2 0 0 0 OA 2 ガガイモ科及びナス科植物のステロイドとその細胞分化誘導活性(口頭発表の部)
- 著者
- 黒柳 正典 梅原 薫 柴田 和利 砂山 玲子 遠藤 深春 佐藤 裕子 白須 直美 上野 明
- 出版者
- 天然有機化合物討論会
- 雑誌
- 天然有機化合物討論会講演要旨集
- 巻号頁・発行日
- no.36, pp.9-16, 1994-09-20
Although much attention has been given to cell differentiation inducers as new types of anti-tumore agent, only a few studies has been reported on differentiation inducers from plant sorces. Therefore, we have searched for naturally occouring substances which induce differnciation of leukemia cells. From Condurango Cortex (Marsdenia condurango) (Asclepidaceae) and Periplocae Cortex (Periploca sepium B.) (Asclepidaceae), many kids of pregnan derivatives and some cardenolides. From withania (Withania somnifera) (Solanaceae) (using Indian market withania and withania cultivated at the medicinal plant garden of this university), more than thirty kinds of withanolides were isolated. From Physalis alkekengi (Solanaceae), physalin and neophysalin derivatives were isolated. Structural elucidation of these 60 kinds of steroid derivatives were carried out by means of spectral methods, especially using NMR spectroscopy including H-H COSY, H-C COSY, HMBC, NOE technics. These steroidal derivatives were tested on cell differentiatio inducing activity against mouse myeloid leukemia (M1) cells. Many kinds steroid derivatives showed the activity. Of these active compounds, some kinds of withanolids, 27, 33, 34 and 35 having 4β-hydroxy-5β,6β-epoxy-2-en-1-one structure of AB ring, showed potent differentiation inducing activity.
1 0 0 0 OA 廃棄される柿の蔕の天然資源としての可能性に関する研究
吃逆の治療に使われる柿蔕(Kaki Calyx)のラット脳脊髄液中神経伝達物質に及ぼす影響を検討したところ、GABAの変動は観察されなかったが、アスパラギン酸やグリシン、更に、ドパミンの代謝物であるDOPACやHVA濃度に影響を及ぼすことが示唆された。今後、これらを指標として、日本産の柿の蔕から有効成分の探索を行う予定である。また、柿蔕液の治療効果を処方の異なる施設で後ろ向きに調査したところ、化学療法の施行により発症する吃逆の治療に、高濃度の柿蔕液の服用が適している可能性が示唆されたため、前向き調査によって確認する予定である。