著者

Shinji Okada Ayano Katagiri Hiroto Saito Jun Lee Kinuyo Ohara Toshimitsu Iinuma Koichi Iwata

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.61, no.2, pp.370-378, 2019 (Released:2019-06-18)

参考文献数

40

被引用文献数

10

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Peripheral nerve injury can induce neuroplastic changes in the central nervous system and result in neuropathic pain. This study investigated functional involvement in dorsal paratrigeminal nucleus (dPa5) and nucleus tractus solitarii (NTS) neurons projecting to the parabrachial nucleus (PBN) after trigeminal nerve injury. Anatomical quantification was performed based on phosphorylated extracellular signal-regulated kinase (pERK) expression underlying orofacial neuropathic pain associated with infraorbital nerve chronic constriction injury (ION-CCI) in rats. ION-CCI rats exhibited heat and mechanical hypersensitivity in the ipsilateral upper lip. After injection of retrograde tracer fluorogold (FG) into the contralateral PBN, ION-CCI rats received capsaicin or noxious mechanical stimulation to the upper lip. The total number of FG-labeled neurons in dPa5 and NTS did not change after ION-CCI, and pERK expression in dPa5 did not differ between sham and ION-CCI rats. In the NTS contralateral to ION-CCI, the number of pERK-immunoreactive neurons and percentage of pERK-immunoreactive FG-labeled PBN projection neurons were increased after capsaicin stimulation in ION-CCI rats. The present findings suggest that enhanced noxious inputs from the NTS to the PBN after trigeminal nerve injury modulates PBN neuron activity, which accompanies the affective components of orofacial neuropathic pain.

著者

Kentaro Okamura Hiroyasu Koizumi Akihisa Kodaira Hiroshi Nogawa Takayuki Yoneyama

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.61, no.2, pp.358-363, 2019 (Released:2019-06-18)

参考文献数

21

被引用文献数

8

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The purpose of this study was to evaluate the surface properties and gloss of CAD/CAM composites after toothbrush abrasion testing. Four CAD/CAM composites for molars (Cerasmart 300, Estelite P block, Katana Avencia P block, and KZR-CAD HR3 Gammatheta), one CAD/CAM composite for premolars (Shofu Block HC Hard), and one feldspathic ceramics (Vitablocs Mark II) were assessed. Knoop hardness number, gloss, and surface roughness (Ra, Rz, and Sa) were measured before and after toothbrush abrasion testing. Knoop hardness number values were in the order Vitablocs Mark II > Katana Avencia P block > Estelite P Block > Shofu Block HC Hard > Cerasmart 300 > KZR-CAD HR3 Gammatheta. After testing, the gloss of Estelite P block and KZR-CAD HR3 Gammatheta was greatly decreased and surface roughness was greatly increased. Periodic recall and re-polishing may thus be necessary when these products are used clinically.

著者

Fumiaki Kimura Futoshi Komine Kei Kubochi Shogo Yagawa

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.61, no.2, pp.327-334, 2019 (Released:2019-06-18)

参考文献数

25

被引用文献数

2

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This study investigated bond strength of CAD/CAM-manufactured composite resin and ceramic veneers to a zirconia framework and analyzed the effect of treatments of veneer surfaces. A CAD/CAM resin-based (AVE) composite or lithium disilicate ceramic (IEC) block was used as the veneer material. AVE and IEC specimens were assigned to receive one of three surface treatments (n = 22): no surface treatment, acid-etching with 9.5% hydrofluoric acid gel, and airborne-particle abrasion with alumina particles. Zirconia disks and AVE or IEC specimens in each group were bonded with a resin-based luting agent, and shear bond strength of the specimens was measured at 0 and 20,000 thermocycles. Significant differences were assessed by the Steel-Dwass test for multiple comparisons and Mann-Whitney U-test (α = 0.05). As compared with other surface treatments, bond strengths were significantly higher at 0 and 20,000 thermocycles in the airborne-particle abraded AVE and acid-etched IEC specimens. Airborne-particle abrasion of the surface of AVE specimens increased bond strength between AVE veneers and zirconia frameworks, while hydrofluoric acid treatment enhanced bond strength between IEC veneers and zirconia frameworks.

著者

Toshiki Takamizawa Akimasa Tsujimoto Ryo Ishii Maho Ujiie Mami Kawazu Toshie Hidari Takayuki Suzuki Masashi Miyazaki

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.61, no.2, pp.276-283, 2019 (Released:2019-06-18)

参考文献数

33

被引用文献数

4

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The purpose of this study was to examine the effect of desensitizing dentifrices containing stannous fluoride (SnF2) on dentinal tubule occlusion. Two experimental dentifrices with the same ingredients but different SnF2 concentrations (Group II, 0.4% w/w; Group III, 0.454% w/w) were used; distilled water was used in the control group (Group I). Third molars were collected from Japanese and American dental patients. The crowns were removed and sectioned to obtain dentin discs, which were further cut into quarters. Thirty-six specimens each from Japanese and American patients were divided into three sets (n = 12 each) and assigned to each of the three treatment groups. The specimens were brushed for 10 s twice per day for 4 days. After treatment, the discs were observed by scanning electron microscopy, and the extent of dentinal tubule occlusion in the images was expressed on a five-point categorical scale. Group II and III specimens from Japanese and American patients showed greater dentinal tube occlusion than those from Group I, but the differences were not statistically significant. The present results suggest that both SnF2 concentrations mitigate dentin hypersensitivity, regardless of patient ethnicity.

著者

Hideyuki Imai Hiroyasu Koizumi Akihisa Kodaira Kentaro Okamura Shunsuke Akahane Hideo Matsumura

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.61, no.1, pp.178-183, 2019 (Released:2019-03-28)

参考文献数

16

被引用文献数

1

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This study evaluated the light intensity of light-emitting diode (LED) units and the effects of five laboratory polymerization units on hardness and flexural strength. Two indirect composite materials (Cesead N and Solidex Hardura) were polymerized with five units (α-Light II, Hyper LII, LED Cure Master, Twinkle LED, and α-Light V). The light intensity of the devices was measured with a spectroradiometer. After light exposure, Knoop hardness number, flexural strength, and elastic modulus were determined. Evaluation of light intensity, using a wavelength range of 400 to 500 nm, revealed that the α-Light V and Hyper LII units had the highest light intensity. For the top surface of the two composites, the Knoop hardness number was significantly higher for the α-Light V and Hyper LII. For the two composite materials, flexural strength did not differ among the five polymerizing units. The present results indicate that the Cesead N and Solidex Hardura composites can be adequately polymerized with laboratory LED units.

著者

Shinji Okada Hiroto Saito Yutaka Matsuura Lou Mikuzuki Shiori Sugawara Hiroki Onose Junichi Asaka Kinuyo Ohara Jun Lee Toshimitsu Iinuma Ayano Katagiri Koichi Iwata

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.61, no.1, pp.146-155, 2019 (Released:2019-03-28)

参考文献数

77

被引用文献数

2

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Bright light stimulation of the eye activates trigeminal subnucleus caudalis (Vc) neurons in rats. Sensory information is conveyed to the Vc via the trigeminal ganglion (TG). Thus, it is likely that TG neurons respond to photic stimulation and are involved in photic hypersensitivity. However, the mechanisms underlying this process are unclear. Therefore, the hypothesis in this study is bright light stimulation enhances the excitability of TG neurons involved in photic hypersensitivity. Expressions of calcitonin gene-related peptide (CGRP) and neuronal nitric oxide synthase (nNOS) were significantly higher in TG neurons from 5 min to 12 h after photic stimulation of the eye. Phosphorylation of extracellular signal-regulated kinase1/2 (pERK1/2) was enhanced in TG neurons within 5 min after photic stimulation, while pERK1/2 immunoreactivity in satellite glial cells (SGCs) persisted for more than 12 h after the stimulus. Activation of SGCs was observed from 5 min to 2 h. Expression of CGRP, nNOS, and pERK1/2 was observed in small and medium TG neurons, and activation of SGCs and pERK1/2-immunoreactive SGCs encircling large TG neurons was accelerated after stimulation. These results suggest that upregulation of CGRP, nNOS, and pERK1/2 within the TG is involved in photic hypersensitivity.

著者

Yasuyo Takahashi Kotoe Mayahara Rena Fushiki Rieko Matsuike Noriyoshi Shimizu

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

pp.18-0001, (Released:2019-02-01)

参考文献数

24

被引用文献数

4

---

High-magnitude mechanical strain inhibits bone nodule formation by reducing expression of bone morphogenetic protein-2 (BMP-2), Runt-related transcription factor 2 (Runx2), and muscle segment homeobox 2 (Msx2). Mechanical strain also induces production of proinflammatory factor prostaglandin E2 (PGE2) by osteoblasts. We measured the effect of mechanical strain-induced PGE2 production on bone nodule formation and expression levels of bone formation-related factors. Osteoblast-like cells isolated from fetal rat calvariae were loaded with 18% cyclic tension force (TF) for 48 h in the presence or absence of NS-398, a selective inhibitor of cyclooxygenase-2. To investigate the effect of TF-induced PGE2 on bone formation, bone nodule area on day 21 was measured by von Kossa staining. BMP-2, Runx2, and Msx2 expression levels were examined at 1 day after TF loading. Bone nodule formation was significantly inhibited by TF but was restored to control level by PGE2 inhibition. Furthermore, TF loading-induced reductions in expressions of these factors were restored to control level by PGE2 suppression. These results indicate that PGE2 production induced by high-magnitude mechanical strain inhibits bone nodule formation by reducing expression levels of bone formation-related factors.

著者

Janaina C. O. Sardi Cristiane Duque Flávia S. Mariano Iza T. A. Peixoto José F. Höfling Reginaldo B. Gonçalves

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.52, no.2, pp.177-185, 2010 (Released:2010-06-25)

参考文献数

64

被引用文献数

27 53

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Although the main reservoir of Candida spp. is believed to be the buccal mucosa, these microorganisms can coaggregate with bacteria in subgingival biofilm and adhere to epithelial cells. Such interactions are associated with the capacity of Candida spp. to invade gingival conjunctive tissue, and may be important in the microbial colonization that contributes to progression of oral alterations caused by diabetes mellitus, some medications, and immunosuppressive diseases such as AIDS. In addition, immune deficiency can result in proliferation of Candida spp. and germination of forms that are more virulent and have a higher capacity to adhere to and penetrate cells in host tissues. The virulence factors of Candida spp. increase host susceptibility to proliferation of these microorganisms and are likely to be important in the study of periodontal disease. Herein, we briefly review the literature pertaining to the role of Candida spp. in periodontal disease, and consider the main virulence factors, the host immune response to these microorganisms, and the effect of concomitant immunosuppressive conditions. (J Oral Sci 52, 177-185, 2010)

著者

Tomohiro Yasumitsu Osamu Shimizu Hiroshi Shiratsuchi Yusuke Miyake Yoshiyuki Yonehara

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.60, no.4, pp.595-600, 2018 (Released:2018-12-27)

参考文献数

33

被引用文献数

6

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The aim of this study was to determine the localization of aquaporin-5 (AQP5), transforming growth factor-β1 (TGF-β1) and laminin during regeneration of the rat submandibular gland. After duct ligation for 7 days, the regenerating glands were collected on days 0, 1, 3, 7, and 14 after ligation release to study the process of regeneration. Immunohistochemical staining revealed apical expression of AQP5 in many acinar cells, strong expression in intercalated ducts (ICDs) of the normal submandibular gland at Day 14, and strong expression in duct-like structures (DLSs) during regeneration from Day 0 to 7. However, a few AQP5-negative acinar cells were detected during regeneration. At Day 0, immunopositivity for TGF-β1 was detected in connective tissue. At Days 3 and 7 during regeneration, TGF-β1 immunostaining was observed in DLSs, which were surrounded by α-smooth muscle actin-positive thickened myoepithelial cells. Laminin staining was strong in the thickened basement membrane of DLSs at Day 3 during regeneration, but weak around acinar cells at Day 14. These findings suggest that TGF-β1 is involved in the environment around DLSs, myoepithelial cells and laminin, that DLSs have the same functional properties as ICDs, and that AQP5-negative acinar cells may be mucous cells.

著者

Takahisa Okubo Naoki Tsukimura Takashi Taniyama Manabu Ishijima Kourosh Nakhaei Naser M. Rezaei Makoto Hirota Wonhee Park Daisuke Akita Atsushi Tateno Tomohiko Ishigami Takahiro Ogawa

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.60, no.4, pp.567-573, 2018 (Released:2018-12-27)

参考文献数

23

被引用文献数

9

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Titanium mesh plate (Ti mesh) used for bone augmentation inadvertently comes into contact with medical gloves during trimming and bending. We tested the hypotheses that glove contact degrades the biological capability of Ti mesh and that ultraviolet treatment (UV) can restore this capability. Three groups of Ti mesh specimens were prepared: as-received (AR), after glove contact (GC), and after glove contact followed by UV treatment. The AR and GC meshes were hydrophobic, but GC mesh was more hydrophobic. AR and GC meshes had significant amounts of surface carbon, and Si content was higher for GC mesh than for AR mesh. UV mesh was hydrophilic, and carbon and silicon content values were significantly lower in this group than in the AR and GC groups. The number, alkaline phosphatase activity, and mineralization ability of attached osteoblasts were significantly lower in the GC group than in the AR group and markedly higher in the UV group than in the AR group. In conclusion, glove contact caused chemical contamination of Ti mesh, which significantly reduced its bioactivity. UV treatment restored bioactivity in contaminated Ti mesh, which outperformed even the baseline Ti mesh.

著者

Hisanori Ito Takashi Kifune Misa Ishiyama Satoko Iwasa Hiroki Takei Tomokazu Hasegawa Masatake Asano Tetsuo Shirakawa

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.60, no.4, pp.544-551, 2018 (Released:2018-12-27)

参考文献数

30

被引用文献数

5

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Hypoxia after traumatic injuries to a tooth is one of the causes of subsequent root resorption. Inflammatory cytokines produced under hypoxic conditions are associated with root resorption, but the mechanism has not been fully understood. In this study, the role of hypoxia-inducible factor-1 (HIF-1) signaling in the regulation of CCAAT (cytosine-cytosine-adenosine-adenosine-thymidine)/enhancer-binding protein-β (C/EBPβ) and the receptor activator of nuclear factor kappa-B ligand (RANKL) expressions in immortalized human periodontal ligament (PDL) cells was investigated. PDL cells cultured under a hypoxic condition showed an increase in the expression of C/EBPβ and RANKL messenger RNAs (mRNAs), whereas the expression of osteoprotegerin and HIF-1α mRNAs was unaffected. Hypoxia had no effects on the secretion of interleukin (IL)-1β, IL-6, IL-8, IL-17A, tumor necrosis factor-alpha, macrophage migration inhibitory factor, monocyte chemoattractant protein-1, and macrophage colony-stimulating factor in the culture media. Treatment of the cells with dimethyloxaloylglycine, a competitive HIF prolyl hydroxylase inhibitor, significantly increased the expression of C/EBPβ and RANKL mRNAs. This suggested that the hypoxia-induced elevation of C/EBPβ and RANKL mRNAs was dependent on the HIF-1 activity. PDL cells transfected with a specific small interfering RNA designed to target the C/EBPβ gene showed a significant suppression of the RANKL mRNA. These findings indicated that C/EBPβ may play an important role in tooth root resorption via RANKL activation in hypoxia-exposed PDL cells.

著者

Takashi Kifune Hisanori Ito Misa Ishiyama Satoko Iwasa Hiroki Takei Tomokazu Hasegawa Masatake Asano Tetsuo Shirakawa

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.60, no.4, pp.519-525, 2018 (Released:2018-12-27)

参考文献数

32

被引用文献数

11

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Hypoxia induces complex cellular responses that are mediated by a key transcription factor, hypoxia-inducible factor-1 (HIF-1). HIF-1 promotes production of cytokines and angiogenic factors and contributes to recovery of injured tissues. In the present study, expressions of angiogenin (ANG) and vascular endothelial growth factor (VEGF), which are potent angiogenic factors in mammalian tissues, were examined in immortalized fibroblasts exposed to hypoxia. After 24 h of exposure to hypoxia, ANG and VEGF mRNAs expressions were significantly elevated in periodontal ligament (PDL) fibroblasts but not in embryonic fibroblasts. Hypoxia also increased productions of ANG and VEGF proteins in PDL fibroblasts. HIF-1α mRNA expression was not affected by hypoxia in either fibroblast, although HIF-1α protein expression was enhanced after exposure to hypoxia. Treatment of PDL fibroblasts with dimethyloxaloylglycine, a prolyl hydroxylase inhibitor that stabilizes the HIF-1α protein, significantly increased expressions of ANG and VEGF mRNAs under normoxia. This suggests that stabilization of HIF-1α is crucial for upregulation of ANG and VEGF in PDL fibroblasts. These results indicate that, under hypoxic conditions, HIF-1α upregulates synthesis of ANG and VEGF in PDL fibroblasts and promotes angiogenesis.

著者

Tatsuro Suzuki Masahiro Kondo Ikuko Shibuta Hidekazu Nagashima Naoyuki Sugano Shuichi Sato Koichi Iwata

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.60, no.4, pp.500-506, 2018 (Released:2018-12-27)

参考文献数

25

被引用文献数

1

---

The inferior alveolar nerve (IAN) comprises several types of sensory fibers. To clarify whether each type of primary afferent is regenerated comparably after injury, we developed a model of complete IAN transection (IANX) in mice. A retrograde tracer, fluoro-gold, injected into the mental skin was transferred to the cell bodies of a subset of isolectin B4 (IB4)-binding (non-peptidergic C) or CGRP-positive (peptidergic C) neurons at 2 weeks post-axotomy, indicating that the injured C afferents had regenerated anatomically. IANX led to a decrease of IB4-binding and CGRP immunoreactivity (IR) in the trigeminal ganglion (TG) and within the trigeminal spinal subnucleus caudalis (Vc) (i.e. terminals of the central branch of TG neurons). Two weeks after IANX, the reduction in IB4-binding activity and CGRP expression in the TG recovered to the control level; however, IB4-binding within the Vc did not, suggesting that central branch non-peptidergic neurons remained impaired. Two weeks after IANX, pinching or heat stimulus-induced extracellular signal-regulated kinase phosphorylation (pERK) was restored to the control level, but in the case of pinch stimulation the distribution pattern of pERK-IR cells was altered in the Vc. Taken together, our results support the possibility that peptidergic neurons regenerate more efficiently than non-peptidergic neurons after trigeminal nerve injury.

著者

Hiroki Komiya Kohei Shimizu Kae Ishii Hiroshi Kudo Teinosuke Okamura Kohei Kanno Masamichi Shinoda Bunnai Ogiso Koichi Iwata

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.60, no.4, pp.493-499, 2018 (Released:2018-12-27)

参考文献数

23

被引用文献数

28

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Pulpitis often causes referred pain in opposing teeth. However, the precise mechanism underlying ectopic pain associated with tooth-pulp inflammation remains unclear. We performed the present study to test the hypothesis that functional interactions between satellite glial cells (SGCs) and trigeminal ganglion (TG) neurons are involved in ectopic orofacial pain associated with tooth-pulp inflammation. Digastric muscle electromyograph (D-EMG) activity elicited by administration of capsaicin into the upper second molar pulp (U2) was analyzed to evaluate noxious reflex responses. D-EMG activity was significantly increased in rats with lower first molar (L1) inflammation relative to saline-treated rats. Significantly increased expression of glial fibrillary acid protein (GFAP), a marker of activated glial cells, and connexin 43 (Cx43), a gap-junction protein, was observed in activated SGCs surrounding U2-innervating TG-neurons after L1-pulp inflammation. Daily administration of Gap26, a Cx43-inhibiting mimetic peptide, into the TG significantly suppressed capsaicin-induced D-EMG activity enhancement and reduced the percentage of fluorogold-labeled (U2-innervated) cells that were surrounded by GFAP-immunoreactive (IR) and Cx43-IR cells after L1-pulp inflammation. These findings indicate that tooth-pulp inflammation induces SGC activation and subsequent spread of SGC activation in the TG via Cx43-containing gap junctions. Thus, remote neuron excitability becomes enhanced in the TG following tooth-pulp inflammation, resulting in ectopic tooth-pulp pain in the contralateral tooth.

著者

Akiko Okada-Ogawa Naohiko Sekine Kosuke Watanabe Ryutaro Kohashi Sayaka Asano Koich Iwata Yoshiki Imamura

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

pp.17-0453, (Released:2018-12-20)

参考文献数

53

被引用文献数

8

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Assessment and treatment of masticatory myofascial pain syndrome (MPS) are not standardized and remain controversial. We examined whether muscle hardness was useful for evaluating masticatory MPS and analyzed the effectiveness of treatments such as stretching and massage (SM) and trigger point injection (TPI). Twenty healthy volunteers and 20 MPS patients were enrolled. MPS patients were divided into TPI and SM treatment groups. Hardness of masticatory muscle with a taut band (TB) and change in hardness were evaluated after SM and TPI treatments. Hardness values were significantly higher in muscle including a TB (TB point) than in the muscle of healthy controls. Visual analogue scale scores were significantly lower after SM and TPI treatments, and hardness of the TB point was significantly lower after SM but not after TPI. These results suggest that measurement of muscle hardness, including the TB, is useful for evaluating masticatory MPS. However, TPI analgesia might not be caused by change in muscle hardness. The mechanisms underlying the effects of SM and TPI on reducing pain in MPS may differ and thus warrant further research.

著者

Verónica Zavala-Alonso Juan P. Loyola-Rodríguez Humberto Terrones Nuria Patiño-Marín Gabriel A. Martínez-Castañón Kenneth Anusavice

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.54, no.1, pp.93-98, 2012 (Released:2012-03-30)

参考文献数

28

被引用文献数

9 18

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The aim of this study was to analyze the molecular structure of enamel with fluorosis using micro-Raman spectroscopy and compare it with that of healthy enamel. Eighty extracted human molars were classified into four fluorosis groups according to the Thylstrup-Fejerskov Index (TFI) [TFI: 0, Healthy enamel; 1-3, mild; 4-5, moderate; 6-9, severe fluorosis]. All samples were analyzed by micro-Raman spectroscopy. The integral areas of ν1 (960 cm−1) phosphate peak as well as B-type carbonate peak (1070 cm−1) were obtained to analyze structural differences among the specimens. Although the differences were not statistically significant (P > 0.05), the mean of integral areas of ν1 phosphate peak among groups indicated greater mineralization in the severe fluorosis group. However, there were statistically significant differences in the intensities, and the integral areas of B-type carbonate peak among groups (P < 0.05). Therefore, mineralization of the carbonate peak at 1070 cm−1 decreased significantly in fluorotic groups, suggesting that carbonate ions are easily dissolved in the presence of fluoride. Although structurally fluorotic teeth are not more susceptible to dental caries, serious alteration in its surface topography may cause retention of bacterial plaque and formation of enamel caries. Micro-Raman spectroscopy is a useful tool for analyzing the molecular structure of healthy and fluorotic human enamel. (J Oral Sci 54, 93-98, 2012)

著者

Kae Ishii Keisuke Hatori Osamu Takeichi Kosuke Makino Kazuma Himi Hiroki Komiya Bunnai Ogiso

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

pp.17-0439, (Released:2018-11-15)

参考文献数

31

被引用文献数

1

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It has been reported that Forkhead box transcription factor class O3a (Foxo3a) is expressed in rheumatoid arthritis, a chronic inflammatory condition accompanied by bone resorption, and plays a role in its pathology. However, it has remained unclear whether Foxo3a is involved in the pathogenesis of periapical granulomas. The present study was performed to compare the expression of Foxo3a in periapical granulomas and healthy gingival tissues. Samples were obtained surgically from patients, and subjected to hematoxylin-eosin staining for histopathologic diagnosis. Two-color immunofluorescence staining was also performed using antibodies against Foxo3a and markers for three types of inflammatory cells: neutrophils, T lymphocytes, and B lymphocytes. This revealed that Foxo3a was expressed in all three cell types in periapical granulomas but not in healthy gingival tissues. Foxo3a was expressed in 82.1%, 78.3%, and 77.5% of neutrophils, T lymphocytes, and B lymphocytes, respectively, and statistical analysis using the Kruskal-Wallis test followed by the Steel-Dwass test showed no significant difference of Foxo3a expression among the three cell types. Our results suggest that Foxo3a transcription factors may be involved in the pathogenesis of periapical granulomas.

著者

Yusuke Miyake Keiji Shinozuka Kosuke Ueki Jun Teraoka Manabu Zama Shouhei Ogisawa Yasuhisa Shinozaki Junya Aoki Keiichi Yanagawa Osamu Shimizu Tadayoshi Kaneko Morio Tonogi Hidero Ohki

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

pp.17-0317, (Released:2018-11-15)

参考文献数

16

被引用文献数

4

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To better understand the clinical features of mass lesions of the tongue, we retrospectively evaluated frequency, recurrence rate, and complications in 296 patients who had undergone surgery for such lesions. The diagnoses were fibroma (43.6%), mucous cyst (14.2%), papilloma (11.8%), hemangioma (7.8%), granuloma (6.4%), lipoma (1.4%), schwannoma (1.0%), ectopic tonsil (0.7%), and other (13.2%). Recurrence was noted in two patients (0.7%). Twenty-two patients (7.4%) developed surgical complications, including lingual nerve paralysis (6.4%), glossodynia (0.6%), and postoperative infection (0.3%). Lingual nerve paralysis was observed in the ventral portion (42.1%) of the tongue, apex (36.8%), lateral border (10.5%), and dorsum (10.5%). When all sites were considered together, there was no significant difference in the number of patients presenting with lingual nerve paralysis (P = 0.075). However, there were significant differences in lingual nerve paralysis at the lateral border (P < 0.05), apex (P < 0.05), and dorsum (P < 0.001) but not at the ventral portion (P > 0.05) in the size of the patients with versus without it which suggests that the risk of lingual nerve paralysis is higher at the ventral tongue, regardless of tumor size. These results shed light on the clinical features of mass lesions of the tongue.

著者

Michiko Ichinohe Mitsuru Motoyoshi Mizuki Inaba Yasuki Uchida Mari Kaneko Reiko Matsuike Noriyoshi Shimizu

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

pp.17-0377, (Released:2018-10-26)

参考文献数

35

被引用文献数

19

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This study investigated the stability of mini-screws placed in the median palate. The study included 25 patients (7 males, 18 females; mean age, 23.4 ± 5.6 years; age range, 15.0-34.5 years) who had mini-screws placed during orthodontic treatment at Nihon University School of Dentistry Dental Hospital. Mini-screws (diameter, 2.0 mm; length, 9.0 mm) were placed in the median palatal region; the first screw was inserted mesiodistally at the distal contact of the maxillary first molar, and the second screw was placed 6-9 mm mesial to the first screw. Immediately after placement, the placement sites were carefully examined with cone-beam computed tomography and a Periotest device. Screw stability was not related to perforation of the nasal cavity, patient age, or patient sex. The success rate was significantly higher in patients with screw-suture distances of 1.5-2.7 mm than in those with distances of 0-1.4 mm. Moreover, mini-screws could be stabilized when palatal cortical bone thickness was ≥1.5 mm. The success rate was significantly higher in the group with insertion depths of ≥4.5 mm. These results indicate that primary stability of mini-screws requires sufficient cortical bone thickness, insertion depth, and screw-suture distance.

著者

Elaheh Vahid-Dastjerdi Ali Borzabadi-Farahani Mina Mahdian Nazila Amini

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.52, no.3, pp.455-461, 2010 (Released:2010-09-24)

参考文献数

56

被引用文献数

21 48

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To investigate the prevalence, characteristics (ie, malocclusion, location, type), and sex distribution of hypodontia in an Iranian orthodontic population. A retrospective study was conducted using periapical and panoramic radiographs and study models of 1,751 subjects attending university orthodontic clinics (870 females, 881 males, age 9-27 years). The Chi-square test was used to analyze differences in the distribution of hypodontia, after stratification by sex and malocclusion type. A total of 197 congenitally missing teeth were observed in 160 patients (9.1%; 74 boys and 86 girls); there were no statistically significant differences between sexes (Chi-square = 0.832, P = 0.36). Hypodontia was more common in patients with Class III malocclusion (45.2%), and was more prevalent in the maxilla (71%) than in the mandible (29%). Maxillary lateral incisors (35.6%) and maxillary second premolars (13.0%) were the most commonly missing teeth, followed by mandibular lateral incisors (9.6%) and mandibular second premolars (8.2%). The prevalence of missing teeth was higher in the anterior segment (incisors and canines) than in the posterior segment (premolars and molars). The prevalence of oligodontia was 0.34%. The prevalence and characteristics of the most frequently missing teeth accorded with the findings of most studies conducted in other countries. (J Oral Sci 52, 455-461, 2010)