著者

針山 孝彦 河崎 秀陽 高久 康春

出版者

一般社団法人 日本昆虫学会

雑誌

昆蟲.ニューシリーズ (ISSN:13438794)

巻号頁・発行日

vol.23, no.4, pp.145-155, 2020-12-25 (Released:2021-10-21)

参考文献数

26

著者

針山 孝彦 高久 康春 鈴木 浩司 石井 大佑 下村 政嗣

出版者

公益社団法人 日本表面科学会

雑誌

表面科学 (ISSN:03885321)

巻号頁・発行日

vol.36, no.4, pp.201-206, 2015-04-10 (Released:2015-04-21)

参考文献数

8

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Scanning electron microscopy (SEM) has become an essential tool for observing the biological materials, however, various complex procedures preclude observation of living organisms to date. Here we present a new method to observe living organisms by a SEM. We observed the active movements of animals in vacuo (10−5—10−7 Pa) by coating them with a thin polymer membrane, NanoSuit®, made by polyoxyethylene (20) sorbitan monolaurate and found that the surface fine structure of the living organism is very different from that of traditionally fixed samples. After observation of the larvae of mosquitos, despite the high vacuum, we could rear them in normal culture conditions. We also found that the NanoSuit® and self-standing biocompatible films were able to produce by plasma polymerization from various monomer solutions. Our method will be useful for numerous applications, particularly in the electron microscopic observation of life sciences.