著者

湯川 夏夫

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.14, no.2, pp.54-60_2, 1972-04-25 (Released:2010-09-30)

参考文献数

10

著者

吉澤 拓也

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.63, no.2, pp.75-76, 2021-05-31 (Released:2021-06-05)

参考文献数

10

著者

有森 貴夫

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.63, no.2, pp.113-120, 2021-05-31 (Released:2021-06-05)

参考文献数

26

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In the protein crystallography, antibodies are frequently used as ‘crystallization chaperones’, where their binding facilitates production of high-quality diffracting crystals of complex macromolecules that are otherwise resistant to crystallization. To develop an ideal antibody fragment, we have designed a novel antibody fragment format, called ‘Fv-clasp’, that is a fusion of an anti-parallel coiled-coil structure derived from the hMst1 SARAH domain to the Fv fragment of an antibody. We have demonstrated that Fv-clasp has superior properties over conventional antibody fragments including Fab and single-chain Fv(scFv)in terms of producibility, stability, and crystallizability.

著者

岩田 忠久

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.55, no.3, pp.188-196, 2013-06-30 (Released:2013-07-02)

参考文献数

33

被引用文献数

2 1

---

Poly([R]-3-hydroxybutyrate)(P(3HB))and its copolymers are accumulated by a wide variety of microorganisms as intracellular carbon and energy materials, and are extensively studied as biodegradable and biocompatible thermoplastics. Recently, we succeeded in obtaining strong fibers and films by new drawing techniques from microbial polyesters produced by both wild-type and recombinant bacteria. The improvement of mechanical properties of fibers and films is due not only to the orientation of molecular chains but also to the generation of a planar zigzag conformation. The structure of strong fiber with tensile strength of over 1.0 GPa was analyzed by micro-beam X-ray diffraction and X-ray micro-tomography with synchrotron radiation. The strong fibers and films were completely degraded in environment or by extracellular PHB depolymerases. In this article, I present the processing, mechanical properties, highly ordered structure and biodegradability of strong fibers and films produced from microbial polyesters.

著者

高橋 健治 佐々木 聡 柳澤 佳寿美 木村 滋 表 和彦 平山 令明 中村 吉男

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.35, no.4, pp.302-305, 1993-08-25 (Released:2010-09-30)

著者

海老原 章郎 新海 暁男 中川 紀子 増井 良治 三木 邦夫 横山 茂之 倉光 成紀

出版者

The Crystallographic Society of Japan

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.48, no.6, pp.403-410, 2006-12-25 (Released:2010-09-30)

参考文献数

29

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The final goal of this research project is the understanding of all fundamental biological phenomena in terms of physical chemistry. As a model organism for the structural and functional studies, an extremely thermophilic bacterium, Thermus thermophilus HB8, is very promising because of the small genome size, the availability of genetic tools for functional analysis, and the thermostability of its proteins (http: //www.thermus.org/) . In this report, we summarize the recent progress of this research project toward the systems biology.

著者

黒岩 芳弘

出版者

The Crystallographic Society of Japan

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.47, no.2, pp.147-153, 2005-04-27 (Released:2010-09-30)

参考文献数

16

被引用文献数

1

---

Electron charge density distributions of perovskite-type dielectric oxides, PbTiO3 (ferroelectric), and PbZrO3 and PbHfO3 (antiferroelectric), have been investigated associated with the phase transitions, by analyzing high-energy synchrotron-radiation powder diffraction data by the maximum entropy method (MEM) /Rietveld method. Two distinctive structural features, that is, disordered Pb atom and anisotropic charge density distributions around O atom, are observed only in PbZrO3 and PbHfO3 in the cubic phase. These features can be a clue to understanding why PbZrO3 and PbHfO3 show antiferroelectric phase transition.

著者

丹治 裕美 大戸 梅治 清水 敏之

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.55, no.5, pp.285-289, 2013-10-31 (Released:2013-11-01)

参考文献数

16

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The Toll-like receptors(TLRs)are a family of pattern-recognition receptors that recognize pathogen-associated molecular patterns and activate innate immune system. TLR8 is activated by single-stranded RNA or synthetic imidazoquinoline compounds. We determined the crystal structures of unliganded and liganded TLR8 to clarify signaling and activation mechanism of TLR8.TLR8 monomer was ring-shaped structure in which N- and C-termini interacted directly. Both in the unliganded and liganded forms, TLR8 formed m-shaped dimer in which two C termini converged in the middle. Ligands were located in the two equivalent positions in the dimerization interface related by the non-crystallographic two-fold axis. The C-termini of the two TLR8 protomers were separated by 53 Å in the unliganded form; whereas, C-termini of the two monomers were brought into close proximity(~30 Å)in the liganded form, which would enable the subsequent dimerization of the TIR domains and downstream signaling.

著者

深町 共栄

出版者

The Crystallographic Society of Japan

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.19, no.2, pp.51-67, 1977-03-31 (Released:2010-09-30)

参考文献数

47

被引用文献数

2 2

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The anomalous scattering factors of X-rays have been described by the classical and quantum-field theory. Then the results are compared among three different calculations of f' (ω) and f″ (ω) for isolated atoms : (1) by Hönl (quantum theory), (2) by Parratt and Hempstead (semi-empirical theory) and (3) by Cromer and Liberman (relativistic quantum theory) . The fine structures of f' (ω) and f″ (ω) near the absorption edge are also reviewed from the viewpoint of the band theory and the dispersion relation.

著者

平山 令明 松浦 良樹

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.32, no.2, pp.25-27, 1990-04-30 (Released:2010-09-30)

著者

米倉 功治 眞木 さおり

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.59, no.2-3, pp.88-95, 2017-06-30 (Released:2017-07-01)

参考文献数

41

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Electron 3D crystallography is a useful method for structure analysis from tiny and thin crystals of membrane proteins and protein complexes, which often yield crystals too small or too thin for even the synchrotron X-ray beam and X-ray free electron laser. More importantly, it can visualize the charged states of amino-acid residues and metals, as the diffraction pattern formed by elastically scattered electrons is directly related to the distribution of Coulomb potential. Here we introduce the development of this technique and structure determination with charges, and discuss further applications including a suitable treatment of electron scattering factors of charged atoms.

著者

谷口 卓也

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.63, no.1, pp.31-37, 2021-02-28 (Released:2021-03-04)

参考文献数

16

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Molecular crystals seem rigid, fragile, and lack flexibility. However, in spite of such an appearance, this decade has witnessed flexible responses of molecular crystals, induced by external stimuli such as light, heat, humidity, mechanical force, and so on. Such molecular crystals belong to the family of soft crystals due to the flexible features and the response to gentle stimulus. This article reviews the history and recent advances of photo- and thermo-mechanical soft crystals.

著者

杉本 宏

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.59, no.4, pp.166-173, 2017-08-31 (Released:2017-08-31)

参考文献数

39

被引用文献数

1

---

Iron is an essential element for almost all living organism as it serves as a catalytic center for redox and metabolic reactions in many enzymes. Bacterial pathogens have evolved the efficient systems for the heme acquisition to survive in the host tissues and body fluids, because the heme is a rich source of iron. Pathogens grab the heme from the host hemoproteins and import it into cytoplasm by using many proteins including hemophore and ATP hydrolysis-driven transporter. In this review, recent discoveries in the molecular mechanism of the heme acquisition, membrane transport, degradation and sensing of the heme, with particular emphasis on the recognition of the heme and the dynamics of protein conformation from structural viewpoint.

著者

鈴木 光明 山田 道夫 前田 優 永瀬 茂 赤阪 健

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.58, no.2, pp.73-78, 2016-04-30 (Released:2016-04-30)

参考文献数

37

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For the structure determination of endohedral metallofullerenes (EMFs), X-ray crystallographic study is very powerful because it provides detailed information of the positions of encapsulated species as well as the cage structures. We have reported a number of X-ray crystal structures of EMFs and their derivatives, which are in good agreement with those suggested by theoretical calculations as well as NMR analyses. Herein we show the importance of interplay between experiments and theoretical calculations for the structure determination of EMFs by taking up La@C82, Gd@C82, and Sc2@C66 as the representative examples.

著者

緒方 英明

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.57, no.6, pp.344-349, 2015-12-31 (Released:2015-12-27)

参考文献数

21

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Hydrogen is known as an ultimate clean energy source and is thus discussed as a future sustainable energy carrier. Hydrogenases catalyze the reversible oxidation of the molecular hydrogen. We report the crystal structure analysis of [NiFe] hydrogenase from sulfate reducer at subatomic resolution. The structure reveals that the hydride bridge between nickel and iron at the active site and the possible proton bound site at the cysteine residue, resulting from the initial heterolytic splitting of dihydrogen by the enzyme. This finally clarifies the initial step in the mechanism of hydrogen conversion.

著者

水牧 仁一朗

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.59, no.6, pp.279-280, 2017-12-31 (Released:2017-12-31)

参考文献数

8

著者

田所 誠

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.59, no.6, pp.275-276, 2017-12-31 (Released:2017-12-31)

参考文献数

6

著者

瀬戸 誠

出版者

The Crystallographic Society of Japan

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.43, no.6, pp.405-412, 2001-12-31 (Released:2010-09-30)

参考文献数

31

被引用文献数

2 1

---

Recent progress of the study on condensed matter using nuclear resonant scattering of synchrotron radiation is reviewed. One of the most outstanding features of this method is the possibility to obtain the electronic and vibrational characters of the local environment of the resonant nucleus. The theoretical and experimental aspects and some of the experimental results are discussed.

著者

熊本 明仁 柴田 直哉 藤平 哲也 幾原 雄一

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.59, no.5, pp.246-251, 2017-10-31 (Released:2017-11-03)

参考文献数

20

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The atomic structures of liquid-phase bonded heterointerfaces between Al alloy and AlN single crystal was examined using aberration-corrected scanning transmission electron microscopy combined with high-sensitive energy-dispersive X-ray microanalysis. The formation of O-Mg-O monolayer structure was found at the interface, facilitating the bonding between the two dissimilar crystals during liquid-phase bonding processes. Understanding the spontaneous formation of such layered transition structures at the heterointerfaces will be a key for fabricating very stable liquid bonded Al alloy / AlN heterointerfaces.

著者

青柳 忍

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.59, no.5, pp.203-204, 2017-10-31 (Released:2017-11-03)

参考文献数

6