著者
前田 有美 横山 典子 高橋 康輝 土居 達也 松元 圭太郎 上野 裕文 久野 譜也
出版者
一般社団法人日本体力医学会
雑誌
体力科学 (ISSN:0039906X)
巻号頁・発行日
vol.56, no.2, pp.269-278, 2007-04-01 (Released:2007-05-25)
参考文献数
43

The purpose of this study was to investigate the effects of combined resistance training and aerobic training with protein intake after resistance training on body composition in obese middle-aged women. The subjects were 42 middle-aged women (age : 56.5±4.3 years, BMI : 26.6±2.3 kg/m2), who were classified into three supplementation groups: Protein group (PG), Isocalorie placebo group (IG), and Noncalorie placebo group (CG) by Double Blind Randomized Trial. The training program consisted of combined resistance training and aerobic training for 10 weeks, 5 times per week: twice a week in a university training room and 3 times per week at home. The subjects took the nutrient supplement immediately after each resistance training session. We measured body weight (Bw), body fat volume (Bf) by bioelectrical impedance analysis (BIA), and cross-sectional area (CSA) of muscle of the thigh extensor group (Te), flexor group (Tf), and psoas major (Pm) by magnetic resonance imaging (MRI) before and after the 10-week training period. Irrespective of group, Bw and Bf showed a significant decrease after the training period compared to before (p<0.001), and the CSA of Te muscle showed a significant increase after the training period (p<0.01). Moreover, the interaction of time×group was accepted in the CSA of Pm muscle (p<0.05), and the increase in muscle volume for PG was the highest increase among the three supplementation groups (p<0.05)(PG : 2.1±0.8 cm2, IG : 1.3±1.0 cm2, CG : 1.3±0.9 cm2). These results suggest that combined training in middle-aged obese women improves body composition, and resistance training with protein intake may increase the CSA of muscle of psoas major.
著者
竹下 温子 松元 圭太郎 立石 百合恵 森中 房枝
雑誌
鹿児島純心女子大学看護栄養学部紀要 = Bulletin of Faculty of Nursing and Nutrition Kagoshima Immaculate Heart University
巻号頁・発行日
vol.15, pp.55-60, 2011-07

昨今,日本の教育の中で,食育が重要視され,小さいころからの食生活の育成に力を入れているが,その食育の一環として,地産地消と地場の活性をテーマに鹿児島県薩摩川内市の甑島で非常に豊漁である「きびなご」と,薩摩川内市の食材を使った特産品を作ろうと,鹿児島県薩摩川内市にある医療法人九十九会 関小児科医院の管理栄養士さんが立ち上がり,川内きびなご鮨という,鹿児島の郷土料理である酒寿司をアレンジした特産品を考案した。この「川内きびなご鮨」は「食&アグリ・マッチングフェア」コンテストに入賞し,高い評価を得た一品である。 我々はそのきびなご鮨の製造工程のひとつである,きびなごの昆布締めの保存日数(発酵過程)における味の変化について研究依頼を受け,食品の味を決める遊離アミノ酸の測定と発酵過程における微生物の変化について調べることを目的とし,16S rRNA遺伝子による菌の同定を行っている。本研究は,その目的の中の一つとして,きびなごにもともと存在していると考えられる海洋性の菌について,保存日数における菌変化の有無を菌数およびコロニー観察・グラム染色にて比較・検討した結果を報告する。 保存日数における菌の変化を調べるため,味付けをせず,食塩水に浸漬後,真空保存したきびなご(サンプル①),調味後真空し1週間冷蔵保存したもの(サンプル②),調味後真空し2日間冷蔵保存後,チルドにて60日間保存したもの(サンプル③),調味後真空し1晩冷蔵保存後,チルドにて90日間保存したもの(サンプル④)の4サンプルを提供して頂き,段階希釈後,一般海洋性細菌用培地であるZobell培地にて菌の培養を行った。 その結果,①は100CFU/mlの菌が増殖し,④は面白いことに希釈倍数の高い10-4のみに30万CFU/mlの菌が増殖した。何かの影響を受け,希釈倍率が高くなってから菌が増殖してくることもあるため,このプレートからも50株釣り菌した。④については海洋性の菌でない可能性もあり,発酵食品に非常に多く存在する乳酸菌の可能性も示唆されたため,GYP白亜寒天培地にて刺針による培養を行った。その結果,非常に弱いが,クリアゾーンを形成しており,酸を作り出す菌であることがわかった。②,③については,Zobell培地に菌を観察することができなかった。標準寒天培地による生菌数については,保存期間が長いものほど,菌が増殖していることがわかった。 真空後のチルド保存が90日の④は試食にて非常に美味しいと評判のあった昆布締めであり,生菌数や海洋性の菌数からみても一番菌が増殖していた。次に,60日間チルド保存の③についても生菌数は非常に多かったが,海洋性の菌は増殖されてこなかった。これらのことより,海洋性の菌以外のものが優勢的に増えてしまった③より,海洋性の菌も増殖することができる環境下であった④の方が旨味や風味が増していると考えられ,海洋性の菌が水産食品の発酵に重要な役割を与えている可能性が示唆された。Dietary education has been regarded as important, and lays emphasis on upbringing of child's eating habits in recent years in Japan. Under the theme of local production for local consumption and local promotion, a plan to make a special product, used Kibinago (Spratelloides gracilis) that was able to capture it abundantly on Koshiki-Island and other cooking ingredients in Satsuma-Sendai city, was carried out as a part of the dietary education."Sende Kibinago Sushi"arranged the "Sake Zushi"which was local cooking of Kagoshima, was developed by a registered dietitian in Satsuma-Sendai city as the special product, and won prizes in food contests and got high evaluation in Kagoshima. We are investigating that the effect of saving day of Kibinago, sandwiched between sheets of kelp that is one in process of manufacture of the Kibinago-sushi, on change of the taste. In this study, we investigated the change of the number of marine bacteria in four samples; vacuum-packed Kibinago without seasoning (sample①), vacuum-packed seasoning Kibinago after 1-week cold storage (sample②), vacuum-packed seasoning Kibinago after 2-day cold and 60-day chilled storages (sample③), vacuum-packed seasoning Kibinago after 1-night cold and 90-day chilled storages (sample④), with Zobell nutrient medium for general marine bacteria. The colonies of general marine bacteria cultured with the Zobell medium were observed in sample①and sample④(100 and 300,000 cfu/mL, respectively). No colony was observed in the Zobell medium of sample②and③. The colonies of sample④were observed only in the most diluted sample (dilution ratio:10-5). On the other hand, the viable cell number cultured with standard agar nutrient medium was increased with the extension of the save days. Fifty colonies in the Zobell medium of sample ④ were picked up and cultured in glucose-yeast extract-peptone (GYP) chalk agar medium. All colonies cultured in the GYP medium formed clear zone slightly. The result suggested that the marine bacteria in sample④have acid production capacity. The bacteria identification by 16s ribosomal RNA gene analysis has been not finished, therefore, it was attempted to speculate the bacterial strain based on a classification by colony's shape in reference to previous reports identified marine bacteria in "Syuto (dish of pickled skipjack tuna entrails)" by 16s ribosomal RNA gene analysis and classification by colony's shape. The bacteria in sample④were speculated to belong to the genus Staphylococcus. The Kibinago sandwiched between sheets of kelp of sample④obtained highest evaluation by the tasting. The bacteria in the genus Staphylococcus were reported to be concerned with formation of Umami and fragrance, therefore it was considered to be indispensable to marine fermented foods. Therefore, it was speculated that the marine bacteria in sample ④is concerned with formation of Kibinago-sushi's taste. However, further investigations are needed to identify the marine bacteria in sample④and to confirm the mechanism of the effect of the marine bacteria on the taste of Kibinago-sushi.