著者

Hirofumi Tsuru Hidekazu Ishida Jun Narita Ryo Ishii Hidehiro Suginobe Yoichiro Ishii Renjie Wang Shigetoyo Kogaki Masaki Taira Takayoshi Ueno Yohei Miyashita Hidetaka Kioka Yoshihiro Asano Yoshiki Sawa Keiichi Ozono

出版者

The Japanese Circulation Society

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

vol.85, no.5, pp.677-686, 2021-04-23 (Released:2021-04-23)

参考文献数

38

被引用文献数

8

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Background:Restrictive cardiomyopathy (RCM) is characterized by impaired ventricular relaxation. Although several mutations were reported in some patients, no mutations were identified in cardiomyocyte expressing genes of other patients, indicating that pathological mechanisms underlying RCM could not be determined by cardiomyocytes only. Cardiac fibroblasts (CFs) are a major cell population in the heart; however, the pathological roles of CFs in cardiomyopathy are not fully understood.Methods and Results:This study established 4 primary culture lines of CFs from RCM patients and analyzed their cellular physiology, the effects on the contraction and relaxation ability of healthy cardiomyocytes under co-culture with CFs, and RNA sequencing. Three of four patients hadTNNI3mutations. There were no significant alterations in cell proliferation, apoptosis, migration, activation, and attachment. However, when CFs from RCM patients were co-cultured with healthy cardiomyocytes, the relaxation velocity of cardiomyocytes was significantly impaired both under direct and indirect co-culture conditions. RNA sequencing revealed that gene expression profiles of CFs in RCM were clearly distinct from healthy CFs. The differential expression gene analysis identified that several extracellular matrix components and cytokine expressions were dysregulated in CFs from RCM patients.Conclusions:The comprehensive gene expression patterns were altered in RCM-derived CFs, which deteriorated the relaxation ability of cardiomyocytes. The specific changes in extracellular matrix composition and cytokine secretion from CFs might affect pathological behavior of cardiomyocytes in RCM.

著者

Ryo Ishii Ryuichiro Higashinaka Koh Mitsuda Taichi Katayama Masahiro Mizukami Junji Tomita Hidetoshi Kawabata Emi Yamaguchi Noritake Adachi Yushi Aono

出版者

Information Processing Society of Japan

雑誌

Journal of Information Processing (ISSN:18826652)

巻号頁・発行日

vol.29, pp.30-44, 2021 (Released:2021-01-15)

参考文献数

30

被引用文献数

3

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Starting from their early years, many persons dream of being able to chat with their favorite anime characters. To make such a dream possible, we propose an efficient method for constructing a system that enables users to text chat with existing anime characters. We tackled two research problems to generate verbal and nonverbal behaviors for a text-chat agent system utilizing an existing character. A major issue in creating verbal behavior is generating utterance text that reflects the personality of existing characters in response to any user questions. To cope with this problem we propose use of role play-based question-answering to efficiently collect high-quality paired data of user questions and system answers reflecting the personality of an anime character. We also propose a new utterance generation method that uses a neural translation model with the collected data. Rich and natural expressions of nonverbal behavior greatly enhance the appeal of agent systems. However, not all existing anime characters move as naturally and as diversely as humans. Therefore, we propose a method that can automatically generate whole-body motion from spoken text in order to give the anime characters natural, human-like movements. In addition to these movements, we try to add a small amount of characteristic movement on a rule basis to reflect personality. We created a text-dialogue agent system of a popular existing anime character using our proposed generation methods. As a result of a subjective evaluation of the implemented system, our methods for generating verbal and nonverbal behavior improved the impression of the agent's responsiveness and reflected the personality of the character. Since generating characteristic motions with a small amount of characteristic movement on the basis of heuristic rules was not effective, our proposed motion generation method which can generate the average motion of many people, is useful for generating motion for existing anime characters. Therefore, our proposed methods for generating verbal and nonverbal behaviors and the system-construction method are likely to prove a powerful tool for achieving text-dialogue agent systems for existing characters.

著者

Koji HAYASHI Ryo ISHII Tosihiki TAKAMIZAWA Shunichi SUDA Ryota AOKI Kana HAYASHI Atsushi KAMIMOTO Masashi MIYAZAKI

出版者

The Japanese Society for Dental Materials and Devices

雑誌

Dental Materials Journal (ISSN:02874547)

巻号頁・発行日

pp.2023-073, (Released:2023-11-23)

参考文献数

29

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This study aimed to evaluate the influence of surface pretreatment on the shear bond strength of resin luting cement to saliva-contaminated resin core foundation. The surface free energy (γS) of the adherent surfaces was examined. The two-way analysis of variance revealed that the surface pretreatment and storage conditions had a significant effect on the strength of the bond to resin core foundation. The γS values of the saliva-contaminated group were significantly lower than those of the other groups, and they tended to improve after surface pretreatment. The tendency of improvement in γS values differed depending on the type of pretreatment agents. Surface treatment with solutions containing functional monomers is effective in removing saliva contaminants from the resin core foundation surfaces and in creating an effective bonding surface for the resin luting cement.

著者

Toshiki TAKAMIZAWA Eizo HIROKANE Keiichi SAI Ryo ISHII Ryota AOKI Wayne W. BARKMEIER Mark A. LATTA Masashi MIYAZAKI

出版者

The Japanese Society for Dental Materials and Devices

雑誌

Dental Materials Journal (ISSN:02874547)

巻号頁・発行日

pp.2022-130, (Released:2022-11-08)

参考文献数

42

被引用文献数

2

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This study investigated the enamel and dentin bond durability of a two-step adhesive system, using a universal adhesive-derived primer (G2-Bond Universal [GU]), and compared it with the two conventional, two-step, self-etch adhesive systems (Clearfil SE Bond 2 [CS] and OptiBond XTR [OX]) under thermal cycling (TC) and long-term water storage (WS). The shear bond strengths to bovine enamel and dentin were determined using the etch-and-rinse and self-etch modes. Twelve specimens per test group were prepared and further divided into the following subgroups: 1) subjected to TC, 2) long-term WS, and 3) WS for 24 h (baseline). The enamel and dentin bond durability of GU was superior to those of CS and OX in different etching modes and under different degradation conditions. The latest two-step adhesive system, which utilizes universal adhesives’ benefits in its primer, might offer durable clinical bonding performances and can be widely used in a clinical setting.

著者

Hajime OOUCHI Toshiki TAKAMIZAWA Tomohiko TAMURA Ryo ISHII Akimasa TSUJIMOTO Masashi MIYAZAKI Nobuya KITAHARA

出版者

The Japanese Society for Dental Materials and Devices

雑誌

Dental Materials Journal (ISSN:02874547)

巻号頁・発行日

pp.2020-381, (Released:2021-04-20)

参考文献数

28

被引用文献数

5

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This study investigates the flexural properties, Knoop hardness number (KHN), and wear behavior of computer-aided design and computer-aided manufacturing (CAD/CAM) resin blocks and compares these values with those of conventional resin composites for direct restoration (RCDs). Four CAD/CAM resin blocks and two RCDs are compared. Ten specimens per material are used to obtain the flexural properties according to ISO 6872 specifications, and five specimens per material are used for KHN measurement. For sliding impact wear testing, twelve specimens are prepared. Confocal laser scanning microscopy (LSM) is used to determine maximum facet depth and volume loss. Polished material surfaces and worn surfaces after wear testing are observed using scanning electron microscopy and LSM. While measured flexural properties and KHN are found to be material-dependent, CAD/CAM resin blocks exhibit higher wear resistance than RCDs. CAD/CAM resin blocks polymerized at high-pressure and high-temperature are found to exhibit wear behaviors far superior to those of RCDs.

著者

Toshie HIDARI Toshiki TAKAMIZAWA Arisa IMAI Eizo HIROKANE Ryo ISHII Akimasa TSUJIMOTO Takayuki SUZUKI Masashi MIYAZAKI

出版者

The Japanese Society for Dental Materials and Devices

雑誌

Dental Materials Journal (ISSN:02874547)

巻号頁・発行日

pp.2019-154, (Released:2020-02-29)

参考文献数

37

被引用文献数

14

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This study aimed to determine the role of the 10-methacryloyloxydecyl dihydrogen phosphate (MDP) in dentin bond durability of universal adhesive in etch-&-rinse mode through bond strength tests after different degradation conditions. This study used the MDP-containing universal adhesive, Clearfil Universal Bond Quick (CU), and an experimental adhesive (NM) made with the same ingredients as CU, excluding MDP. Shear bond strength (SBS) to dentin were obtained in etch-&-rinse mode with or without MDP. CU in self-etch mode was used as a comparison. The groups were divided into three types: 1) subjected to thermal cycling; 2) subjected to long-term water storage; and 3) stored in water for 24 h, as a baseline. The discrepancies in the mean SBS of the CU and NM groups become prominent with prolonged degradation periods. This study indicates that the MDP in universal adhesive might play a key role in enhancing dentin bond quality even when etch-&-rinse mode is used.

著者

Shunsuke AKIBA Toshiki TAKAMIZAWA Akimasa TSUJIMOTO Nobuyuki MORITAKE Ryo ISHII Wayne W BARKMEIER Mark A LATTA Masashi MIYAZAKI

出版者

The Japanese Society for Dental Materials and Devices

雑誌

Dental Materials Journal (ISSN:02874547)

巻号頁・発行日

pp.2018-300, (Released:2019-06-21)

参考文献数

40

被引用文献数

11

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We investigated the influence of different curing modes on the mechanical properties and wear behavior of dual-cure provisional resin-based composites (DCPRs). Three DCPRs and a self-curing bis-acryl provisional resin-based composite were used. Flexural strength (σF), elastic modulus (E), resilience (R), and fracture toughness (KIC) were measured. The specimens were fabricated with and without light irradiation, stored in distilled water at 37°C for 24 h, and subjected to 5,000 or 10,000 thermal cycles. For sliding impact wear testing, 12 specimens were prepared with and without light irradiation. The maximum facet depth and volume loss were determined using a noncontact profilometer. Some of the mechanical properties and wear behavior of DCPRs are affected by light irradiation. This study indicated that proper light irradiation is important in polymerization process of the DCPRs to enhance the wear resistance and some mechanical properties.

著者

Toshiki Takamizawa Akimasa Tsujimoto Ryo Ishii Maho Ujiie Mami Kawazu Toshie Hidari Takayuki Suzuki Masashi Miyazaki

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.61, no.2, pp.276-283, 2019 (Released:2019-06-18)

参考文献数

33

被引用文献数

4

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The purpose of this study was to examine the effect of desensitizing dentifrices containing stannous fluoride (SnF2) on dentinal tubule occlusion. Two experimental dentifrices with the same ingredients but different SnF2 concentrations (Group II, 0.4% w/w; Group III, 0.454% w/w) were used; distilled water was used in the control group (Group I). Third molars were collected from Japanese and American dental patients. The crowns were removed and sectioned to obtain dentin discs, which were further cut into quarters. Thirty-six specimens each from Japanese and American patients were divided into three sets (n = 12 each) and assigned to each of the three treatment groups. The specimens were brushed for 10 s twice per day for 4 days. After treatment, the discs were observed by scanning electron microscopy, and the extent of dentinal tubule occlusion in the images was expressed on a five-point categorical scale. Group II and III specimens from Japanese and American patients showed greater dentinal tube occlusion than those from Group I, but the differences were not statistically significant. The present results suggest that both SnF2 concentrations mitigate dentin hypersensitivity, regardless of patient ethnicity.

著者

Ryosuke Murayama Yuko Nagura Kabun Yamauchi Nobuyuki Moritake Masayoshi Iino Ryo Ishii Hiroyasu Kurokawa Masashi Miyazaki Yumiko Hosoya

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

pp.17-0256, (Released:2018-07-09)

参考文献数

40

被引用文献数

5

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We used optical coherence tomography to examine the effect of a coating material containing surface reaction-type pre-reacted glass-ionomer (S-PRG) filler on primary enamel demineralization in 18 extracted human primary teeth. The pulp was removed, and each tooth was ultrasonically cleaned with distilled water. Six teeth were treated with 0.1-M lactic acid buffer solution (De group). In the second group (n = 6), a thin film of coating material was applied before demineralization (PRG group). A third group (Control group; n = 6) was maintained in artificial saliva. Using optical coherence tomography, we measured peak signal intensity (dB) and width at 1/e2 (µm) at predetermined locations on the enamel surface and calculated integrated values. All data were analyzed with ANOVA and the Tukey-Kramer test (α = 0.05). Although changes in integrated values differed between groups, there was a small but significant increase in the Control group and a small but significant decrease in the De group. In the PRG group, integrated values were significantly higher at 7 days after the start of the experiment and significantly increased thereafter. Our findings indicate that a coating material containing S-PRG fillers may prevent primary enamel demineralization.

著者

Fumi YOSHIDA Akimasa TSUJIMOTO Ryo ISHII Kie NOJIRI Toshiki TAKAMIZAWA Masashi MIYAZAKI Mark A. LATTA

出版者

日本歯科理工学会

雑誌

Dental Materials Journal (ISSN:02874547)

巻号頁・発行日

vol.34, no.6, pp.855-862, 2015-11-27 (Released:2015-12-01)

参考文献数

28

被引用文献数

2 37

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This study investigates the influence of surface treatment of contaminated lithium disilicate and leucite glass ceramic restorations on the bonding efficacy of universal adhesives. Lithium disilicate and leucite glass ceramics were contaminated with saliva, and then cleaned using distilled water (SC), or 37% phosphoric acid (TE), or hydrofluoric acid (CE). Specimens without contamination served as controls. The surface free energy was determined by measuring the contact angles formed when the three test liquids were placed on the specimens. Bond strengths of the universal adhesives were also measured. Saliva contamination and surface treatment of ceramic surfaces significantly influenced the surface free energy. The bond strengths of universal adhesives were also affected by surface treatment and the choice of adhesive materials. Our data suggest that saliva contamination of lithum disilicate and leucite glass ceramics significantly impaired the bonding of the universal adhesives, and reduced the surface free energy of the ceramics.