著者

村田 修 板倉 壮太 山本 眞司 服部 亘宏 倉田 道雄 太田 博巳 升間 主計

出版者

Japanese Society for Aquaculture Science

雑誌

水産増殖 (ISSN:03714217)

巻号頁・発行日

vol.65, no.1, pp.93-95, 2017-03-20 (Released:2018-03-20)

参考文献数

11

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In order to breed a faster-growing grouper, the longtooth grouper, Epinephelus bruneus (LG), was hybridized with the giant grouper, E. lanceolatus (GG). Eggs from a female LG that was injected with HCG were inseminated by the fresh sperm of LG and cryopreserved sperm of GG obtained from a fish in Malaysia. Normal hatching rate was 8.8% (LGGG) and 18.6% (LG), respectively. The LGGG and LG larvae were raised for 61 days after hatching; the survival and mean size were 17.5, 42.0% and 63.6±11.3, 36.2±8.7 mm, respectively. This new hybrid grouper would be a promising-breed for aquaculture.

著者

太田 博巳 楠田 聡 工藤 智

出版者

日本水産學會

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.61, no.1, pp.7-12, 1995-01-25

参考文献数

23

被引用文献数

6 16

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Anadromous shishamo smelt <i>Spirinchus lanceolatus</i> returns to rivers a few days before spawing. Fish were caught in the mouth of rivers in Hokkaido, Japan. Artifical insemination in the smelt by the dry method is difficult because the semen collected through abdominal pressure is scanty. First, we compared sperm motility between ejaculated semen spermatozoa and spermatozoa in testicular semen, and verified the insignificant differences in the duration of sperm motility and percent motility of them. Secondly, we examined the motility of tesicular spermatuzoa in various artificial solutions in order to determine the adequate constitution of testicular semen diluent. The spermatozoa were motile in NaCl and mannitol solutions at osmolalities between 0 to 400mOsm/kg. In KCl solution, they were motile at 200mOsm/kg and below, but the motility was remarkably lower than that in NaCl solution. The duration and number of moving spermatozoa decreased as the KCl concentration in the artificial testicular fluid increased, and motility was almost inhibited when the KCl concentration was over 25m_M. In this case, the concentration of K⁺ was approximately twice as high as those of testicular fluid (12.5m_M), obtained by centrifugation of testicuar semen, or seminal plasma (11.0m_M). It was concluded that artificial testicular fluid containing 25 m_M KCl is an efficient testicular semen diluent for the shishamo smelt.

著者

太田 博巳 神力 義仁 本間 正男

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.52, no.4, pp.609-611, 1986-04-25 (Released:2008-02-29)

参考文献数

10

被引用文献数

4 6

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In the case of artificial fertilization practiced in salmonids, rinsing of unfertilized eggs with an isotonic solution (ISER), consisting of 154.7mM NaCl, 3.2mM KCl and 2.3mM CaCl2, has been widely used to avoid contamination of the yolk from broken eggs which often causes infertility of the spermatozoa. Experiments were carried out in the masu salmon Onchorhynchus masou to determine the effect of osmolality and ionic composition of the solution on sperm motility. In ISER, duration of sperm motility was about 6 and 2.5-3 times longer than that in distilled water and isotonic NaCl or CaCl2 solutions, respectively. The maximum duration of sperm motility was obtained in the isotonic condition (300mOsm/kg) when the semen was dilated with solutions of the same ionic composition as ISER. A complete lack of either NaCl or CaCl2 from the isotonic solution resulted in a significant decrease in sperm motility, but that of KCl did not affect the motility. Duration of sperm motility was prolonged successfully in varying mix-tures of NaCl and CaCl2 of the constant osmalality at 300mOsm/kg in the range from 1 to 50mM CaCl2. It is concluded that the isotonic solution of NaCl with 1-30mM CaCl2 is fully efficient as a sperm diluent for the masu salmon, and that the addition of KCl is not necessarily essential for the diluent.

著者

太田 博巳

出版者

日本魚類学会

雑誌

魚類学雑誌 (ISSN:00215090)

巻号頁・発行日

vol.30, no.4, pp.404-411, 1984-02-15 (Released:2010-06-28)

参考文献数

23

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ニシン卵は強い粘着性を示す。この粘性物質の起源と排卵された卵の卵膜中での所在部位を明らかにする目的で, 排卵前後の卵濾胞および排卵された卵の卵膜を電顕的に観察した。排卵前の濾胞の顆粒膜細胞中には, メチレンブルーに濃染され, 種々の電子密度を示す直径2μm以下の顆粒が多数存在した.また, この顆粒は卵門細胞とその周囲の顆粒膜細胞には存在しなかった.これに対して排卵後の濾胞の顆粒膜細胞では顆粒が消失しており, 卵門部位を除く卵膜表面に電子密度の高い薄層が新たに認められ, この薄層は強い粘性を示した.また, 卵門部位の卵膜表面には粘着物質を欠き, これとは異なる物質が観察された。以上の観察結果より, ニシン卵の粘着物質は, 排卵前濾胞の卵門域を除く顆粒膜細胞中に存在する顆粒に起源し, その顆粒は排卵直前に卵膜表面に分泌されることが明らかとなった。