- 著者
- Gang Ma Lancui Zhang Kan Murakami Masaki Yahata Masaya Kato
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.QH-082, (Released:2023-08-11)
‘Miyagawa-wase’ (Citrus unshiu Marcow.), an early-season citrus variety, is rich in multiple nutrients and widely consumed in Japan. In ‘Miyagawa-wase’, when the pulp reaches maturity and is ready to eat, the peel is still a greenish color because of the relatively high temperature in the harvest season. In this study, to improve the coloration of ‘Miyagawa-wase’ peel, we treated the fruit with 1-naphthaleneacetic acid (NAA) after harvest. The results showed that postharvest treatment with NAA was effective to induce chlorophyll degradation and carotenoid accumulation in ‘Miyagawa-wase’ peel during storage. In the NAA treatment, the reduction in the chlorophyll contents after harvest was in parallel with decreases in the expression of chlorophyll biosynthetic genes (CitGGDR, CitCHLH, CitCHL27, CitPORA, and CitCAO) and an increase in chlorophyll degradation gene CitPPH. The contents of the major carotenoids, β-cryptoxanthin and 9-cis-violaxanthin, were increased by the NAA treatment through upregulation of the expression of carotenoid biosynthetic genes (CitPSY, CitPDS, CitZDS, CitLCYb2, and CitHYb) after harvest. In addition, it was found that the positive effect on degreening in the NAA treatment was inhibited by the ethylene antagonist 1-MCP. In the combination treatment using NAA and 1-MCP, the total chlorophyll content was much higher, while the contents of β-cryptoxanthin and 9-cis-violaxanthin were lower compared with NAA treatment alone, indicating that the acceleration of degreening by NAA may be caused by ethylene. The results presented in this study suggest that postharvest NAA treatment is an effective method for improving the peel coloration in early-season citrus varieties.
- 著者
- Anung Wahyudi Dinni Ariyani Gang Ma Ryosuke Inaba Chikako Fukasawa Ryohei Nakano Reiko Motohashi
- 出版者
- Japanese Society for Plant Cell and Molecular Biology
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- vol.35, no.4, pp.303-312, 2018-12-25 (Released:2018-12-31)
- 参考文献数
- 29
- 被引用文献数
- 10
In this study, two temperature-induced lipocalin genes SlTIL1 and SlTIL2, and a chloroplastic lipocalin gene SlCHL were isolated from ‘Micro-Tom’ tomato. The coding sequences of SlTIL1, SlTIL2 and SlCHL were 558, 558, and 1002 bp, respectively. By TargetP analysis, no characteristic transit peptides were predicted in the proteins of SlTIL1 and SlTIL2, while a chloroplastic transit peptide was predicted in the protein of SlCHL. The subcellular localization results indicated that SlTIL1 and SlTIL2 proteins were major localized in the plasma membrane, while SlCHL was localized in chloroplast. To understand the function of lipocalins, transgenic tomato over-expressed SlTIL1, SlTIL2 and SlCHL and their virus-induced gene silencing (VIGS) plants were generated. The phenotypes were significantly affected when the SlTIL1, SlTIL2 and SlCHL were over-expressed or silenced by VIGS, which suggested that the three lipocalins played important roles in regulating the growth and development of tomato. In addition, the level of ROS (O2− and H2O2) was low in SlTIL1, SlTIL2 and SlCHL over-expressed plants, while it was high in their silenced plants. The changes in the expression of SODs were consistent with the accumulations of ROS, which indicated that lipocalins might have an important role in abiotic oxidative stress tolerance in tomato plants. Especially SlTIL1 and SlTIL2 are localized around their membranes and protect them from ROS. The results will contribute to elucidating the functions of lipocalin in plants, and provide new strategies to improve the tolerance to abiotic stress in tomato plants.
- 著者
- Elizabeth L. HOMMEL Gang MA Heather C. ALLE
- 出版者
- (社)日本分析化学会
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.17, no.11, pp.1325-1329, 2001 (Released:2005-04-20)
- 参考文献数
- 32
- 被引用文献数
- 22 45
An important advance in surface science has been the evolution of sum frequency generation to the application of studying surface structure and chemistry of liquid surfaces at the molecular-level by probing the vibrational signatures of surface molecules. Recently, broad-bandwidth sum frequency generation (BBSFG) spectroscopy has become an important tool for investigating gas-solid interfaces. BBSFG spectroscopy allows, theoretically, a surface sum frequency spectrum to be acquired within one pulse of the laser. In this paper, the viability of BBSFG to study inherently small nonlinear response interfaces and the time-resolving capability of this surface-selective technology are demonstrated. Presented here are the first published accounts of spectra from a liquid surface utilizing the broad-bandwidth sum frequency technology with acquisition times as low as 500 milliseconds.