著者

Naito Hiroyuki Nakamura Amane Inoue Maki

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental animals (ISSN:13411357)

巻号頁・発行日

vol.47, no.2, pp.89-96, 1998-04

参考文献数

39

被引用文献数

4 11

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The ontogenetic changes in responsiveness to benzodiazepine receptor ligands on ultrasonic vocalizations in rat pups from the age of day 3 to day 12 were evaluated. Rat pups, while separated from their dam and littermates and placed in a cold environment, emit ultrasonic vocalizations. These ultrasonic calls became attenuated dose-dependently in number and power after administration of the anxiolytic diazepam (0.25-1.0 mg/kg, sc), but the inhibitory effect of diazepam at the highest dose was less on day 6 and day 9. Moreover, type 1 benzodiazepine receptor ligands, Ro16-6028 and Ro23-0364 (0.5-2.0 mg/kg, sc), also dose-dependently attenuated the ultrasonic vocalizations 30-60 min after injection. The inhibitory effects of these drugs became more pronounced with the increasing age of the pup, and they were equivalent on day 12 to those in adult rats. These results suggest that different ontogenetic changes in development of two subtypes of central benzodiazepine receptors of pups might be related in the psychopharmacological mediation of the ultrasonic vocalization.<br>

著者

曲渕 輝夫 輿水 馨 藤原 公策

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.26, no.2, pp.123-129, 1977-04-25 (Released:2010-08-25)

参考文献数

12

被引用文献数

1 4

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1975年の2月から4月にかけて, 都内某研究所で集団発生したゴールデンハムスターの多数の死亡をともなうwet tailの原因を究明するため, 病因・病理学的検索, 伝達試験を行った。剖検された11匹中10匹に出血性腸炎が, 5匹の肝には, 針頭大の淡黄色または乳白色の壊死病変が認められ, 腸および肝の塗抹標本に, Tyzzer菌と思われる桿菌が多数認められた。発症ハムスターの腸管乳剤を健康ハムスターに経口投与すると, 10~14日で自然例と同様の症状を発現死亡し, 腎および肝の塗抹標本に多数のTyzzer菌が認められた。汚染床敷を入れたケージを54日間室温に放置した後, 健康ハムスターを入れると, 5~19日目までの間にwet tailを発現死亡し, 腸および肝の壊死病変部には, 多数のTyzzer菌が認められた。この事実からwet tailの集団発生に, Tyzzer菌の芽胞が重要な役割を演じていることが示唆された。

著者

野村 達次

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物彙報 (ISSN:00075124)

巻号頁・発行日

vol.1, no.2, pp.11-12, 1952-03-31 (Released:2010-08-25)

著者

武田 満

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.8, no.4, pp.104-106, 1959-07-30 (Released:2010-08-25)

参考文献数

3

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ss系マウスの生時に乳子数を4匹 (2: 2) に制限し, 容積の異る4種の飼育箱に飼育して生産能力として, 成長・性成熟及び産子能力を観察した成績は次の如くである。1) 飼育密度の低いほど成長はよい。2) ♀の膣開口日令においては差はなかつたが, 受胎日令では飼育密度が低いほど長い傾向があつた。♂の精巣下降日令では差は見られなかつた。3) 産子数・産子体重及び生時体重の夫々と飼育密度の間には特に明かな関係は見られなかつた。

著者

青山 美子 植田 康志 栗田 源彦 大橋 秀法 鳥潟 親雄 前島 一淑

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals

巻号頁・発行日

vol.41, no.4, pp.421-435, 1992

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高周波電磁界の生体に及ぼす影響を検索することを目的として, 高周波電磁界の発生装置をウサギ耳介に装着し, 赤外線サーモグラフィによる皮膚表面温度, レーザードップラー血流計による皮膚血流量, ビデオマクロスコープによる細動静脈血管径の変化を測定した。周波数9MHzの高周波電磁界15分間照射によって, 局所の細動脈血管の拡張は認められなかったが, 明らかな皮膚表面温度の持続的な上昇, ならびに, 細静脈血管の拡張と血流量の増加傾向が観察され, 微小循環の亢進が確認された。又, モルモットに対する高周波電磁界の有害作用 (周波数9MHz, 1日8時間×30日間照射) は, 一般行動, 飼料摂取量, 体重, 臓器重量, 血液生化学値, 剖検所見, 組織学的所見では認められなかった。

著者

東家 一雄 木村 通郎

出版者

公益社団法人 日本実験動物学会

雑誌

日本疾患モデル学会記録 (ISSN:09188991)

巻号頁・発行日

vol.11, pp.60-60, 1995-08-15 (Released:2010-08-25)

著者

大島 章子 伊藤 宗之 工藤 達也

出版者

公益社団法人 日本実験動物学会

雑誌

日本疾患モデル動物研究会記録 (ISSN:09112057)

巻号頁・発行日

vol.7, pp.52-52, 1991-08-15 (Released:2010-08-25)

参考文献数

2

著者

Kei MATSUNO Setsunosuke IHARA

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.64, no.3, pp.323-332, 2015 (Released:2015-08-01)

参考文献数

16

被引用文献数

3

著者

Yunyun TIAN Li TAO Siriguleng ZHAO Dapeng TAI Dongjun LIU Pengxia LIU

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.15-0031, (Released:2015-11-27)

被引用文献数

15

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Mesenchymal stem cells (MSCs) are one of the most promising cell populations for tissue engineering and regenerative medicine. Of utmost importance to MSC research is identification of MSC sources that are easily obtainable and stable. Several studies have shown that MSCs can be isolated from amniotic fluid. The sheep is one of the main types of farm animal, and it has many biophysical and biochemical similarities to humans. Here, we obtained MSCs from ovine amniotic fluid and determined the expansion capacity, surface and intracellular marker expression, karyotype, and multilineage differentiation ability of these ovine amniotic fluid mesenchymal stem cells (oAF-MSCs). Moreover, expression levels of differentiation markers were measured using reverse transcription-qPCR (RT-qPCR). Our phenotypic analysis shows that the isolated oAF-MSCs are indeed MSCs.

著者

水谷 誠 茅野 和夫 梅沢 英彦 倉益 茂実

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.23, no.2, pp.59-61, 1974 (Released:2010-08-25)

参考文献数

11

被引用文献数

9

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1.白色の羽毛でおおわれ目・耳の周囲, 頭・背・尾部ならびに副翼部 (副翼羽, 覆翼羽) に散在的に野性型羽毛をもつ羽装―panda―は遺伝形質である。2.その形質は常染色体上の劣性遺伝子によって発現すると思われる。

著者

An-Yun GUO Kwok-Sui LEUNG Ming-Fai SIU Jiang-Hui QIN Simon Kwoon-Ho CHOW Ling QIN Chi-yu LI Wing-Hoi CHEUNG

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.15-0025, (Released:2015-07-17)

被引用文献数

2 47

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Sarcopenia is an age-related systemic syndrome with progressive deterioration in skeletal muscle functions and loss in mass. Although the senescence-accelerated mouse P8 (SAMP8) was reported valid for muscular ageing research, there was no report on the details such as sarcopenia onset time. Therefore, this study was to investigate the change of muscle mass, structure and functions during the development of sarcopenia. Besides the average life span, muscle mass, structural and functional measurements were also studied. Male SAMP8 animals were examined at month 6, 7, 8, 9, and 10, in which the right gastrocnemius was isolated and tested for ex vivo contractile properties and fatigability while the contralateral one was harvested for muscle fiber cross-sectional area (FCSA) and typing assessments. Results showed that the peak of muscle mass appeared at month 7 and the onset of contractility decline was observed from month 8. Compared with month 8, most of the functional parameters at month 10 decreased significantly. Structurally, muscle fiber type IIA made up the largest proportion of the gastrocnemius, and the fiber size was found to peak at month 8. Based on the altered muscle mass, structural and functional outcomes, it was concluded that the onset of sarcopenia in SAMP8 animals was at month 8. SAMP8 animals at month 8 should be at pre-sarcopenia stage while month 10 at sarcopenia stage. It is confirmed that SAMP8 mouse can be used in sarcopenia research with established time line in this study.

著者

吉木 淳 俣賀 宣子 深間内 文彦 日下部 守昭

出版者

公益社団法人 日本実験動物学会

雑誌

日本疾患モデル学会記録 (ISSN:09188991)

巻号頁・発行日

vol.11, pp.54-54, 1995-08-15 (Released:2010-08-25)

参考文献数

2

著者

Kenji SAKUMA Susumu HAYASHI Yoshiyuki YASAKA Hiroto NISHIJIMA Hisakage FUNABASHI Masayoshi HAYASHI Hideaki MATSUOKA Mikako SAITO

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.62, no.2, pp.101-107, 2013 (Released:2013-04-25)

参考文献数

16

被引用文献数

4

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In order to provide healthy experimental animals, it is important to find and remove animals that have been accidentally exposed to various stresses during breeding. This study focuses mouse health-care management. Here we used human olfaction and gas chromatography-mass spectrometry to assess odor intensity and determine the concentrations of odor components. The feces were collected from mice that were exposed to 4 different stresses (no bedding chips, shaking, fasting, and movement restriction). These stresses caused a change in odor intensity as assessed by 6 panelists. Seventeen components were identified as dominant components in the odor that was emitted from feces. The concentration of each compound was converted to relative values versus its odor threshold levels in order to select ones effective for the quality of the odor. As a result, 12 selected components were found to be a useful set for the recognition of mice bred under different stress conditions. The present results may provide useful information for the development of standard fecal odor materials that may be used for the training of mouse care personnel.

著者

Francesco FAZIO Stefania CASELLA Claudia GIANNETTO Elisabetta GIUDICE Giuseppe PICCIONE

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.14-0032, (Released:2014-08-05)

被引用文献数

2 18

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Haptoglobin (Hp), serum amyloid A (SAA), C-reactive protein (CRP), white blood cells (WBC), reactive oxygen metabolites (ROMs), the antioxidant barrier (Oxy-adsorbent) and thiol groups of plasma compounds (SHp) were measured in ten dogs that had been transported a distance of about 230 km within 2 h (experimental group) and in ten dogs that had not been subjected to road transportation (control group). Blood was collected via cephalic venipuncture before road transportation (T0), after road transportation (T1), and more than 6 (T6) and 24 (T24) hours after road transportation in the experimental group (Group A) and at the same time points in the control group (Group B). The GLM (general linear model) Repeated Measures procedure showed a significant difference between the two groups (P<0.0001) and a significant rise (P<0.0001) in the concentrations of Hp, SAA, CRP, WBC, ROMs, Oxy-adsorbent and SHp after road transportation in Group A, underlining that physiological and homeostatic mechanisms are modified differently at various sampling times.

著者

Ruixia Zeng YiBo Zhang Peng Du

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.63, no.1, pp.73-78, 2014 (Released:2014-02-07)

参考文献数

19

被引用文献数

3 18

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Melanocortin 4 receptor (MC4R), which is associated with inherited human obesity, is involoved in food intake and body weight of mammals. To study the relationships between MC4R gene polymorphism and body weight in Beagle dogs, we detected and compared the nucleotide sequence of the whole coding region and 3′- and 5′- flanking regions of the dog MC4R gene (1214 bp). In 120 Beagle dogs, two SNPs (A420C, C895T) were identified and their relation with body weight was analyzed with RFLP-PCR method. The results showed that the SNP at A420C was significantly associated with canine body weight trait when it changed amino acid 101 of the MC4R protein from asparagine to threonine, while canine body weight variations were significant in female dogs when MC4R nonsense mutation at C895T. It suggested that the two SNPs might affect the MC4R gene’s function which was relative to body weight in Beagle dogs. Therefore, MC4R was a candidate gene for selecting different size dogs with the MC4R SNPs (A420C, C895T) being potentially valuable as a genetic marker.

著者

Takehiro Ochi Ippei Nishiura Mitsuyoshi Tatsumi Yoshimi Hirano Kouichi Yahagi Yasuhiro Sakurai Yuji Sudo Hironari Koyama Yuichi Hagita Yoshikatsu Fujimoto Shinji Kitamura Hideki Hashimoto Tomoya Nakamura Asobi Yamada Masayoshi Tanimoto Noriko Nishina

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.62, no.4, pp.329-332, 2013 (Released:2013-10-31)

参考文献数

15

被引用文献数

1 12

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Here, to determine the effects of transport stress on blood parameters in dogs, we investigated the changes in hematologic and serum chemical parameters in healthy beagle dogs transported from Beijing, China, to Osaka, Japan, to obtain the background data. Only the activity of serum alkaline phosphatase increased clearly upon arrival, a change attributed to transport stress, but the activity gradually reduced afterward. No marked changes in levels of other blood parameters were noted. Our findings here suggest that alkaline phosphatase is a useful tool for studying transport stress.

著者

Shizuko NAGAO Masanori KUGITA Daisuke YOSHIHARA Tamio YAMAGUCHI

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.61, no.5, pp.477-488, 2012 (Released:2012-10-25)

参考文献数

81

被引用文献数

13 64

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Polycystic kidney disease (PKD) is a hereditary disorder with abnormal cellular proliferation, fluid accumulation in numerous cysts, remodeling of extracellular matrix, inflammation, and fibrosis in the kidney and liver. The two major types of PKD show autosomal dominant (ADPKD) or autosomal recessive inheritance (ARPKD). ADPKD is one of the most common genetic diseases, with an incidence of 1:500–1,000. Approximately 50% of patients with ADPKD develop end-stage renal disease (ESRD) by the age of 60. On the other hand, ARPKD is relatively rare, with an incidence of approximately 1:20,000–40,000. ARPKD is diagnosed early in life, often prenatally. The gene products responsible for ADPKD and ARPKD distribute in primary cilia and are thought to control intercellular Ca2+. Two types of animal model of PKD have been established: spontaneous hereditary models identified by the typical manifestations of PKD and gene-engineered models established by modification of human orthologous genes. Both types of animal models are used to study the mechanism of cystogenesis and efficacy of medical treatments. In PKD progression, critical roles of signaling pathways including MAPK, mTOR, and PPAR-γ have been discovered with these models. Therefore, experimental animal models are indispensable for investigating molecular mechanisms of PKD onset and progression as well as potential therapeutic treatments.

著者

Tohru KIMURA

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.45, no.1, pp.1-13, 1996 (Released:2003-12-23)

参考文献数

36

被引用文献数

5 10

著者

Hiroshi Masuya

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.61, no.4, pp.365-373, 2012 (Released:2012-07-26)

参考文献数

37

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A huge amount of experimental data from past studies has played a vital role in the development of new knowledge and technologies in biomedical science. The importance of computational technologies for the reuse of data, data integration, and knowledge discoveries has also increased, providing means of processing large amounts of data. In recent years, information technologies related to “ontologies” have played more significant roles in the standardization, integration, and knowledge representation of biomedical information. This review paper outlines the history of data integration in biomedical science and its recent trends in relation to the field of experimental animal science.

著者

Toyoyuki Takada Toshihiko Shiroishi

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.61, no.4, pp.375-388, 2012 (Released:2012-07-26)

参考文献数

68

被引用文献数

4 6 4

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Mammalian quantitative traits that are observed at the whole-body level, such as body weight and length and blood biochemical parameters, are determined by the cooperative effects of multiple genetic and epigenetic factors as well as environmental factors. This complexity has hampered the genetic analysis of quantitative traits. To overcome this difficulty, we have established a full set of consomic mouse strains, also known as chromosome substitution strains, by replacing every chromosome of the classical inbred strain C57BL/6J with its counterpart from the Japanese wild-mouse-derived inbred strain MSM/Ms. The core components of the genomes of these two strains originated from different mouse subspecies. The inter-subspecific large-genome divergence and phenotypic differences between the two strains allowed the identification of genetic determinants for many quantitative traits by comprehensive phenotype screening. For some quantitative traits, the genetic determinants could be dissected into multiple chromosomes, thereby reflecting strain differences between C57BL/6J and MSM/Ms and their simple additive effects on the background of the consomic host strain. For other quantitative traits, the measured values of some consomic strains often far exceeded the range of the two parental strains, which suggests that nonadditive genetic interactions occur among multiple genes located on the substituted MSM/Ms chromosomes and the consomic host chromosomes. Thus, the inter-subspecific consomic strains are unique tools that can be used to identify both additive and nonadditive genetic effects on quantitative complex traits.