著者

阿部 仁一郎 八木 欣平

出版者

Osaka Urban Living and Health Association

雑誌

生活衛生 (ISSN:05824176)

巻号頁・発行日

vol.49, no.3, pp.168-171, 2005 (Released:2005-06-07)

参考文献数

10

被引用文献数

2

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Anisakids are parasitic nematodes infecting fish and mammals. It is well recognized that human infection with this parasite, called anisakiasis, occurs by ingestion of raw or undercooked fish. In Japan, there are estimated to be over 1,000 cases of this infection annually. In 1999, “The Food Sanitation Law Enforcement Regulation” was partly amended, and anisakiasis was newly added to the causative agents of food poisoning. Identification of anisakid larvae has been performed by light microscopy, but it is impossible to accurately identify the worms morphologically when only small portions of the worm are available. In the present study, we showed the usefulness of PCR-based methods for identification of anisakid species using several species of anisakid larvae from Pacific cod. The larvae identified morphologically as Anisakis simplex or Contracaecum osculatum were found also to be positive for PCR amplification with only species-specific primers. In addition, the larvae of Pseudoterranova decipiens, frequently found in anisakiasis in Japan, were positive for amplification with only the P. decipiens-specific primers designed for the study. PCR using species-specific primers is thus concluded to be a useful tool for identification of anisakid larvae when morphological identification is impossible.

著者

孝口 裕一 山野 公明 八木 欣平 奥 祐三郎 松本 淳 浦口 宏二

出版者

北海道立衛生研究所

雑誌

基盤研究(C)

巻号頁・発行日

2013-04-01

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イヌの抗多包条虫ワクチンの開発は将来的に飼いイヌからヒトへ、あるいは終宿主動物の感染率を長期的に下げる有力な手段になる可能性がある。一方、イヌに感染と駆虫を繰返すと部分的な感染抵抗性を示すという報告があり、再感染によって誘導される虫体排除機序を解明することはワクチン開発を行う上で重要であると考えられる。本研究ではイヌに多包条虫を繰返し実験感染させ、従来の糞便検査に加え、分子生物的な解析を行うことにより主要な虫体排除機序の一端を明らかにした。また、繰り返し感染により感染抵抗性を獲得したイヌの虫体排除能は短期的に消失せず、イヌの抗多包条虫ワクチン開発の可能性を裏付けた。