著者

Iwao Shimomura Mitsuhiro Abe Yu Li Kenji Tsushima Seiichiro Sakao Nobuhiro Tanabe Masatomi Ikusaka Koichiro Tatsumi

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

vol.58, no.7, pp.965-968, 2019-04-01 (Released:2019-04-01)

参考文献数

15

被引用文献数

7 8

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The patient was a 71-year-old man with severe idiopathic pulmonary fibrosis (IPF) and who demonstrated a slow deterioration of his respiratory condition. After nintedanib administration, his forced vital capacity and chest high-resolution computed tomography (HRCT) findings were stable, but his dyspnea on exertion were worsened. He was diagnosed with pulmonary hypertension (PH) by right heart catheterization (mean pulmonary arterial pressure: 30 mmHg). In this case, we suspected that nintedanib caused his PH, as his IPF had not progressed.

著者

Yu Li Shuqing Ma Ling Yang Xiaoqiong Zhen Dan Qiao

出版者

Meteorological Society of Japan

雑誌

SOLA (ISSN:13496476)

巻号頁・発行日

pp.2020-042, (Released:2020-11-23)

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Array weather radar (AWR), a kind of distributed phased array radar, includes at least three Phased Array Front Ends (PAFE). Every three PAFEs are grouped for synchronous scanning to ensure that the Data Time Difference (DTD) at the same point in the fine detection area is less than 2 s. This paper adopts the multiple-Doppler wind synthesis method to synthesize the wind field of the small-DTD AWR data and discusses the sensitivity analysis on the wind fields under the different DTDs. The analysis experiment includes the wind fields under two types of precipitation systems. One is a convective precipitation system with unstable airflow, and the other is a non-convective precipitation system with stable airflow. The statistical analysis showed that, due to the advection effect and intrinsic evolution of the precipitation systems, the bigger the DTD is, the bigger the wind field error is. When the DTD is greater than 24 s, the wind field obtained by the convective precipitation system has more errors. When the DTD is greater than 84 s, the wind field obtained by the non-convective precipitation system has more errors.

著者

Yu Li Shuqing Ma Ling Yang Xiaoqiong Zhen Dan Qiao

出版者

Meteorological Society of Japan

雑誌

SOLA (ISSN:13496476)

巻号頁・発行日

vol.16, pp.252-258, 2020 (Released:2020-12-23)

参考文献数

28

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Array weather radar (AWR), a kind of distributed phased array radar, includes at least three Phased Array Front Ends (PAFE). Every three PAFEs are grouped for synchronous scanning to ensure that the Data Time Difference (DTD) at the same point in the fine detection area is less than 2 s. This paper adopts the multiple-Doppler wind synthesis method to synthesize the wind field of the small-DTD AWR data and discusses the sensitivity analysis on the wind fields under the different DTDs. The analysis experiment includes the wind fields under two types of precipitation systems. One is a convective precipitation system with unstable airflow, and the other is a non-convective precipitation system with stable airflow. The statistical analysis showed that, due to the advection effect and intrinsic evolution of the precipitation systems, the bigger the DTD is, the bigger the wind field error is. When the DTD is greater than 24 s, the wind field obtained by the convective precipitation system has more errors. When the DTD is greater than 84 s, the wind field obtained by the non-convective precipitation system has more errors.

著者

Jiyuan SI Ranran MENG Peng GAO Feifei HUI Yu LI Xianhu LIU Bin YANG

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.18-0089, (Released:2018-10-23)

被引用文献数

12

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Percutaneous coronary intervention (PCI) is main treatment for acute coronary syndrome (ACS). However, restenosis caused by PCI-induced injury influences the outcome of patients. Linagliptin, a dipeptidyl peptidase-4 (DPP-4) inhibitor, has been reported to ameliorate intimal hyperplasia post vascular injury. The underlying mechanisms by which linagliptin protects against balloon injury are unclear and require to be explored. Herein, Wistar rats with carotid artery balloon injury were given 1, 2 or 3 mg/kg/day linagliprin for 6 weeks. We found that linagliptin attenuated vascular injury-mediated neointima formation in rats without affecting body weight and blood glucose levels. ELISA results indicated that linagliptin significantly reduced overproduction of cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 post balloon injury. By detecting the level of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), we found that linagliptin prevented balloon injury-induced oxidative stress. Additionally, linagliptin decreased the level of Kelch ECH-associating protein 1 (KEAP1) compared with injury group. Results of Western blots and electrophoretic mobility shift assay (EMSA) demonstrated that linagliptin augmented nuclear accumulation of nuclear factor-E2-related factor 2 (NRF2) and its binding ability to target genes in rats with balloon injury. Moreover, heme oxygenase-1 (HO-1) and NAD(P)H quinine oxidoreductase 1 (NQO1), two downstream targets of NRF2, were further up-regulated after linagliptin treatment compared with injury group. In conclusion, our data suggest that linagliptin protects carotid artery from balloon injury-induced neointima formation and activates the NRF2 antioxidant pathway.

著者

Can-Zhao Liu Xiang-Yu Li Ren-Hong Du Min Gao Ming-Ming Ma Fei-Ya Li Er-Wen Huang Hong-Shuo Sun Guan-Lei Wang Yong-Yuan Guan

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-16-0793, (Released:2016-10-19)

参考文献数

41

被引用文献数

13

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Background:Previous research has demonstrated that ClC-3 is responsible for volume-regulated Cl–current (ICl.vol) in vascular smooth muscle cells (VSMCs). However, it is still not clear whether and how ClC-3 is transported to cell membranes, resulting in alteration ofICl.vol.Methods and Results:Volume-regulated chloride current (ICl.vol) was recorded by whole-cell patch clamp recording, and Western blotting and co-immunoprecipitation were performed to examine protein expression and protein-protein interaction. Live cell imaging was used to observe ClC-3 transporting. The results showed that an overexpression of endophilin A2 could increaseICl.vol, while endophilin A2 knockdown decreasedICl.vol. In addition, the SH3 domain of endophilin A2 mediated its interaction with ClC-3 and promotes ClC-3 transportation from the cytoplasm to cell membranes. The regulation of ClC-3 channel activity was also verified in basilar arterial smooth muscle cells (BASMCs) isolated from endophilin A2 transgenic mice. Moreover, endophilin A2 increase VSMCs proliferation induced by endothelin-1 or hypo-osmolarity.Conclusions:The present study identified endophilin A2 as a ClC-3 channel partner, which serves as a new ClC-3 trafficking insight in regulatingICl.volin VSMCs. This study provides a new mechanism by which endophilin A2 regulates ClC-3 channel activity, and sheds light on how ClC-3 is transported to cell membranes to play its critical role as a chloride channel in VSMCs function, which may be involved in cardiovascular diseases.

著者

Yu Li Hailong Yu Liangmin Li Chunyan Song Xiaodong Shang Qi Tan Lujun Zhang Qiaozhen Li

出版者

The Mycological Society of Japan

雑誌

Mycoscience (ISSN:13403540)

巻号頁・発行日

pp.MYC580, (Released:2022-08-11)

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Crossbreeding is the most commonly used method in breeding of Lentinula edodes, however low fruiting rate of the hybrids has always caused troubles and barriers for breeders. An early screening method of the fruiting ability could make the breeding work more efficient. In this paper, a rapid and high-throughput laccase activity detection method based on agar diffusion principle was developed. In this way, we investigated the constitutive and inducible extracellular laccase activity of 36 strains in a breeding population of L. edodes on different media and performed a correlation analysis with fruiting ability of these strains. The results showed the laccase activity of mycelium cultured in non-induced medium for 8 d could be used as an early screening index to judge whether it had fruiting ability at the later stage. Early rapid and simple screening method for hybrid populations was established based on laccase activity characteristics of mycelia. 127 strains from another 5 different hybrid populations were used to verify the early screening method. From the validation results, the early screening method was effective, but the appropriate screening threshold was needed to select according to the cross population, which would greatly to improve the breeding efficiency of L. edodes.

著者

An-Yun GUO Kwok-Sui LEUNG Ming-Fai SIU Jiang-Hui QIN Simon Kwoon-Ho CHOW Ling QIN Chi-yu LI Wing-Hoi CHEUNG

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.15-0025, (Released:2015-07-17)

被引用文献数

2 47

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Sarcopenia is an age-related systemic syndrome with progressive deterioration in skeletal muscle functions and loss in mass. Although the senescence-accelerated mouse P8 (SAMP8) was reported valid for muscular ageing research, there was no report on the details such as sarcopenia onset time. Therefore, this study was to investigate the change of muscle mass, structure and functions during the development of sarcopenia. Besides the average life span, muscle mass, structural and functional measurements were also studied. Male SAMP8 animals were examined at month 6, 7, 8, 9, and 10, in which the right gastrocnemius was isolated and tested for ex vivo contractile properties and fatigability while the contralateral one was harvested for muscle fiber cross-sectional area (FCSA) and typing assessments. Results showed that the peak of muscle mass appeared at month 7 and the onset of contractility decline was observed from month 8. Compared with month 8, most of the functional parameters at month 10 decreased significantly. Structurally, muscle fiber type IIA made up the largest proportion of the gastrocnemius, and the fiber size was found to peak at month 8. Based on the altered muscle mass, structural and functional outcomes, it was concluded that the onset of sarcopenia in SAMP8 animals was at month 8. SAMP8 animals at month 8 should be at pre-sarcopenia stage while month 10 at sarcopenia stage. It is confirmed that SAMP8 mouse can be used in sarcopenia research with established time line in this study.