age)

雑誌

Trends in Glycoscience and Glycotechnology (ISSN:09157352)

巻号頁・発行日

vol.25, no.144, pp.141-158, 2013 (Released:2013-07-25)

参考文献数

79

被引用文献数

7 13

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ヘパリンおよびヘパラン硫酸 (HS) は、複雑な骨格を持つ多糖であり、多くのタンパク質の活性を媒介・調節している。それらのタンパク質は、多様な硫酸化パターンで修飾された繰返し2糖骨格を持つ糖鎖と相互作用する。相互作用に関する分子レベルの詳細な知見は、新しい形態の診断薬や治療薬の開発に役立つと期待される。ヘパリンおよびHS骨格オリゴ糖の化学合成は、構造活性相関研究のための構造が明確な試料の入手を容易とするため、非常に重要である。しかしながら、化学合成においてもいくつかの課題に直面することが多い。いくつかの課題とは、例えば、希少なL-イドース/L-イズロン酸誘導体の入手、グルコサミンのグリコシル化反応におけるα-立体選択性、糖鎖の伸長法、位置選択的な硫酸基の導入を含む保護基の選択とその取り扱い、天然のヘパリンおよびHS鎖に相当する充分な多様性を持った化合物群の効率的な調製である。本総説は上記の課題に取り組んだ私達の成果に焦点をおきながら、これまで研究されてきた解決策を簡潔にまとめる。さらに、合成した糖鎖を用いた生物学的アッセイの結果についても簡単に述べる。

著者

Shih-Yi Lin Cheng-Li Lin Wu-Huei Hsu I-Kuan Wang Chiz-Chung Chang Chiu-Chin Huang Chia-Hung Kao Shu-Hui Liu

出版者

(公社)日本産業衛生学会

雑誌

Journal of Occupational Health (ISSN:13419145)

巻号頁・発行日

pp.12-0263-OA, (Released:2013-04-09)

被引用文献数

1 8

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Background: Physicians are frequently studied as a population that experiences extremely high stress, burnout, and prolonged working hours that might harm one’s health. However, they have sound medical knowledge and have easy access to medical resources. We studied the incidence of cancer among Taiwanese physicians using a nationwide cohort study design. Methods: Data were obtained from the National Health Insurance (NHI) system in Taiwan. The physician cohort contained 22,309 physicians, and each physician was randomly frequency-matched according to age and sex with 4 people from the general population. Results: The overall incidence ratio of cancer was 27% lower in the physician cohort than in the nonphysician comparison cohort (33.9 vs. 46.5 people per 10,000 person-years, crude hazard ratio (HR) = 0.73, 95% CI = 0.70, 0.76). The adjusted HR was 0.78 (95% CI = 0.72, 0.84). Female physicians experienced a higher incidence rate ratio of overall cancer, compared to male physicians (crude HR = 1.17, 95% CI = 1.03, 1.33 vs crude HR = 0.70, 95% CI = 0.67, 0.74, respectively). Physicians were at a significantly higher risk of thyroid cancer (HR 1.75, 95% CI = 1.14, 2.68), prostate cancer (HR = 1.54, 95% CI = 1.21, 1.97), breast cancer (HR = 1.45, 95% CI = 1.00, 2.09), and non-cervical gynecological cancer (HR = 4.03, 95% CI = 1.77, 9.17), compared with the general population. Conclusion: Physicians are at lower overall risk of cancer than the general population, apart from cancer of the thyroid, prostate, breast, and non-cervical gynecological cancer.